Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of the Ethanol Extract of Vitis labrusca Root on Apoptosis in Hep G2 Cells

포도근 에탄올 추출물이 Hep G2 세포의 자연사에 미치는 효과

  • Lee, Dong-Kyo (Department of Cellular Genetics, College of Sciences) ;
  • Lee, Kang-Pa (Department of Cellular Genetics, College of Sciences) ;
  • Kim, Hyuck (Cardiovascular Medical Research Center College of Korean Medicine) ;
  • Choi, Byung-Jin (Department of Pathology, College of Korean Medicne, Dongguk University) ;
  • Chang, Hae-Ryong (Department of Cellular Genetics, College of Sciences) ;
  • Park, Won-Hwan (Cardiovascular Medical Research Center College of Korean Medicine)
  • 이동교 (동국대학교 과학기술대학 세포유전학 실험실) ;
  • 이강파 (동국대학교 과학기술대학 세포유전학 실험실) ;
  • 김혁 (동국대학교 심혈관계질환 천연물개발연구센터) ;
  • 최병진 (동국대학교 한의과대학 병리학교실) ;
  • 장해룡 (동국대학교 과학기술대학 세포유전학 실험실) ;
  • 박원환 (동국대학교 심혈관계질환 천연물개발연구센터)
  • Published : 2008.04.25
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Abstract

The root of Vitis labrusca, is used as a source of health promoting drug in Korean traditional medicine. It has been reported that root of Vitis labrusca has antioxidant, anti lipid peroxidation and anti-reactive nitrogen species (RNS) activities. The aim of this study was to elucidate the molecular changes of apoptotic signaling pathways in phorbol 12-myristate 13 acetate (PMA)-induced human hepatocellular carcinoma cell line (Hep G2). The root of Vitis labrusca, ethanol extract (RVLEE) was tested for cell viability on Hep G2 cell using the MTT assay. RVLEE exhibited weak cytotoxic activity. However, treatment of Hep G2 cells with RVLEE suppressed PMA-induced cell proliferation. Also, dramatic changes of cell death signals in cellular molecules such as Chk2/Cds1, CIDE-B, CLIMP-63, Bax, Bcl-xL, C-myc, Bcl-2, Bric-5, NIP-3, TRAF2 and BAR but not CIDE-B and DR4. Futhermore, our results showed that the treatment of Hep G2 cells with 25 and $50\; {\mu}g/ml$ of RVLEE suppressed PMA-induced COX-2 gene activity. These data suggest that RVLEE have inhibitory effect of cell proliferation, induction of apoptosis and, thus, may offer therapeutic potential in Hep G2.

Keywords

References

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