Studies of Anti-inflammation of Liriopis Tuber to Autoimmunune Diabetes in NOD Mice

NOD 당뇨병 생쥐에 미치는 맥문동의 항염증 효과

  • Roh, Seong-Soo (Department of Herbology, College of Oriental Medicine, Daegu Haany University) ;
  • Choi, Hak-Joo (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Kim, Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Seo, Young-Bae (Department of Herbology, College of Oriental Medicine, Daejeon University)
  • 노성수 (대구한의대 한의과대학 본초학교실) ;
  • 최학주 (대전대학교 한의과대학 병리학교실) ;
  • 김동희 (대전대학교 한의과대학 병리학교실) ;
  • 서영배 (대전대학교 한의과대학 본초학교실)
  • Published : 2008.08.25

Abstract

Bone is a dynamic tissue that is regulated by the balance between bone-resorbing osteoclasts and bone-forming osteoblasts. Curcumin isolated from Kang-hwang (Turmeric) is widely used as a foodstuff, cosmetic, and medicine. However, the effect of curcumin isolated from Kang-hwang in osteoclast differentiation remains unknown. In this study, we sought to examine the role of curcumin in osteoclast differentiation. Here we show that curcumin greatly inhibited RANKL-mediated osteoclast differentiation in osteoclast precursors without cytotoxicity. RANKL induced the phosphorylation of p38 and JNK mitogen-activated protein kinase (MAPK) and mediated $I-{\kappa}B$ degradation in bone marrow macrophages (BMMs). However, RANKL-mediated p38 MAPK phosphorylation was inhibited by the addition of curcumin. Curcumin inhibited the mRNA expression of TRAP, c-Fos, and NFATc1 in BMMs treated with RANKL. Furthermore, the protein expression of c-Fos and NFATc1 induced by RANKL was suppressed by curcumin treatment. Taken together, our results suggest that curcumin may have a potential therapeutic role in bone-related diseases such as osteoporosis by inhibiting osteoclast differentiation.

Keywords

References

  1. Makino, S., Kunimoto, K., Muraoka, Y., Mizushima, Y. and Tochino, Y. Breeding of non-obese, diadetic strain of mice. Exp. Anim. 29: 1, 1980 https://doi.org/10.1538/expanim1978.29.1_1
  2. Fujita, H., Yuri, R., Kusumoto, Y., Senzawa, Y., Makino, S. and Tochio, Y. Lymphocytic insulitis in a NOD strain of mice : an immunohistochemical and electron microscope investigation. Biomed. Res. 3: 429, 1982 https://doi.org/10.2220/biomedres.3.429
  3. Miyazaki, A., Hanafusa, T., Yamada, K., Miyakawa, J., Fujino-Kurihara, H., Nakajima, H., Konaka, K. and Tarui, S. Predominance of T lymphocytes in pacreatic islets and spleen of pre-diabetic non-obese diadetic (NOD) mice : a longitudinal study. Clin. Exp. Immunol. 60: 622, 1985
  4. Lee, K.U., Amano, K., Yoon, J.W. Evidence for initial involvement of marcropharge in development of insulitis in NOD mice. Diabetes. 37: 989, 1988 https://doi.org/10.2337/diabetes.37.7.989
  5. Mori, Y., Suko, M., Okudaria, H., Matsuba, A., Sasaki, A., Nishimura, M., Shida, T., Tanase, T. and Ikeda, Y. The adoptive transfer of spleen cells from cyclosporin treated NOD mice inhibits the development of insulintis in young NOD mice. J. Japan Dia. Soc. 30: 113, 1987
  6. Reznik, V.M., Durhan, B.L., Jones, K.L. and Mendoza, S.A. Changes in facial appearance during cyclosporin treatment. Lancet. 1: 1405, 1987
  7. 전국한의과대학공동교재편찬위원회. 본초학. 영림사, 서울, pp 644-655, 2007
  8. 전현정. 糖尿病의 原因과 症狀에 대한 東西洋의 病機論的 接近 및 東, 西洋, 代替醫學的 治療硏究. 대한한의정보학회지11(1):1-25, 2005
  9. 白政翰, 金鍾大, 姜錫峯, 消渴의 原因과 分類에 關한 文獻的 考察, 동서의학. 20(3):41-59, 1995
  10. 오양석. NOD Mouse에 관하여. 한국실험동물학회지 8(1):1- 11, 1992
  11. Vitaly Ablamunits, Dana Elias, Tamara Reshef and Irun R. Cohen. Islet T cells secreting IFN-${\gamma}$ in NOD mouse Diabetes: Arrest by p277 peptide treatment. J. of Autoimmu. 11: 73-81, 1998 https://doi.org/10.1006/jaut.1997.0177
  12. Helga Rothe, Tutsomu Hibino, Yasuhiro Itoh, Hubert Kolb and Stephan Martin. Systemic production of IFN-${\gamma}$ inducing factor versus local IFN-${\gamma}$ expression involved in the denelopment of Th1 insulitis in NOD mice. J. of Autoimmu. 10: 251-256, 1997 https://doi.org/10.1006/jaut.1997.0135