Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Prion Protein Genotypes in Pakistani Goats

  • Babar, M.E. (University of Veterinary and Animal Sciences) ;
  • Nawaz, M. (University of Veterinary and Animal Sciences) ;
  • Nasim, A. (COMSTECH) ;
  • Abdullah, M. (University of Veterinary and Animal Sciences) ;
  • Imran, M. (University of Veterinary and Animal Sciences) ;
  • Jabeen, R. (University of Veterinary and Animal Sciences) ;
  • Chatha, S.A. (University of Veterinary and Animal Sciences) ;
  • Haq, A.U. (University of Veterinary and Animal Sciences) ;
  • Nawaz, A. (University of Veterinary and Animal Sciences) ;
  • Mustafa, H. (University of Veterinary and Animal Sciences) ;
  • Nadeem, A. (University of Veterinary and Animal Sciences)
  • Received : 2007.08.27
  • Accepted : 2008.02.27
  • Published : 2008.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

The PCR-amplified prion protein (PrP) gene was sequenced to determine the frequency of scrapie-associated as well as novel PrP genotypes in 72 healthy goats representing five breeds. A total of six genotypes were detected, resulting from the three reported 143 (H/R), 154 (R/H) and 240 (S/P) and the two novel 39 (S/R) and 185 (I/F) amino acid polymorphisms. Of the four silent mutations 42 (a$\rightarrow$g), 138 (c$\rightarrow$t), 231 (c$\rightarrow$a) and 237 (g$\rightarrow$c) detected in this study, 237 (g$\rightarrow$c) is novel. A genotype (SIP/RFP) harboring three amino acid polymorphisms 39 (S/R), 185 (I/F) and 240 (S/P) was found in few goats. Although both scrapie-associated genotypes with 143 (H/R) and 154 (R/H) polymorphisms and others with 39 (S/R), 185 (I/F) and 240 (S/P) polymorphisms were present in the studied Pakistani goats, their frequency was lower than that of the wild-type genotype SHRIS/SHRIS (34.7%). These results emphasize the need for further sequencing of the PrP gene in a large number of goats representing the five studied breeds, so that overall PrP variability can be assessed in these breeds in research addressing future concerns about scrapie.

