Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Determination of Methoxyfenozide Residues in Water and Soil by Liquid Chromatography: Evaluation of its Environmental Fate Under Laboratory Conditions

  • Choi, Jeong-Heui (Natural Product Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Mamun, M.I.R. (Natural Product Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Shin, Eun-Ho (Natural Product Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Hee-Kwon (Vegetables Crops Experiment Station, Jeonnam Agricultural Research & Extention Service) ;
  • El-Aty, A.M. Abd (Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University) ;
  • Shim, Jae-Han (Natural Product Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University)
  • Published : 2008.09.01
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Abstract

Pesticide residues play several key roles as environmental and food pollutants and it is crucial to develop a method for the rapid determination of pesticide residues in environments. In this study, a simple, effective, and sensitive method has been developed for the quantitative analysis of methoxyfenozide in water and soil when kept under laboratory conditions. The content of methoxyfenozide in water and soil was analyzed by first purifying the compound through liquid-liquid extraction and partitioning followed by florisil gel filtration. Upon the completion of the purification step the residual levels were monitored through high performance liquid chromatography(HPLC) using a UV absorbance detector. The average recoveries of methoxyfenozide from three replicates spiked at two different concentrations and were ranged from 83.5% to 110.3% and from 98.1% to 102.8% in water and soil, respectively. The limits of detection(LODs) and limits of quantitation(LOQs) were 0.004 vs. 0.012 ppm and 0.008 vs. 0.024 ppm, respectively. The method was successfully applied to evaluate the behavioral fate of a 21% wettable powder(WP) methoxyfenozide throughout the course of 14 days. A first-order model was found to accurately fit the dissipation of methoxyfenozide in water with and a $DT_{50}$ value of 3.03 days was calculated from the fit. This result indicates that methoxyfenozide dissipates rapidly and does not accumulate in water.

