Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Inhibitory Effect of Taraxci Herba Methanol Extract on Pro-inflammatory Mediator in Lipopolysaccharide;Activated Raw 264.7 cells

  • Jo, Mi-Jeong (College of Oriental Medicine, BK21 Team, Daegu Haany University) ;
  • Chu, Yan-Hui (Department of Biochemistry, Key Laboratory of Heilongjiang Province, Mudanjiang Medical University) ;
  • Back, Young-Doo (College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Byung-Wook (College of Oriental Medicine, Daegu Haany University) ;
  • Shin, Soon-Shik (College of Oriental Medicine, Dongeui University) ;
  • Kwon, Young-Kyu (College of Oriental Medicine, BK21 Team, Daegu Haany University) ;
  • Kim, Sang-Chan (College of Oriental Medicine, BK21 Team, Daegu Haany University)
  • Published : 2008.08.25
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Abstract

Taraxci Herba (TH; Pogongyoung in Korean) has been used in traditional oriental medicine for the treatment of various ailments. The biological activity of this plant is not yet evaluated systematically. This study was conducted to evaluate the inhibitory effects of TH on the production of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-activated Raw264.7 cells. The aim of the present work is to investigate a potential anti-inflammatory activity of TH. The Raw264.7 cells were cultured in DMEM medium for 24 h. After serum starvation for 12 h, the cells were treated with TH for 1 h, followed by stimulating NO production with LPS ($2{\mu}g/ml$). As result of this study, TH inhibited the levels of NO, PGE2, $TNF-{\alpha}$, IL-6 and $IL-1{\beta}$, and the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) activated by LPS. These inhibitory effects were mediated though the inhibition of phosphorylation of inhibitory kappa B ($I{\kappa}B$). These findings showed that TH could have some anti-inflammatory effects.