Keywords

References

  1. Acutis, P. L., A. Bossers, J. Priem, M. V. S. Riina, Peletto, M. Mazza, C. Casalone, G. Forloni, G. Ru and M. Caramelli. 2006. Identification of prion protein gene polymorphisms in goats from Italian scrapie outbreaks. J. Gen. Virol. 87:1029-1033. https://doi.org/10.1099/vir.0.81440-0
  2. Agrimi, U., M. Conte, L. Morelli, M. A. Di Bari, G. Di Guardo, C. Ligios, G. Antonucci, G. M. Aufiero, N. Pozzato, F. Mutinelli, R. Nonno and G. Vaccari. 2003. Animal transmissible spongiform encephalopathies and genetics. Vet. Res. Commun. 27(Suppl. 1):31-38. https://doi.org/10.1023/B:VERC.0000014115.18327.26
  3. Baylis, M. and W. Goldmann. 2004. The genetics of scrapie in sheep and goats. Curr. Mol. Med. 4:385-396. https://doi.org/10.2174/1566524043360672
  4. Billinis, C., C. H. Panagiotidis, V. Psychas, S. Argyroudis, A. Nicolaou, S. Leontides, O. Papadopoulos and T. Sklaviadis. 2002. Prion protein gene polymorphisms in natural goat scrapie. J. Gen. Virol. 83:713-721. https://doi.org/10.1099/0022-1317-83-3-713
  5. Collinge, J. A. R. 2007. A general model of prion strains and their pathogenicity. Sci. 318:930-936. https://doi.org/10.1126/science.1138718
  6. Goldmannn, W., A. Chong, J. Foster, J. Hope and N. Hunter. 1998. The shortest known prion protein gene allele occurs in goats, has only three octapeptide repeats and is non-pathogenic. J. Gen. Virol. 79:3173-3176. https://doi.org/10.1099/0022-1317-79-12-3173
  7. Goldmannn, W., T. Martin, J. Foster, S. Hughes, G. Smith, K. Hughes, M. Dawson and N. Hunter. 1996. Novel polymorphisms in the caprine PrP gene: a codon 142 mutation associated with scrapie incubation period. J. Gen. Virol. 77:2885-2891. https://doi.org/10.1099/0022-1317-77-11-2885
  8. Goldmannn, W., M. Perucchini, A. Smith and N. Hunter. 2004. Genetic variability of the PrP gene in a goat herd in the UK. Vet. Rec. 155:177-178. https://doi.org/10.1136/vr.155.6.177
  9. Govt of Pakistan, Economic survey of Pakistan, 2006.
  10. Grimberg, J., S. Nawoschik, L. Belluscio, R. Mckee, A. Turk and A. Eiswenberg. 1989. A simple and efficient non-organic procedure for the isolation of genomic DNA from blood. Nucl. Acids Res. 17(16):8390.
  11. Hurtado, A., A. L. Garcia-Perez, B. de Heredia, J. Barandika, A. Sanz-Parra, E. Berriatua and R. A Juste. 2002. Genetic susceptibility to scrapie in a population of Latsa breed sheep in the Basque country, Spain. Small Rumin. Res. 45:255-259. https://doi.org/10.1016/S0921-4488(02)00156-6
  12. Kurosaki, Y., N. Ishiguro, M. Horiuchi and M. Shinagawa. 2005. Polymorphisms of caprine PrP gene detected in Japan. J. Vet. Med. Sci. 67:321-323. https://doi.org/10.1292/jvms.67.321
  13. Papasavva-Stylianou, P., M. Kleanthous, P. Toumazos, P. Mavrikiou and P. Loucaides. 2007. Novel polymorphisms at codons 146 and 151 in the prion protein gene of Cyprus goats, and their association with natural scrapie. Vet. J. 173(2):459-462. https://doi.org/10.1016/j.tvjl.2005.09.013
  14. Prusiner, S. B. 2004. Prion biology and diseases. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
  15. Prusiner, S. B. 1998. Prions. Proc. Natl. Acad. Sci. USA. 95:13363-13383. https://doi.org/10.1073/pnas.95.23.13363
  16. Vaccari, G., M. A. Di Bari, L. Morelli, R. Nonno, B. Chiappini, G. Antonucci, S. Marcon, E. Esposito, P. Fazzi, N. Palazzini, P. Troiano, A. Petrella, G. Di Guardo and U. Agrimi. 2006. Identification of an allelic variant of the goat PrP gene associated with resistance to scrapie. J. Gen. Virol. 87(Pt 5):1395-1402. https://doi.org/10.1099/vir.0.81485-0
  17. Wopfner, F., G. Weidenho, R. Schneider, A. von Brunn, S. Gilch, T. F. Schwarz, T. Werner and H. M. Schatzl. 1999. Analysis of 27 mammalian and 9 avian PrPs reveals high conservation of flexible regions of the prion protein. J. Mol. Biol. 289:1163-1178. https://doi.org/10.1006/jmbi.1999.2831
  18. Zhang, L., N. Li, B. Fan, M. Fang and W. Xu. 2004. PRNP polymorphisms in Chinese ovine, caprine and bovine breeds. Anim. Genet. 35:457-461. https://doi.org/10.1111/j.1365-2052.2004.01204.x

Cited by

  1. Genetic TSE resistance in goats vol.7, pp.3, 2008, https://doi.org/10.2903/j.efsa.2009.995
  2. PrP gene polymorphisms in Cyprus goats and their association with resistance or susceptibility to natural scrapie vol.187, pp.2, 2011, https://doi.org/10.1016/j.tvjl.2009.10.015
  3. Potential scrapie-associated polymorphisms of the prion protein gene (PRNP) in Korean native black goats vol.9, pp.1, 2008, https://doi.org/10.1038/s41598-019-51621-y
  4. Genetic Variation in the Prion Protein Gene (PRNP) of Two Tunisian Goat Populations vol.11, pp.6, 2008, https://doi.org/10.3390/ani11061635