Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Inhibitory effects of Gamichungsangbohatang on chemokines related asthma in A549 human epithelial cells

  • Jeong, Seung-Yeon (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Sung-Hun (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine College of Oriental Medicine, Kyung Hee University) ;
  • Roh, Young-Lae (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Jae-Hwan (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine College of Oriental Medicine, Kyung Hee University) ;
  • Jung, Hee-Jae (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine College of Oriental Medicine, Kyung Hee University) ;
  • Jung, Sung-Ki (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine College of Oriental Medicine, Kyung Hee University)
  • Published : 2008.02.29
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Abstract

Recently a major goal in asthma therapy is to reduce or prevent the inflammatory response of airway. Eosinophilic accumulation in the tissue is a prominent feature of allergic diseases including asthma. Production of chemokines by bronchial epithelial cells may contribute to the allergic inflammation by recruiting eosinophils. In this study we evaluated the inhibitory effect of Gamichungsangbohatang (GMCSBHT), used traditionally in treating asthma, on secretion of chemokines for eosinophils in human A549 epithelial cells. Chemokines such as eotaxin, RANTES, IL-8 were inhibited in a dose-dependent manner, but IL-16 showed no inhibition by GMCSBHT. These findings indicate that GMCSBHT might be a therapeutic value in treating asthma by suppression of chemokines secretion associated with local accumulation of eosinophils.

