Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Determination of vitamin P1 by chemiluminescence method

화학발광법에 의한 비타민 P1의 정량

  • Jin, Seung Oh (Department of Chemistry, Kyungpook National University) ;
  • Lee, Sang Hak (Department of Chemistry, Kyungpook National University)
  • 진승오 (경북대학교 자연과학대학 화학과) ;
  • 이상학 (경북대학교 자연과학대학 화학과)
  • Received : 2007.10.29
  • Accepted : 2008.01.02
  • Published : 2008.02.25
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Abstract

A chemiluminescence method has been developed to determine vitamin $P_1$ in aqueous solution which is based on the enhancement of the intensity of lucigenin using lucigenin-$H_2O_2$ as chemiluminogenic system. The effects of experimental parameters such as concentrations of lucigenin, pH and concentrations of $H_2O_2$ were studied. The present method allows the determination of vitamin $P_1$ over the range $7.5{\times}10^{-6}{\sim}5.0{\times}10^{-4}mol/L$. The detection limit was $5.7{\times}10^{-7}mol/L$. The relative standard deviation was 0.75 % for 10 determinations of $7.5{\times}10^{-5}mol/L$ vitamin $P_1$. The correlation coefficient of the working curve was 0.9984 (S/N=3).

본 연구에서는 lucigenin과 $H_2O_2$에 의한 화학발광을 이용하여 vitamin $P_1$을 정량하는 방법에 대하여 연구하였다. 분석의 최적 조건을 구하기 위해서 화학발광에 영향을 미치는 $H_2O_2$의 농도, pH 그리고 lucigenin의 농도를 조사하였다. 이러한 분석 최적조건의 검정곡선에서 직선성이 성립하는 범위는 $7.5{\times}10^{-6}$에서 $5.0{\times}10^{-4}mol/L$이었고, 검정곡선에서 직선성이 성립하는 농도구간에서 구한 검출한계는 $5.7{\times}10^{-7}mol/L$이었다. 최적 vitamin $P_1$의 농도인 $7.5{\times}10^{-5}mol/L$에서의 상대표준편차는 0.75%(n=10), 상관계수(S/N=3)는 0.9984이었다.

Keywords

References

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