Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protection of Codonopis pilosula Extract against Cell Death of SK-N-MC Neuroblastoma Cells Treated with $H_2O_2$

만삼(蔓蔘)의 과산화수소에 의한 SK-N-MC의 세포사에 미치는 영향

  • Lee, Seon-Goo (Department of Oriental Pathology, College of Oriental Medicine, Sangji University)
  • 이선구 (상지대학교 한의과대학 병리학교실)
  • Published : 2008.04.25
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to identify the protective effect of Codonopis pilosula extract on cell death induced by $H_2O_2$ in SK-N-MC neuroblastoma cells. We measured the antioxidant effect by DPPH radical scavenging analysis, BSA analyssis and examined the cell viability by crystal violet and cytochrome C, Bax, Bcl-2, p53, p21 by using Western blot analysis. Codonopis pilosula extract scavenged DPPH radical in a dose-dependent manner and shown direct free radical scavenging effect, suggested that Codonopis pilosula extract have antioxidant effect in vitro. Treatment of cells with hydrogen peroxide, a reactive oxygen species, was to induce cell death and pretreatment with Codonopis pilosula extract attenuated the occurrence of $H_2O_2-induced$ cell death. To elucidate the protective mechanisms of action of Codonopis pilosula extract, Western blot analyses for Bcl-2 and Bax expression and cytochrome c release were carried out. Pretreatment with Codonopis pilosula extract induced the expression of Bcl-2 and suppressed the release of cytochrome c and Bax into the cytosol, thereby arresting $H_2O_2-induced$ apoptotic cell death. Especially p21 and p53 were decreased prior to $H_2O_2$ treatment. These results suggest that Codonopis pilosula extract is associated with the cell cycle and anti-apoptotic cell death.

Keywords

References

  1. 전국한의과대학 본초학교실. 본초학. pp 533-534, 1991
  2. wyllie, A.H. Apoptosis Br J cancer 67: 205-208, 1993 https://doi.org/10.1038/bjc.1993.40
  3. Halliwell, B, Gutteridge, J.M. Protection against oxidants in biological systems: the superoxide theory of Oxygen toxicity. In free radicals in biology and medicine. 2nd Ed. Oxford, Clarendon Press. pp 86-89, 1989
  4. 채창훈, 김지연, 박해준, 안응남, 김현태. 운동유발성 Apoptosis에 미치는 ${\alpha}$-lipoic acid의 방어효과. The korean Journal of Physical Education 45(2):481-489, 2006
  5. Groswami, R., Reed, J.C. Dose ceramide play a role in neural cell apoptosis?. J. Neurosci. Res. 60: 141-149, 2000 https://doi.org/10.1002/(SICI)1097-4547(20000415)60:2<141::AID-JNR2>3.0.CO;2-5
  6. Carmody, R.J. Reactive oxygen species as mediators of photoreceptor apoptosis in vitro. Exp. Cell. Res. 248: 520-530, 1999 https://doi.org/10.1006/excr.1998.4421
  7. 박종희, 권성재, 오종영. 한약 만삼의 생약학적연구. 생약학회지 36(1):21-25, 2005
  8. 이인란. 만삼엑기스가 임파성백형병 P388에 미치는 영향. 생약학회지 11(3):104-107, 1980
  9. Chandra, J., Samali, A., Orrenius, S. Triggering and modulation of apoptosis by oxidative stress. Free Radic Biol Med 29(3-4):323-333, 2000 https://doi.org/10.1016/S0891-5849(00)00302-6
  10. Steensberg, A., Morrow, J., Toft, A.D., Bruunsgaard, H., Pedersen, B.K. Prolonged exercise, lymphocyte apoptosis and F2-isoprostanse. Eur. J. Appl. Physiol. 87: 38-42, 2002 https://doi.org/10.1007/s00421-002-0584-6
  11. Martin, G.M. Austad, S.N, Johnson, T.E. Genetic analysis of ageing: role of oxidative damage and envrionmental stresses. Nature Genet. 13: 25-34, 1996 https://doi.org/10.1038/ng0596-25
  12. 황지영, 장혜순, 유현희, 문해닮아, 전병훈, 유용욱. 갈근으로부터 추출한 $\beta$-sitosterol이 HEI-OC1 세포의 항산화효소활성에 미치는 영향. 동의생리병리학회지 21(4):884-890, 2007
  13. 박혜련. 나문희. 윤용갑. 전병훈. 안원근. K562세포의 apoptosis 유도에 미치는 저근백피의 효과. 동의생리병리학회지 18(6):1728-1732, 2004
  14. Chiarugi, V., Magnelli, L., Basi, G. Apoptosis and the cell cycle. Cell. Mol. Biol. Res. 40: 603-612, 1994
  15. Nagata, S. Apoptosis by death factor. Cell 88: 355-365, 1997 https://doi.org/10.1016/S0092-8674(00)81874-7
  16. Hattori, T., Ookawa, N., Fujita, R., Fukuchi, K. Hetero- colorectal cancer. Acta Oncologica 39(4):495-500, 2000 https://doi.org/10.1080/028418600750013410
  17. Tudor, G., Aguilera, A., Halverson, D.P., Laing, N.D., Sausville, E.A. Susceptibility to drug-induced apoptosis correlates with differential of Bad, Bcl-2 and Bcl-xL protein levels. Cell Death & Differentiation 7(6):574-586, 2000 https://doi.org/10.1038/sj.cdd.4400688
  18. 최영현. 최우영. 최병태. 이용태. 이원호. 비소화합물에 의한 A549 폐암세포의 증식억제에 관한 연구. 동의생리병리학회지 19(4):1050-1054, 2005
  19. Condorelli, G., Morisco, C., Stassi, G., Notte, A., Farina, F., Sgaramella, G., de Rienzo, A., Roncarati, R., Trimarco, B., Lembo, G. Increased cardiomyocyte apoptosis and changes in proapoptotic and antiapoptotic genes bax and bcl-2 during left ventricular adaaptations to chronic pressure overload in the rat. Circulation 99(23):3071-3078, 1999 https://doi.org/10.1161/01.CIR.99.23.3071
  20. Vander, Heiden, M.G., Thompson, C.B. Bcl-2 proteins: regulators of apoptosis or of mitochondrial homeostasis?. Nature Cell Biology 1(8):E209-216, 1999 https://doi.org/10.1038/70237
  21. Kroemer, G. The proto-oncogene Bcl-2 and its role in regulating apoptosis. Nature Medicine 3(6):614-620, 1997 https://doi.org/10.1038/nm0697-614
  22. Brandy, H.J., Gil-Gomez, G. Bax. The pro-apoptotic Bcl-2 family member, Bax. International Journal of Biochemical Cell Biology 30(6):647-650, 1998 https://doi.org/10.1016/S1357-2725(98)00006-5
  23. Lane, D.P. A death in the life of p53. Nature 362(6423): 849-852, 1993 https://doi.org/10.1038/362849a0
  24. 안원근. 노화과정에 있어서 p21 유전자의 역할. 생화학분자생물학뉴스, 한국 생화학분자생물학회 23(2):80-81, 2003