The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Characterization of Ionic Currents in Human Neural Stem Cells

  • Lim, Chae-Gil (Department of Physiology, College of Medicine, Dankook University, Department of Physical Therapy, Gachon University of Medicine and Science Zucheon) ;
  • Kim, Sung-Soo (Department of Anatomy, Ajou University School of Medicine) ;
  • SuhKim, Hae-Young (Department of Anatomy, Ajou University School of Medicine) ;
  • Lee, Young-Don (Department of Anatomy, Ajou University School of Medicine) ;
  • Ahn, Seung-Cheol (Department of Physiology, College of Medicine, Dankook University)
  • Published : 2008.08.31
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Abstract

The profile of membrane currents was investigated in differentiated neuronal cells derived from human neural stem cells (hNSCs) that were obtained from aborted fetal cortex. Whole-cell voltage clamp recording revealed at least 4 different currents: a tetrodotoxin (TTX)-sensitive $Na^+$ current, a hyperpolarization-activated inward current, and A-type and delayed rectifier-type $K^+$ outward currents. Both types of $K^+$ outward currents were blocked by either 5 mM tetraethylammonium (TEA) or 5 mM 4-aminopyridine (4-AP). The hyperpolarization-activated current resembled the classical $K^+$ inward current in that it exhibited a voltage-dependent block in the presence of external $Ba^{2+}$ (30 ${\mu}$M) or $Cs^+$ (3${\mu}$M). However, the reversal potentials did not match well with the predicted $K^+$ equilibrium potentials, suggesting that it was not a classical $K^+$ inward rectifier current. The other $Na^+$ inward current resembled the classical $Na^+$ current observed in pharmacological studies. The expression of these channels may contribute to generation and repolarization of action potential and might be regarded as functional markers for hNSCs-derived neurons.

Keywords

References

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