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References

  1. Adamko D, Odemuyiwa SO, Moqbel R. (2003) The eosinophil as a therapeutic target in asthma: beginning of the end, or end of the beginning? Curr. Opin. Pharmacol. 3, 227-232 https://doi.org/10.1016/S1471-4892(03)00040-7
  2. Alam R, York J, Boyars M, Stafford S, Grant JA, Lee J, Forsythe P, Sim T, Ida N. (1996) Increased MCP-1, RANTES, and MIP-1alpha in bronchoalveolar lavage fluid of allergic asthmatic patients. Am. J. Respir. Crit. Care Med. 153, 1398-1404
  3. Appay V, Rowland-Jones SL. (2001) RANTES: a versatile and controversial chemokine. Trends Immunol. 22, 83-87 https://doi.org/10.1016/S1471-4906(00)01812-3
  4. Arima M, Plitt J, Stellato C, Bickel C, Motojima S, Makino S, Fukuda T, Schleimer RP. (1999) Expression of interleukin-16 by human epithelial cells. Inhibition by dexamethasone. Am. J. Respir. Cell Mol. Biol. 21, 684-692
  5. Bateman ED, Hurd SS, Barnes PJ, Bousquet J, Drazen JM, Fitzgerald M, Gibson P, Ohta K, O'Byrne P, Pedersen SE, Pizzichini E, Sullivan SD, Wenzel SE, Zar HJ. (2008) Global strategy for asthma management and prevention: GINA executive summary. Eur. Respir. J. 31, 143-178 https://doi.org/10.1183/09031936.00138707
  6. Cheng G, Ueda T, Eda F, Arima M, Yoshida N, Fukuda T. (2001) A549 cells can express interleukin- 16 and stimulate eosinophil chemotaxis. Am. J. Respir. Cell Mol. Biol. 25, 212-218
  7. Choi JY, Lee JS, Jeong SY, Lee KY, Lee KK, Jung HJ, Rhee HK, Jung SK. (2004) An analysis of therapeutic effects of Gamichuongsangboha-tang in 30 asthmatics based on criteria for defiency-excess differentiating syndromes of asthma. Korean J. Orient. Int. Med. 25, 379-387
  8. Erger RA, Casale TB. (1995) Interleukin-8 is a potent mediator of eosinophil chemotaxis through endothelium and epithelium. Am. J. Physiol. 268, 117-122
  9. Ferland C, Guilbert M, Davoine F, Flamand N, Chakir J, Laviolette M. (2001) Eotaxin promotes eosinophil transmigration via the activation of the plasminogenplasmin system. J. Leukoc. Biol. 69, 772-778
  10. Fukakusa M, Bergeron C, Tulic MK, Fiset PO, Al Dewachi O, Laviolette M, Hamid Q, Chakir J. (2005) Oral corticosteroids decrease eosinophil and CC chemokine expression but increase neutrophil, IL-8, and IFN-gamma-inducible protein 10 expression in asthmatic airway mucosa. J. Allergy Clin. Immunol. 115, 280-286 https://doi.org/10.1016/j.jaci.2004.10.036
  11. Gangur V, Oppenheim JJ. (2000) Are chemokines essential or secondary participants in allergic responses? Ann. Allergy Asthma Immunol. 84, 569- 579
  12. Heo TS, Ko EJ, Jung HJ, Rhee HK, Bae HS, Jung SK. (2006) Studying of the effects of Gamichungsangbohatang water-extract and ethanol-extract on IFN-${\gamma}$, IL-4, T-bet, GATA-3, c-maf in asthma model. Korean J. Orient. Int. Med. 27, 40-54
  13. Hwang WS, Choi JY, Lee JS, Ju CY, Jung HJ, Rhee HK, Jung SK. (2003) The Steroid Sparing Effects of Cheongsangboha-tang in Asthmatic Patients. Korean J. Orient. Int. Med. 24, 1-10
  14. Jung SK, Jung HJ, Lee JS, Lee KY, Jeong SY, Rhee HK, Choi JY. (2004) The Clinical Effects of Gamichuongsangbohatang (Jiaweiqingshangbuxia-tang) extract in Asthmatic Patients Based on Severity. J. Korean Orient. Med. 25, 110-118
  15. Jung SK, Hwang WS, Ju CY, Lee JS, Cho IH, Jung HJ. (2002) Clinical effects of chuongsangboha-tang in asthmatic patients. J. Orient. Med. 23, 151-160
  16. Justice JP, Borchers MT, Crosby JR, Hines EM, Shen HH, Ochkur SI, McGarry MP, Lee NA, Lee JJ. (2003) Ablation of eosinophils leads to a reduction of allergen-induced pulmonary pathology. Am. J. Physiol. Lung Cell Mol. Physiol. 284, 169-178
  17. Kikuchi S, Nagata M, Kikuchi I, Hagiwara K, Kanazawa M. (2005) Association between neutrophilic and eosinophilic inflammation in patients with severe persistent asthma. Int. Arch. Allergy Immunol. 137, 7-11 https://doi.org/10.1159/000085425
  18. Laberge S, Pinsonneault S, Varga EM, Till SJ, Nouri- Aria K, Jacobson M, Cruikshank WW, Center DM, Hamid Q, Durham SR. (2000) Increased expression of IL-16 immunoreactivity in bronchial mucosa after segmental allergen challenge in patients with asthma. J. Allergy Clin. Immunol. 106, 293-301 https://doi.org/10.1067/mai.2000.108112
  19. Min JK, Jung HJ, Rhee HK, Jung SK. (2006) Comparison and analysis of several main components between hot water extract and alcohol extract of Gamichungsangbohatang. Korean J. Orient. Int. Med. 27, 55-71
  20. Norzila MZ, Fakes K, Henry RL, Simpson J, Gibson PG. (2000) Interleukin-8 secretion and neutrophil recruitment accompanies induced sputum eosinophil activation in children with acute asthma. Am. J. Respir. Crit. Care Med. 161, 769-774
  21. Ordonez CL, Shaughnessy TE, Matthay MA, Fahy JV. (2000) Increased neutrophil numbers and IL-8 levels in airway secretions in acute severe asthma: Clinical and biologic significance. Am. J. Respir. Crit. Care Med. 161, 1185-1190
  22. Ono SJ, Nakamura T, Miyazaki D, Ohbayashi M, Dawson M, Toda M. (2003) Chemokines: roles in leukocyte development, trafficking, and effector function. J. Allergy Clin. Immunol. 111, 1185-1199 https://doi.org/10.1067/mai.2003.1594
  23. Roh GS, Seo SW, Yeo S, Lee JM, Choi JW, Kim E, Shin Y, Cho C, Bae H, Jung SK, Kwack K. (2005) Efficacy of a traditional Korean medicine, Chung-Sang-Bo- Ha-Tang, in a murine model of chronic asthma. Int. Immunopharmacol. 5, 427-436 https://doi.org/10.1016/j.intimp.2004.09.036
  24. Rojas-Ramos E, Avalos AF, Perez-Fernandez L, Cuevas-Schacht F, Valencia-Maqueda E, Teran LM. (2003) Role of the chemokines RANTES, monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 in childhood asthma. Eur. Respir. J. 22, 310-316 https://doi.org/10.1183/09031936.03.00084802
  25. Rothenberg ME, MacLean JA, Pearlman E, Luster AD, Leder P. (1997) Targeted disruption of the chemokine eotaxin partially reduces antigeninduced tissue eosinophilia. J. Exp. Med. 185, 785-790 https://doi.org/10.1084/jem.185.4.785
  26. Rothenberg ME. (1998) Eosinophilia. N. Engl. J. Med. 338, 1592-1600 https://doi.org/10.1056/NEJM199805283382206
  27. Samson M, Labbe O, Mollereau C, Vassart G, Parmentier M. (1996) Molecular cloning and functional expression of a new human CC-chemokine receptor gene. Biochemistry 35, 3362-3367 https://doi.org/10.1021/bi952950g
  28. Shute J. (1994) Interleukin-8 is a potent eosinophil chemo-attractant. Clin. Exp. Allergy 24, 203-206 https://doi.org/10.1111/j.1365-2222.1994.tb00220.x
  29. Teran LM, Noso N, Carroll M, Davies DE, Holgate S, Schroder JM. (1996) Eosinophil recruitment following allergen challenge is associated with the release of the chemokine RANTES into asthmatic airways. J. Immunol. 157, 1806-1812
  30. Tillie-Leblond I, Hammad H, Desurmont S, Pugin J, Wallaert B, Tonnel AB, Gosset P. (2000) CC chemokines and interleukin-5 in bronchial lavage fluid from patients with status asthmaticus. Potential implication in eosinophil recruitment. Am. J. Respir. Crit. Care Med. 162, 586-592
  31. Wardlaw A. (2004) Eosinophil trafficking: new answers to old questions. Clin. Exp. Allergy 34, 676- 679 https://doi.org/10.1111/j.1365-2222.2004.1944.x
  32. Yoshimoto T, Wang CR, Yoneto T, Matsuzawa A, Cruikshank WW, Nariuchi H. (2000) Role of IL-16 in delayed-type hypersensitivity reaction. Blood 95, 2869-2874