Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Euphorbiae Humifusae Inhibits Egr-1, $NF{-\kappa}B$ and COX-2 Activity Stimulated by Phorbol 12-myristate-13-acetate

PMA에 의해 유도된 Egr-1, $NF{-\kappa}B$ 및 COX-2의 활성에 미치는 지금초 추출물의 영향

  • Kim, Tae-Hwan (Departments of Internal Medicine, Dongeui University College of Oriental Medicine) ;
  • Jin, Cheng-Yun (Department of Biochemistry, Dongeui University College of Oriental Medicine, Department of Biomaterial Control, Dongeui University Graduate School) ;
  • Park, Sang-Eun (Departments of Internal Medicine, Dongeui University College of Oriental Medicine) ;
  • Kim, Won-Il (Departments of Internal Medicine, Dongeui University College of Oriental Medicine) ;
  • Park, Dong-Il (Departments of Internal Medicine, Dongeui University College of Oriental Medicine) ;
  • Kim, Gi-Young (Faculty of Applied Marine Science, Cheju National University) ;
  • Kim, Nam-Deuk (Division of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Hong, Sang-Hoon (Departments of Internal Medicine, Dongeui University College of Oriental Medicine) ;
  • Choi, Yung-Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine, Department of Biomaterial Control, Dongeui University Graduate School)
  • 김태환 (동의대학교 한의과대학 내과학교실) ;
  • 김성윤 (생화학교실 및 대학원 바이오물질제어학과) ;
  • 박상은 (동의대학교 한의과대학 내과학교실) ;
  • 김원일 (동의대학교 한의과대학 내과학교실) ;
  • 박동일 (동의대학교 한의과대학 내과학교실) ;
  • 김기영 (제주대학교 해양과학대학) ;
  • 김남득 (부산대학교 약학대학 약학과) ;
  • 홍상훈 (동의대학교 한의과대학 내과학교실) ;
  • 최영현 (생화학교실 및 대학원 바이오물질제어학과)
  • Published : 2008.04.25
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Abstract

Pro-inflammatory mediators, such as prostaglandin $E_2$ (PGE2), nitric oxide (NO), and cyclooxygenases-2 (COX-2), play pivotal roles in normal as well as transformed cells. Previous studies have shown that Euphorbiae humifusae Wind exhibits anti-proliferative and antioxidant activities. However, the it's anti-inflammatory properties are unclear. In this study, we examine the effects of water extract of E. humifusae (WEEH) on the expression of COX-2 and the production of $PGE_2$ in human lymphatic U937 cells. Treatment of phorbol 12-myristate-13-acetate (PMA) significantly induced COX-2 expression and $PGE_2$ production in U937 cells. However, pretreatment WEEH markedly inhibited the PMA-induced COX-2 expression and $PGE_2$ production in a dose-dependent manner. Moreover, WEEH prevented the elevated early growth response gene-1 (Egr-1) expression and nuclear factor-kappaB ($NF{-\kappa}B\; p65$) nuclear translocation stimulated by PMA treatment. Taken together, the present data indicate that WEEH exhibits anti-inflammatory properties by suppressing the transcription of pro-inflammatory cytokine genes through the $NF{-\kappa}B$ and Egr-1 signaling pathway.

Keywords

References

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