The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Sorting Nexin 17 Interacts Directly with Kinesin Superfamily KIF1B${\beta}$ Protein

  • Seog, Dae-Hyun (Departments of Biochemistry, National Research Laboratory for Mitochondrial Signaling, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Han, Jin (Departments of Physiology, National Research Laboratory for Mitochondrial Signaling, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University)
  • Published : 2008.08.31
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Abstract

KIF1B${\beta}$ is a member of the Kinesin superfamily proteins (KIFs), which are microtubule-dependent molecular motors that are involved in various intracellular organellar transport processes. KIF1B${\beta}$ is not restricted to neuronal systems, however, is widely expressed in other tissues, even though the function of KIF1B${\beta}$ is still unclear. To elucidate the KIF1B${\beta}$-binding proteins in non-neuronal cells, we used the yeast two-hybrid system, and found a specific interaction of KIF1B${\beta}$ and the sorting nexin (SNX) 17. The C-terminal region of SNX17 is required for the binding with KIF1B${\beta}$. SNX17 protein bound to the specific region of KIF1Bf3 (813-916. aa), but not to other kinesin family members. In addition, this specific interaction was also observed in the Glutathione S-transferase pull-down assay. An antibody to SNX17 specifically co-immunoprecipitated KIF1B${\beta}$ associated with SNX17 from mouse brain extracts. These results suggest that SNX17 might be involved in the KIF1B${\beta}$-mediated transport as a KIF1B${\beta}$ adaptor protein.

