동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제22권3호
  • /
  • Pages.600-606
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  • 2008
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

PMA와 A23187로 자극된 RBL-2H3 세포에 미치는 황련해독탕(黃連解毒湯)의 영향

Anti-allergic Inflammatory Effects of Hwangyounhaedog-tang on PMA Plus A23187-stimulated RBL-2H3 Cells

  • 백상인 (동의대학교 한의과대학 한의학과) ;
  • 배종훈 (동의대학교 한의과대학 한의학과) ;
  • 김진영 (동의대학교 한의과대학 한의학과) ;
  • 심성흠 (동의대학교 한의과대학 한의학과) ;
  • 강경화 (동의대학교 한의과대학 한의학과) ;
  • 이승연 (동의대학교 한의과대학 한의학과) ;
  • 감철우 (동의대학교 한의과대학 한의학과) ;
  • 박동일 (동의대학교 한의과대학 한의학과)
  • Baek, Sang-In (Department of Oriental Medicine, Graduate School Dong-Eui University) ;
  • Bae, Jong-Hoon (Department of Oriental Medicine, Graduate School Dong-Eui University) ;
  • Kim, Jin-Young (Department of Oriental Medicine, Graduate School Dong-Eui University) ;
  • Sim, Sung-Heum (Department of Oriental Medicine, Graduate School Dong-Eui University) ;
  • Kang, Kyung-Hwa (Department of Oriental Medicine, Graduate School Dong-Eui University) ;
  • Lee, Seung-Yeon (Department of Oriental Medicine, Graduate School Dong-Eui University) ;
  • Kam, Cheol-Woo (Department of Oriental Medicine, Graduate School Dong-Eui University) ;
  • Park, Dong-Il (Department of Oriental Medicine, Graduate School Dong-Eui University)
  • 발행 : 2008.06.25
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초록

The purpose of this study was to investigate Anti-allergic Inflammatory Effects of Hwangyounhaedog-tang on PMA plus A23187-stimulated RBL-2H3 cells. We examined MTT assay, ${\beta}$-hexosaminidase activity, IL-4 and TNF-${\alpha}$ from RBL-2H3 after pre-treatment with HHT. In addition, we investigated expression of COX-1 and COX-2 mRNA using RT-PCR study. The results are as follows: All the concentrations of HHT from 1 to 4 mg/ml didn't have an effect on cell viability. In RBL-2H3 cells, ${\beta}$-hexosaminidase release, IL-4 and TNF-${\alpha}$ were significantly reduced by treated all concentrations of HHT. COX-2 on mRNA expression was reduced at treatment 2mg of HHT prior to stimulating by A23187. These results indicate that HHT has inhibition effects on allergy in this study.

키워드

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