Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Identification of to Hexapeptides that Render C2 Myoblasts the Resistant Menadione-induced Cell Death

  • Hwang, Sung-Ho (Department of Biological Science, College of Natural Sciences, Ajou University) ;
  • Kim, Min-Jeong (Department of Biological Science, College of Natural Sciences, Ajou University) ;
  • Lim, Jeong-A (Department of Biological Science, College of Natural Sciences, Ajou University) ;
  • Woo, Joo-Hong (Department of Biological Science, College of Natural Sciences, Ajou University) ;
  • Kim, Hye-Sun (Department of Biological Science, College of Natural Sciences, Ajou University)
  • Published : 2008.03.31
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Abstract

Menadione induced cell death in cultured C2 myoblasts. By screening synthetic peptide libraries composed of random sequence of hexapeptides, we identified the hexa-peptides pool of(Ala/Ile)-(Ile/Met)-Val-Ile-Asp-(Met/Ser)-$NH_2$ that protected the myoblasts against menadioneinduced cell death. Pre-incubation with the hexapeptide pool reduced the number of cells detached from culture dish substrate and increased the ratio of relative viability against menadione. In addition, the peptides strongly increased the expression of Bcl-2, an anti-apoptotic protein. These results suggest that the hexapeptides might enhance the resistance to cell death against menadione by increasing the expression of Bcl-2.

Keywords

References

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