Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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The Effects of Electrolyzed Reduced Water on Blood and Organ Tissues of Mice

전해환원수 음용이 마우스의 혈액과 장기조직에 미치는 영향

  • Jung, Han-Suk (Department of Chiropractic Rehabilitation, Graduate School of Health Promotion, Hanseo University) ;
  • Kim, Dong-Heui (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Yoon, Yang-Suk (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Teng, Yung-Chien (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Chang, Byung-Soo (Department of Cosmetology, Hanseo University) ;
  • Lee, Kyu-Jae (Institute of Lifelong Health, Wonju College of Medicine, Yonsei University)
  • 정한석 (한서대학교 건강증진대학원 수안재활복지학과) ;
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 윤양숙 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 장병수 (한서대학교 보건학부 피부미용학과) ;
  • 이규재 (연세대학교 원주의과대학 평생건강사업단)
  • Published : 2008.12.31
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Abstract

The main purpose of this study is to investigate the effects of electrolyzed reduced water (ERW) on blood components and electrolytes, as well as elected tissues on mice. The mice were supplied either tap water (control group) and ERW (experimental group) for two months. There were no significant different between two groups anatomically and physiologically. In the blood electrolyte study, the experimental group had less Na and BUN compared to the control group. In the blood component study, the experimental group had less neutrophiles and the control group had more lymphocytes. In histological study, no tissue changes were noticed in various organs, including the stomach, small intestine, heart, and liver tissues. In conclusion, ERW has no noticable side affects on blood and organ tissues, and might be safe to a living body.

본 연구는 전해환원수 음용에 대한 안전성을 조사하기 위하여 실험동물을 이용하여 사망률 확인, 체중 비교, 혈액의 생화학적 변화, 염증, 면역세포의 비교, 내부 장기의 형태적 변화, 장기의 무게 변화 및 조직학적 검사를 통하여 전해환원수가 생체에 미치는 영향을 확인한 결과 다음과 같은 결론을 얻었다. 전해환원수를 급이한 실험군에서 사망, 행동 및 외형 이상 유무 관찰 결과 아무런 증상이 나타나지 않았으며, 체중은 감소하는 경향을 보였다. 실험군에서 혈액의 생화학적요소인 $K^+$, $Cl^-$, $Ca^{2+}$, GPT, GOT, CRE 및 ALP에서는 유의한 수준의 변화가 관찰되지 않았으나 $Na^+$와 BUN에서는 실험군이 대조군에 비해 더 감소되었다. 혈액 내 면역세포를 비교한 결과 호염기구, 호산성구 및 단핵구 등은 큰 차이가 없으며, 실험군에서 호중구는 감소하였고, 림프구는 증가하는 경향을 보였다. 또한 위, 소장, 심장 및 간을 조직학적으로 관찰한 결과 모든 장기에서 병리적 이상 소견은 관찰되지 않았다. 따라서 본 연구를 통하여 전해환원수는 생체에서 큰 문제를 유발시키지 않는 안전한 물임을 알 수 있었고 국내에서 전해환원수에 대한 연구가 거의 없는 실정이기 때문에 전해환원수의 안전성을 입증하기 위한 기초자료 마련 및 제공에 의의가 있다고 할 수 있다.

Keywords

References

  1. Cai H, Harrison DG: Endothelial dysfunction in cardiovascular diseases; the role of oxidant stress. Circ Res 87 : 840-844, 2000 https://doi.org/10.1161/01.RES.87.10.840
  2. Chen K, Thomas SR, Keaney JF Jr: Beyond LDL oxidation. ROS in vascular signal transduction. Free Radic Biol Med 35 : 117-132, 2003 https://doi.org/10.1016/S0891-5849(03)00239-9
  3. Eid HM, Lyberg T, Larsen J, Arnesen H, Seljeflot I: Reactive oxygen species generation by leukocytes in populations at risk for atherosclerotic disease. Scand J Clin Lab Invest 62 : 431-439, 2002 https://doi.org/10.1080/00365510260389985
  4. Feig DI, Reid TM, Loeb LA: Reactive oxygen species in tumorigenesis. Cancer Res 54 : 1890-1894, 1994
  5. Hanaoka K: Antioxident effects of reduced water produced by electrolysis of sodium chloride solution. J Appl Electrochem 31 : 1307-1313, 2001 https://doi.org/10.1023/A:1013825009701
  6. Huang KC, Yang CC, Lee KT, Chien CT: Reduced hemodialysisinduced oxidative stress in end-stage renal disease patients by Electrolysed reduced water. Kidney Int 64(2) : 704-714, 2003 https://doi.org/10.1046/j.1523-1755.2003.00118.x
  7. Jin D, Ryu SH, Kim HW, Yang EJ, Lim SJ, Ryang YS, Chung CH, Park SK, Lee KJ: Anti-diabetic effect of alkaline-reduced water on OLETF rats. Biosci Biotechnol Biochem 70(1) : 31-37, 2006 https://doi.org/10.1271/bbb.70.31
  8. Kagawa Y, Cha SH, Hasegawa K, Hamamoto T, Endo H: Regulation of energy metabolism in human cells in aging and diabetes: FoF (1), mtDNA, UCP, and ROS. Bionchem Biophys Res Commun 26 : 662-676, 1999
  9. Kim DH, Deung YC, Kim D, Huang XZ, Qi XF, Kim KY, Lee KJ: Effects of Electrolyzed alkaline reduced water on Echinostoma hortense infection and immune response in C57BL/6 Mice. Korean J Microscopy 38(1) : 11-19, 2008. (Korean)
  10. Kim JM, Kazuhito Y: Effects of alkaline ionized water on spontaneously diabetic GK-rats fed sucrose. Korean J Lab Anim Sci 13(2) : 187-190, 1997
  11. Reid TM, Loeb LA: Mutagenic specificity of oxygen radicals produced by human leukemia cells. Cancer Res 52 : 1082-1086, 1992
  12. Sedeek MH, Llinas MT, Drummond H, Fortepiani L, Abram SR, Alexander BT, Reckelhoff JF, Granger JP: Role of reactive oxygen species in endothelin-induced hypertension. Hypertension 42 : 806-810, 2003 https://doi.org/10.1161/01.HYP.0000084372.91932.BA
  13. Shirahata S, Kabayama S, Nakano M, Miura T, Kusumoto K, Gotoh M, Hayashi H, Otsubo K, Morisawa S, Katakura Y: Electrolysed-reduced water scavenges active oxygen species and protects DNA from oxidative damage. Biochem Biophysic Res Commun 234 : 269-274, 1997 https://doi.org/10.1006/bbrc.1997.6622
  14. Watanabe T: Influence of alkaline ionized water on rat erythrocyte hexokinase activity and myocardium. J Toxicol Sci 22(2) : 141-152, 1997 https://doi.org/10.2131/jts.22.2_141
  15. Vorobjeva NV: Selective stimulation of the growth of anaerobic microflora in the human intestinal tract by electrolyzed reducing water. Midical Hypothesies 64 : 543-546, 2005 https://doi.org/10.1016/j.mehy.2004.07.038
  16. http://www.kfda.go.kr/open_content/news/notice_view.php?menucode=103001001&seq=842: The improvement plan of alkaline ionizer, Korea Food & Drug Administration, 2007