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References

  1. Adamczyk, J.J. Jr., Leonard, B.R. and Graves, J.B. (1999). Toxicity of selected insecticides to fall armyworms (Lepidoptera: Noctuidae) in laboratory bioassay studies. Fla. Entomol., 82, 230-236 https://doi.org/10.2307/3496574
  2. Adel, M.M. and Sehnal, F. (2000). Azadirachtin potentiates the action of ecdysteroid agonist RH-2485 in Spodoptera littoralis. J. Insect Physiol., 46, 267-274 https://doi.org/10.1016/S0022-1910(99)00179-1
  3. Allison, L.E. (1965). Organic Carbon. In: Methods of soil analysis, Part II, Ed. C. A. Black, ASA, Madison, WI, pp. 1367-1376
  4. Carlson, G.R., Dhadialla, T.S., Hunter, R., Jasson, R.K., Jany, C.S., Lidert, Z. and Slawecki, R.A. (2001). The chemical and biological properties of methoxyfenozide, a new insecticidal ecdysteroid agonist. Pest Manag. Sci., 57, 115-119 https://doi.org/10.1002/1526-4998(200102)57:2<115::AID-PS245>3.0.CO;2-A
  5. Choi, B.K., Hercules, D.M. and Gusev, A.I. (2001). LC-MS/ MS signal suppression effects in the analysis of pesticides in complex environmental matrices. Fresenius J. Anal. Chem., 369, 370-377 https://doi.org/10.1007/s002160000661
  6. Daughton, C.G. and Ternes, T.A. (1999). Pharmaceuticals and personal care products in the environment: agents of subtle change? Envir. Health Perspect., 107, 907-938 https://doi.org/10.2307/3434573
  7. European Union Guidelines, Document SANCO/10476/2003, Quality control procedures for pesticide residues analysis (2004)
  8. Halling-Sorensen, B., Nors Nielsen, S., Lanzky, P.F., Ingerslev, F., Holten Lutzhoft, H.C. and Jorgensen, S.E. (1998). Occurrence, fate and effects of pharmaceutical substances in the environment. Chemosphere, 36, 357-359 https://doi.org/10.1016/S0045-6535(97)00354-8
  9. Hall, G.L., Engebretson, J., Hengel, M.J. and Shibamoto, T. (2004). Analysis of methoxyfenozide residues in fruits, vegetables, and mint by liquid chromatography-tandem mass spectrometry (LC-MS/MS). J. Agric. Food Chem., 52, 672-676 https://doi.org/10.1021/jf030591n
  10. Hiemstra, M. and Kok, A. (2007). Comprehensive multi-residue method for the target analysis of pesticides in crops using liquid chromatography-tandem mass spectrometry. J. Chromatogr. A, 1154, 3-25 https://doi.org/10.1016/j.chroma.2007.03.123
  11. Hu, D. and Coats, J.R. (2007). Aerobic degradation and photolysis of tylosin in water and soil. Environ. Toxicol. Chem., 26, 884-889 https://doi.org/10.1897/06-197R.1
  12. Korea Food and Drug Administration. 'MRLs for Pesticides in Foods', Seoul: Republic of Korea (2005)
  13. Le, D., Thirugnanam, M., Lidert, Z., Carlson, G.R. and Bryan, J.B. (1996). RH-2485: a new selective insecticide for caterpillar control. Proc. Brighton Crop Protect. Conf.-Pests Dis., 2, 481
  14. Oberlander, H., Silhacek, D.L. and Leach, C.E. (1998). Interactions of ecdysteroid and juvenoid agonists in Plodia interpunctella (Hubner). Arch. Insect Biochem. Physiol., 38, 91-99 https://doi.org/10.1002/(SICI)1520-6327(1998)38:2<91::AID-ARCH5>3.0.CO;2-Y
  15. Olsza, R.W. and Pluciennik, Z. (1998). Preliminary investigations on effectiveness of two modern insecticides in controlling codling moth, plum fruit moth, and leaf rollers. Brighton Crop Prot. Conf.-Pests Dis., 57-62
  16. RDA (Rural Development Administration). Methods of soil chemical analysis. National Institute of Agricultural Science and Technology, RDA, Suwon, Korea (1988)
  17. Relyea, R. and Hoverman, J. (2006). Assessing the ecology in ecotoxicology: a review and synthesis in freshwater systems. Ecol. Lett., 9, 1157-1171 https://doi.org/10.1111/j.1461-0248.2006.00966.x
  18. Smagghe, G., Wesemael, W., Carton, B. and Tirry, L. (1998). Tebufenozide and methoxyfenozide against the beet armyworm, Spodoptera exigua. Brighton Conf.-Pests Dis., 311-312
  19. Smagghe, G., Nakagawa, Y., Carton, B., Mourad, A.K., Fujita, T. and Tirry, L. (1999). Comparative ecdysteroid action of ring-substituted dibenzoylhydrazines in Spodoptera exigua. Arch. Insect Biochem. Physiol., 41, 42-53 https://doi.org/10.1002/(SICI)1520-6327(1999)41:1<42::AID-ARCH7>3.0.CO;2-H
  20. Smagghe, G., Carton, B., Heirman, A. and Tirry, L. (2000). Toxicity of four dibenzoylhydrazine correlates with evagination- induction in the cotton leafworm. Pest. Biochem. Physiol., 68, 49-58 https://doi.org/10.1006/pest.2000.2497
  21. Smagghe, G., Carton, B., Decombel, L. and Tirry, L. (2001). Significance of absorption, oxidation, and binding to toxicity of four ecdysone agonists in multi-resistant cotton leafworm. Arch. Insect Biochem. Physiol., 46, 127-139 https://doi.org/10.1002/arch.1023
  22. Trisyono, A., Goodman, C.L., Grasela, J.J., Mcintosh, A.H. and Chippendale, G.M. (2000). Establishment and characterization of an Ostrinia nubilalis cell line, and its response to ecdysone agonists. In vitro Cell. Dev. Biol. Anim., 36, 400-404 https://doi.org/10.1290/1071-2690(2000)036<0400:EACOAO>2.0.CO;2
  23. Wang, J. and Wotherspoon, D. (2007). Determination of pesticides in apples by liquid chromatography with electrospray ionization tandem mass spectrometry and estimation of measurement uncertainty. J. AOAC Int., 90, 550-567
  24. WHO Report 2003, Food and Agriculture Organization of the United Nations, Rome, 2004. http://www.fao.org/docrep/ 006/Y5221E/y5221e00.HTM
  25. Wing, K.D. (1988). RH-5849, a nonsteroidal ecdysone agonist: effects on a Drosophila cell line. Science, 241, 467-468 https://doi.org/10.1126/science.3393913
  26. Wing, K.D., Slawecki, R.A. and Carlson, G.R. (1988). RH- 5849, a nonsteroidal ecdysone agonist: effects on larval Lepidoptera. Science, 241, 470-472 https://doi.org/10.1126/science.241.4864.470