Keywords

References

  1. Gruenwald, J., Brendler, T. PDR for Herbal Medicines (4th Edition). Thomson Healthcare. pp 252-254. 2004
  2. Yun, S.I., Cho, H.R. and Choi, H.S. Anticoagulant from Taraxacum platycarpum. Biosci Biotechnol Biochem. 66: 1859-1864, 2002 https://doi.org/10.1271/bbb.66.1859
  3. Chun, S.C., Jee, S.Y., Lee, S.G., Park, S.J., Lee, J.R. and Kim, S.C. Anti-Inflammatory Activity of the Methanol Extract of Moutan Cortex in LPS-Activated Raw264.7 Cells. Evid Based Complement Alternat Med. 4(3):327-333, 2007 https://doi.org/10.1093/ecam/nel093
  4. Corriveau, C.C. and Danner, R.L. Endotoxin as a therapeutic target in septic shock. Infect Agents Dis. 2: 35-43, 1993
  5. Watson, W.H., Zhao, Y. and Chawla, R.K. S-adenosylmethionine attenuates the lipopolysaccharide-induced expression of the gene for the tumour necrosis factor-a. Biochem J. 342: 21-25, 1999 https://doi.org/10.1042/0264-6021:3420021
  6. Puskas, B.L., Menke, N.E., Huie, P., Song, Y., Ecklund, K., Trindade, M.C., Smith, R.L. and Goodman, S.B. Expression of nitric oxide, peroxynitrite, and apoptosis in loose total hip replacements. J. Biomed Mater Res. A. 66: 541-549, 2003
  7. Alderton, W.K., Cooper, C.E. and Knowles, R.G. Nitric oxide synthases: structure, function and inhibition. Biochem. J. 357: 593-615, 2001 https://doi.org/10.1042/0264-6021:3570593
  8. Park, E.K., Shin, Y.W., Lee, H.U., Kim, S.S., Lee, Y.C., Lee, B.Y. and Kim, D.H. Inhibitory effect of ginsenoside Rb1 and compound K on NO and prostaglandin E2 biosyntheses of Raw264.7 cells induced by lipopolysaccharide. Biol. Pharm. Bull. 28(4):652-656, 2005 https://doi.org/10.1248/bpb.28.652
  9. Beutler, B. and Cerami, A. The biology of cachectin/TNF-$\alpha$ primary mediator of the host response. Annu Rev Immunol. 7: 625-655, 1989 https://doi.org/10.1146/annurev.iy.07.040189.003205
  10. Lee, A.K., Sung, S.H., Kim, Y.C. and Kim, S.G. Inhibition of lipopolysaccharide- inducible nitric oxide synthase, TNF-alpha and COX-2 expression by sauchinone effects on I-kappaBalpha phosphorylation, C/EBP and AP-1 activation. Br J Pharmacol. 139: 11-20, 2003 https://doi.org/10.1038/sj.bjp.0705231
  11. Roshak, A.K., Jackson, J.R., Mcgough, K., Chabot-Fletcher, M., Mochan, E. and Marshall, L.A. Manipulation of distinct NF-kappaB proteins alters interleukin-1beta-induced human rheumatoid synovial fibroblast prostaglandin E2 formation. J Biol Chem. 271: 31496-31501, 1996 https://doi.org/10.1074/jbc.271.49.31496
  12. Yoshimura, A. Signal transduction of inflammatory cytokines and tumor development. Cancer Sci. 97: 439-447, 2006 https://doi.org/10.1111/j.1349-7006.2006.00197.x
  13. Mitchell, J.A., Belvisi, M.G., Akarasereenont, P., Robbins, R.A., Kwon, O.J., Croxtall, J., Barnes, P.J. and Vane, J.R. Induction of cyclooxygenase-2 by cytokines in human pulmonary epithelial cells: regulation by dexamethasone. Br. J. Pharmacol. 113: 1008-1014, 1994 https://doi.org/10.1111/j.1476-5381.1994.tb17093.x
  14. Kim, Y.W., Zhao, R.J., Park, S.J., Lee, J.R., Cho, I.J., Yang, C.H., Kim, S.G. and Kim, S.C. Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-kB-dependent iNOS and proinflammatory cytokines production. Br J Pharmacol. pp 165-173, 2008
  15. Kubes, P. and McCaferty, D.M. Nitric oxide and intestinal inflammation. Am. J. Med. 109: 150-158, 2000 https://doi.org/10.1016/S0002-9343(00)00480-0
  16. Palmer, R.M., Ashton, D.S. and Moncada, S. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature. 333: 664-666, 1998 https://doi.org/10.1038/333664a0
  17. Kim, N.D., Kim, E.M., Kang, K.W., Cho, M.K., Choi, S.Y. and Kim, S.G. Ginsenoide Rg3 inhibits phenylephrine-induced vascular contraction though induction of nitric oxide synthase. Br. J. Pharmacol. 140: 661-670, 2003 https://doi.org/10.1038/sj.bjp.0705490
  18. MacMicking, J., Xie, Q.W. and Nathan, C. Nitric oxide and macrophage fuction. Annu. Rev. Immunol. 15: 323-350, 1997 https://doi.org/10.1146/annurev.immunol.15.1.323
  19. Zhai, Z., Haney, D., Wu, L., Solco, A., Murphy, P.A., Wurtele, E.S., Kohut, M.L. and Cunnick, J.E. Alcohol extracts of Echinacea inhibit production of nitric oxide and tumor necrosis factor-alpha by macrophages in vitro. Food Agric Immunol. 18: 221-236, 2007 https://doi.org/10.1080/09540100701797363
  20. Bogdan, C. Nitric oxide and the immune response. Nat Immunol. 2(10):907-916, 2001 https://doi.org/10.1038/ni1001-907
  21. Miljkovic, D. and Trajkovic, V. Inducible nitric oxide synthase activation by interleukin-17. Cytokine Growth Factor Rev. 15: 21-32, 2004 https://doi.org/10.1016/j.cytogfr.2003.10.003
  22. Castranova, V. Role of nitric oxide in the progression of pneumoconiosis. Biochemistry. 69: 32-37, 2004 https://doi.org/10.1023/B:BIRY.0000016348.34175.53
  23. Ravi, R. and Bedi, A. NF-kappa B in cancer-a friend turned foe. Drug Resist Updat. 7: 53-67, 2004 https://doi.org/10.1016/j.drup.2004.01.003
  24. Steinbach, G., Lynch, P.M., Phillips, R.K., Wallace, M.H., Hawk, E., Gordon, G.B., Wakabayashi, N., Saunders, B., Shen, Y., Fujimura, T., Su, L.K. and Levin, B. The effect of celecoxib, a cyclooxygenase-2 inhibitor, in familial adenomatous polyposis. Engl J Med. 342: 1946-1952, 2000 https://doi.org/10.1056/NEJM200006293422603
  25. West, M.A., Seatter, S.C., Bellingham, J. and Clair, L. Mechanisms of reprogrammed macrophage endotoxin signal transduction after lipopolysaccharide pretreatment. Surgery. 118(2):220-228, 1995 https://doi.org/10.1016/S0039-6060(05)80327-7
  26. Jean-Baptiste, E. Cellular mechanisms in sepsis. J Intensive Care Med. 22(2):63-72, 2007 https://doi.org/10.1177/0885066606297123
  27. Takabayashi, T., Shimizu, S., Clark, B.D., Beinborn, M., Burke, J.F. and Gelfand, J.A. Interleukin-1 upregulates anaphylatoxin receptors on mononuclear cells. Surgery. 135(5):544-554, 2004 https://doi.org/10.1016/j.surg.2003.09.010
  28. Hedger, M.P. and Meinhardt, A. Cytokines and the immune-testicular axis. J Reprod Immunol. 58: 1-26, 2003 https://doi.org/10.1016/S0165-0378(02)00060-8
  29. Shishodia, S. and Aggarwal, B.B. Nuclear factor-kappaB: a friend or a foe in cancer?. Biochem Pharmacol. 68: 1071-1080, 2004 https://doi.org/10.1016/j.bcp.2004.04.026
  30. Chen, F., Castranova, V. and Shi, X. New insight into the role of nuclear factor-kappaB in cell growth regulation. Am J Pathol. 159: 387-397, 2001 https://doi.org/10.1016/S0002-9440(10)61708-7