Keywords

References

  1. Aizawa H, Sekine Y, Takemura R, Zhang Z, Nangaku M, Hirokawa N. Kinesin family in murine central nervous system. J Cell Biol 119: 1287-1296, 1992
  2. Bunn RC, Jensen MA, Reed BC. Protein interactions with the glucose transporter binding protein GLUT1CBP that provide a link between GLUT1 and the cytoskeleton. Mol Biol Cell 10: 819-832, 1999 https://doi.org/10.1091/mbc.10.4.819
  3. Burden JJ, Sun XM, Garcia AB, Soutar AK. Sorting motifs in the intracellular domain of the low density lipoprotein receptor interact with a novel domain of sorting nexin-17. J Biol Chem 279: 16237-16245, 2004 https://doi.org/10.1074/jbc.M313689200
  4. Chishti AH, Kim AC, Marfatia SM, Lutchman M, Hanspal M, Jindal H, Liu SC, Low PS, Rouleau GA, Mohandas N, Chasis JA, Conboy JG, Gascard P, Takakuwa Y, Huang SC, Benz EJ Jr, Bretscher A, Fehon RG, Gusella JF, Ramesh V, Solomon F, Marchesi VT, Tsukita S, Tsukita S, Arpin M, Louvard D, Tonks NK, Anderson JM, Fanning AS, Bryant PJ, Woods DF, Hoover KB. The FERM domain: aunique module involved in the linkage of cytoplasmic proteins to the membrane. Trends Biochem Sci 23: 281-282, 1998 https://doi.org/10.1016/S0968-0004(98)01237-7
  5. Dorner C, Ullrich A, Haring HU, Lammers R. The kinesin-like motor protein KIF1C occurs in intact cells as a dimer and associates with proteins of the 14-3-3 family. J Biol Chem 274: 33654-33660, 1999 https://doi.org/10.1074/jbc.274.47.33654
  6. Florian V, Schluter T, Bohnensack R. A new member of the sorting nexin family interacts with the C-terminus of P-selectin. Biochem Biophys Res Commun 281: 1045-1050, 2001 https://doi.org/10.1006/bbrc.2001.4467
  7. Goldstein LS. Kinesin molecular motors: transport pathways, receptors, and human disease. Proc Natl Acad Sci U S A 98: 6999-7003, 2001.
  8. Hirokawa N. Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science 279: 519-526, 1998 https://doi.org/10.1126/science.279.5350.519
  9. Kanai Y, Okada Y, Tanaka Y, Harada A, Terada S, Hirokawa N. KIF5C, A novel neuronal kinesin enriched in motor neurons. J Neurosci 20: 6374-6384, 2000 https://doi.org/10.1523/JNEUROSCI.20-17-06374.2000
  10. Karcher RL, Deacon SW, Gelfand VI. Motor-cargo interactions: the key to transport specificity. Trends Cell Biol 12: 21-27, 2002 https://doi.org/10.1016/S0962-8924(01)02184-5
  11. Kim SJ, Lee CH, Park HY, Yea SS, Jang WH, Lee SK, Park YH, Jung YW, Seog DH. Kinesin superfamily KIF1B alpha protein binds to the PDZ domain of MALS-3. The Korean Journal of Anatomy 39: 375-382, 2006
  12. Knauth P, Schluter T, Czubayko M, Kirsch C, Florian V, Schreckenberger S, Hahn H, Bohnensack R. Functions of sorting nexin 17 domains and recognition motif for P-selectin trafficking. J Mol Biol 347: 813-825, 2005 https://doi.org/10.1016/j.jmb.2005.02.004
  13. Kondo S, Sato-Yoshitake R, Noda Y, Aizawa H, Nakata T, Matsuura Y, Hirokawa N. KIF3A is a new microtubule-based anterograde motor in the nerve axon. J Cell Biol 125: 1095-1107, 1994 https://doi.org/10.1083/jcb.125.5.1095
  14. Miki H, Setou M, Kaneshiro K Hirokawa N. All kinesin superfamily protein, KIF, genes in mouse and human. Proc Natl Acad Sci USA 98: 7004-7011, 2001
  15. Mok H, Shin H, Kim S, Lee JR, Yoon J, Kim E. Association of the kinesin superfamily motor protein KIF1Balpha with postsynaptic density-95 (PSD-95), synapse-associated protein- 97, and synaptic scaffolding molecule PSD-95/discs large/zona occludens-1 proteins. J Neurosci 22: 5253-5258, 2002 https://doi.org/10.1523/JNEUROSCI.22-13-05253.2002
  16. Nakagawa T, Setou M, Seog D, Ogasawara K, Dohmae N, Takio K, Hirokawa N. A novel motor, KIF13A, transports mannose-6- phosphate receptor to plasma membrane through direct interaction with AP-1 complex. Cell 103: 569-581, 2000 https://doi.org/10.1016/S0092-8674(00)00161-6
  17. Nangaku M, Sato-Yoshitake R, Okada Y, Noda Y, Takemura R, Yamazaki H, Hirokawa N. KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria. Cell 79: 1209-1220, 1994 https://doi.org/10.1016/0092-8674(94)90012-4
  18. Okada Y, Higuchi H, Hirokawa N. Processivity of the single-headed kinesin KIF1A through biased binding to tubulin. Nature 424: 574-577, 2003 https://doi.org/10.1038/nature01804
  19. Okada Y, Hirokawa N. A processive single-headed motor: kinesin superfamily protein KIF1A. Science 283: 1152-1157, 1999 https://doi.org/10.1126/science.283.5405.1152
  20. Okada Y, Yamazaki H, Sekine-Aizawa Y, Hirokawa N. The neuron- specific kinesin superfamily protein KIF1A is a unique monomeric motor for anterograde axonal transport of synaptic vesicle precursors. Cell 81: 769-780, 1995 https://doi.org/10.1016/0092-8674(95)90538-3
  21. Paik JE, Kim N, Yea AA, Jang WH, Chung JY, Lee SK, Park YH, Han J, Seog DH. Kinesin superfamily KIF5 proteins bind to (III spectrin. Korean J Physiol Pharmacol 8: 167-172, 2004
  22. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. 3rd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1989
  23. Seog DH, Lee DH, Lee SK. Molecular Motor Proteins of the Kinesin superfamily proteins (KIFs): structure, cargo and disease. J Korean Medical Science 19: 1-7, 2004 https://doi.org/10.3346/jkms.2004.19.1.1
  24. Setou M, Nakagawa T, Seog DH, Hirokawa N. Kinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport. Science 288: 1796-1802, 2000 https://doi.org/10.1126/science.288.5472.1796
  25. Setou M, Seog DH, Y. Tanaka Y, Kanai Y, Takei Y, Kawagishi M, Hirokawa N. Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites. Nature 417: 83-87, 2002 https://doi.org/10.1038/nature743
  26. Su Q, Cai Q, Gerwin C, Smith CL, Sheng ZH. Syntabulin is a microtubule-associated protein implicated in syntaxin transport in neurons. Nat Cell Biol 6: 941-953. 2004 https://doi.org/10.1038/ncb1169
  27. Takeda S, Yamazaki H, Seog DH, Kanai Y, Terada S, Hirokawa N. Kinesin superfamily protein 3 (KIF3) motor transports fodrin-associating vesicles important for neurite building. J Cell Biol 148: 1255-1265, 2000 https://doi.org/10.1083/jcb.148.6.1255
  28. van Kerkhof P, Lee J, McCormick L, Tetrault E, Lu W, Schoenfish M, Oorschot V, Strous GJ, Klumperman J, Bu G. Sorting nexin 17 facilitates LRP recycling in the early endosome. EMBO J 24: 2851-2861, 2005 https://doi.org/10.1038/sj.emboj.7600756
  29. Wang Y, Zhou Y, Szabo K, Haft CR, Trejo J. Down-regulation of protease-activated receptor-1 is regulated by sorting nexin 1. Mol Biol Cell 13: 1965-1976, 2002 https://doi.org/10.1091/mbc.E01-11-0131
  30. Williams R, Schluter T, Roberts MS, Knauth P, Bohnensack R, Cutler DF. Sorting nexin 17 accelerates internalization yet retards degradation of P-selectin. Mol Biol Cell 15: 3095-3105, 2004 https://doi.org/10.1091/mbc.E04-02-0143
  31. Worby CA, Dixon JE. Sorting out the cellular functions of sorting nexins. Nat Rev Mol Cell Biol 3: 919-931, 2002 https://doi.org/10.1038/nrm974
  32. Yonekawa Y, Harada A, Okada Y, Funakoshi T, Kanai Y, Takei Y, Terada S, Noda T, Hirokawa N. Defect in synaptic vesicle precursor transport and neuronal cell death in KIF1A motor protein-deficient mice. J Cell Biol 141: 431-441, 1998 https://doi.org/10.1083/jcb.141.2.431
  33. Zhao C, Takita J, Tanaka Y, Setou M, Nakagawa T, Takeda S, Yang HW, Terada S, Nakata T, Takei Y, Saito M, Tsuji S, Hayashi Y, Hirokawa N. Charcot-Marie-Tooth disease type 2A caused by mutation in a microtubule motor KIF1Bbeta. Cell 105: 587-597, 2001 https://doi.org/10.1016/S0092-8674(01)00363-4