Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Morphine induced inhibition of the activities of accessory reproductive ducts in male rats

  • Published : 2008.03.31
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Abstract

Adult male albino rats were treated with 0.5 mg and 0.75 mg morphine/100 g body weight intraperitoneally for 30 days. All the animals were autopsied on $31^{st}$ day. Epididymis and vas deferens were dissected out, weighed and processed for histological and biochemical studies. Morphine has caused a reduction in the weight of epididymis and vas deferens in both the doses of drug treated groups. The total cholesterol content is increased while protein, DNA and RNA contents and epididymal sperm counts are decreased. The acid phosphatase content is decreased 10.12 $\pm$ 0.11 in caput, 9.26 $\pm$ 0.30 in cauda of epididymis and in vas deferens 8.14 $\pm$ 0.15 in 0.5 mg treated groups and in 0.75 mg treated rats shows 9.52 $\pm$ 0.27 in caput, 9.14 $\pm$ 0.18 in cauda of epididymis and in vas deferens 7.84 $\pm$ 0.11is decreased, whereas alkaline phosphatase is increased. The surface epithelial cell height of these ducts is reduced and secretory activity is inhibited with the disruption of epithelial cell projections. The gravimetric and histometric changes of epididymis and vas deferens may be due to non-availability of androgens in morphine treated rats.

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References

  1. Anderson K, Fuxe K, Eneroth P, Agnathi LF. (1982) Involvement of cholinergic nicotine like receptors as modulators of amine turnover in various terminal systems and prolactin, LH, FSH and ICSH secretion in castrated male rats. Acta Physiol. Scand. 116, 41-50
  2. Barraclough CA, Sawyer CH. (1955) Inhibition of the release of pituitary the epididymis of the rabbit. J. Exp. Zool. 63, 319-329
  3. Blake CA. (1974) Localization of inhibitory actions in ovulation blocking drugs on release of Luteinizing hormone in ovariectomized rats. J. Endocrinol. 95, 999-1005
  4. Blake CA. (1978) Paradoxical effects of drugs acting on the central nervous system on the preovulatory release of pituitary Luteinizing hormone in proestrous rats. J. Endocrinol. 79, 319-326
  5. Brooks DE. (1981) Effects of androgen on protein synthesis and secretion in various regions of the rat epididymis is analysed by two dimensional gel electrophoresis. Mol. Cell. Endocrinol. 29, 255-261 https://doi.org/10.1016/0303-7207(83)90016-3
  6. Bruni JF, Vanvut D, Marshall S, Meites J. (1977) Effect of naloxone, morphine and metheonine enkepaline on serum prolaction, Luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone and growth hormone. Life Sci. 21, 461-466 https://doi.org/10.1016/0024-3205(77)90528-8
  7. Connel GM, Eik-Nes KB. (1968) Testosterone production by rabbit testis slices. Steroids 12, 507-521 https://doi.org/10.1016/S0039-128X(68)80114-X
  8. Duggan AW, North RA. (1983) Electrophysiology of opioids. Pharmacol. Rev. 35, 219-282
  9. Gaddum P, Glover TD. (1965) Some relations of rabbit's spermatozoa to ligation of the epididymis. J. Reprod. Fertil. 9, 119-126
  10. Glick D. (1958) Methods of biochemical analysis. Vol. VI, pp. 10-17
  11. Hamilton DW. (1975) Structure and function of epithelium lining and ductuli efferentes, ductus epididymis and ducts deferens in the rat. In: Greep, R.O., Astood E.B. (Eds), Handbook of physiology, Section-7, Vol. 1, pp. 259-302
  12. Hanson V, Rensech E, Trygstud O, Torgeseno O, Ritzen EM, French FS. (1973) FSH stimulation of testicular androgen binding protein nature. New Biol. 246, 56-58
  13. Johnson BJ, Ewing LL. (1971) Follicle stimulating hormone and the regulation of testosterone secretion in rabbit testis. Science 173, 635-640 https://doi.org/10.1126/science.173.3997.635
  14. Kasturi MM, Manvannan B, Nazeer Ahmed, Parveen DS, Pathan KM. (1995) Changes in epididymal structural and function of albino rat treated with Azadirachta indica leaves. Indian J. Exp. Biol. 33, 725-729
  15. Kempinas WG, Lamano-Carvlho TL. (1987) A method for estimation of concentration of spermatozoa in the rat cauda epididymis. Laboratory Animals. pp. 154-156
  16. Lowry OH, Rosenbrough NJ, Farr NL, Randoll RJ. (1951) Protein measurements with folic phenol reagent. J. Biol. Chem. 193, 265-275
  17. Martin W R. (1984) Pharmacology of Opioids. Pharmacol. Rev. 35, 283-323
  18. Martin WR, Sloan JW. (1977) Neuro pharmacology and neurochemistry of subjective effects, analgesia, tolerance and dependence produced by narcotic analgesics. In: Handbook of experimental pharmacology Vol. 45/I, Drug Addiction I, Morphine, Sedative/Hypotonic and Alcohol Dependence (Martin W.R., ed.). Springer Verlag, Berlin, 43-158
  19. Ojeda SR, Urbanski HF. (1994) Puberty in rat. In: the of physiology reproductive. Second edition, Edted by knobil and Neil J.D., Raven Press, New York. Vol. II, 363-406
  20. Orgebin-Crist MC. (1969) Studies on the function of the epidermis. Biol. Reprod. Suppl 1, 155-175 https://doi.org/10.1095/biolreprod1.Supplement_1.155
  21. Orgebin-Crist MC, Danzo BJ. (1975) Endocrine control of the development and maintainance of fertilizing ability in the epididymis. In: Handbook of physiology, Section-7. (Eds. D.W. Hamilton and Greep. O). Am. physiol. Soc. Washington D.C. 5, 319-338
  22. Peters JP, Vanslyke DD. (1946) Quantitative Clinical Chemistry. Vol. I, Williams and Wilkins, Baltimore
  23. Price D, William Ashman HG. (1961) In: Young William C. (Ed.), Sex and internal secretions, Vol. I, Williams and Wilkins, Baltimore. pp. 366-448
  24. Reisine T, Pasternak. (1996) Opioid analgesic and antagonistic. In: The pharmacological basis of therapeutics (Eds. Hardman J.G., Limbird)
  25. Motinoff PB, Ruddon RW, Gilman AG. (Mc grew Hill. international edition New York) $9^{th}$ edition. 521-556
  26. Russel JA, Spears N. (1984) Morphine inhibits suckling induced oxytocin secretion in conscious lactating rats, but also disrupts maternal behaviour. J. Physiol. 346, 133-138
  27. Russel JA, Godsen RG, Humphreys EM, Cutting R, Fitzsimons N, Johnson VS, Liddle S, Scott S, Stirland JA. (1989) Interrruption of parturition in rats by morphine: A result of inhibition of oxytocin secretion. J. Endocrinol. 121, 521-536
  28. Taussaky HH, Sorr E. (1953) Microcolourometric method for the determination of inorganic phosphorus. J. Biol. Chem. 202, 675-682

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