베타카로틴 함유 제제의 약효 연구 - 뷰티카로틴 제제에 대하여 -

Study on the Pharmacological Effects of Preparations Containing Beta-carotene - On $Beautycarotene^{TM}$ Preparations -

  • 발행 : 2009.10.25

초록

The purpose of this research was to investigate the pharmacological effects of preparations containing beta-carotene, $Beautycarotene^{TM}$. $Beautycarotene^{TM}$ increased the collagen synthesis in CCD-986sk cells, DPPH radical scavenging activity and tyrosinase inhibitory activity in vitro system. In addition, it enhanced the viability of murine thymocytes and the population of splenic $CD4^+$ cells. Also, it increased the phagocytic activity of murine peritoneal macrophages. These results indicate that $Beautycarotene^{TM}$ can have a protective effect of skin via the diverse action, such as the stimulatory action of collagen synthesis, antioxidative action, tyrosinase inhibitory action and immune regulatory action.

키워드

참고문헌

  1. 이원택 외 역. Hole's human anatomy & physiology 9판. 정담, p 168, 2004
  2. Bauza, E., Oberto, G., Berghi, A., Dal, C.F., Domloge, N. Collagen-like peptide exhibits a remarkable antiwrinkle effect on the skin when topically applied: in vivo study. Int J Tissue React., 26(3-4):105-111, 2004
  3. O'Connor, I. and O'Brien, N. Modulation of UVA light-induced oxidative stress by beta-carotene, lutein and astaxanthin in cultured fibroblasts. J. Dermatol. Sci. 16: 226-230, 1998 https://doi.org/10.1016/S0923-1811(97)00058-3
  4. Chew, B.P., Park, J.S. Carotenoid action on the immune response. J. Nutr., 134(1):257S-261S, 2004 https://doi.org/10.1093/jn/134.1.257S
  5. Grassmann, J. Terpenoids as plant antioxidants. Vitam. Horm., 72: 505-535, 2005 https://doi.org/10.1016/S0083-6729(05)72015-X
  6. Muhling, J., Fuchs, M., Campos, M., Gonter, J., Sablotzki, A., Engel, J., Welters, I.D., Wolff, M., Matejec, R., Dehne, M.G., Menges, T., Krüll, M., Hempelmann, G. Effects of ornithine on neutrophil (PMN) free amino acid and alpha-keto acid profiles and immune functions in vitro. Amino Acids., 27(3-4):313-319, 2004 https://doi.org/10.1007/s00726-004-0126-0
  7. Marin, V.B., Rodriguez-Osiac, L., Schlessinger, L., Villegas, J., Lopez, M., Castillo-Duran, C. Controlled study of enteral arginine supplementation in burned children: impact on immunologic and metabolic status. Nutrition. 22(7-8):705-712, 2006 https://doi.org/10.1016/j.nut.2006.03.009
  8. Garner, S.T.Jr., Israel, B.J., Achmed, H., Capomacchia, A.C., Abney, T., Azadi, P. Transdermal permeability of N-acetyl-D-glucosamine. Pharm. Dev. Technol., 12(2):169-174, 2007 https://doi.org/10.1080/10837450701212560
  9. Han, D., Kim, H.Y., Lee, H.J., Shim, I., Hahm, D.H. Wound healing activity of gamma-aminobutyric Acid (GABA) in rats. J. Microbiol. Biotechnol., 17(10):1661-1669, 2007
  10. Lee, E.H., Faulhaber, D., Hanson, K.M., Ding, W., Peters, S., Kodali, S. and Granstein, R.D. Dietry lutein reduces ultraviolet radiation-induced inflammation and immuno- suppression. J. Invest. Dermatol., 122: 510-517, 2004 https://doi.org/10.1046/j.0022-202X.2004.22227.x
  11. Philips, N., Keller, T., Hendrix, C., Hamilton, S., Arena, R., Tuason, M., Gonzalez, S. Regulation of the extracellular matrix remodeling by lutein in dermal fibroblasts, melanoma cells, and ultraviolet radiation exposed fibroblasts. Arch. Dermatol. Res., 299(8):373-379, 2007 https://doi.org/10.1007/s00403-007-0779-0
  12. Oikarinen, A., Autio, P., Kiistala, U., Risteli, L., Risteli, J. A new method to measure type I and III collagen synthesis in human skin in vivo: demonstration of decreased collagen systhesis after topcal glucocorticoid treatment. J. Invest. Dermatol., 98: 220-225, 1992 https://doi.org/10.1111/1523-1747.ep12555884
  13. Blois, M.S. Antioxidant determinations by the use of a stable free radicals. J. Agric. Food. Chem., 25: 103-107, 1977 https://doi.org/10.1021/jf60209a051
  14. Wattasinghe, M., Shahidi, F. Scavenging of reactiveoxigen species and DPPH free radicals by extracts of borage and evenig primrose meals. Food chem., 70: 17-26, 2000 https://doi.org/10.1016/S0308-8146(99)00269-1
  15. Yagi, A., Kanbara, T. and Morinobu, N. The effect of tyrosinase inhibition for Aloe. Planta Med. 3981: 517-519, 1986
  16. Wysocki, L.J. and Sato, V.L. Planning for lymphocytes: A method for cell selection. Proc. Natl. Acad. Sci. USA, 75: 2844, 1978 https://doi.org/10.1073/pnas.75.6.2844
  17. Mizel, S.B., Openheim, J.J. and Rosensteich, D.L. Characterization of lymphocyte-activating factor(LAF) produced by the macrophage cell line P388D1. J. Immunol. 120: 1497, 1979
  18. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J. Immunol. methods., 65: 55, 1983 https://doi.org/10.1016/0022-1759(83)90303-4
  19. Kotnic, V. and Fleischmann, W.R.Jr. A simple and rapid method to determine hematopoietic growth factor activity. J. Immunol. methods., 129: 23, 1990 https://doi.org/10.1016/0022-1759(90)90416-S
  20. Suda, T. and Nagata, S. Purification and characterization of the Fas-ligand that induces apoptosis. J. Exp. Med. 179: 873-879, 1994 https://doi.org/10.1084/jem.179.3.873
  21. Boudard, F., Vallot, N., Cabaner, C. and Bastide, M. Chemiluminenscence and nitrite determinations by the MALU macrophage cell line. J. Immunol. Methods, 174: 259, 1994 https://doi.org/10.1016/0022-1759(94)90030-2
  22. Blair, A.L., Cree, I.A., Beck, J.S. and Hating, M.J.G. Measurement of phagocyte chemiluminenscence in a microtiter plate format. J. Immunol. Methods, 112: 163, 1988 https://doi.org/10.1016/0022-1759(88)90352-3
  23. Korea Food & Drug Administration (KFDA), Guideline for efficacy evaluation of functional cosmetics (II). 11-1470000-000863-01, 2005
  24. Braga, P.C., Dal Sasso, M., Culici, M., Spallino, A., Falchi, M., Bertelli, A., Morelli, R., Lo Scalzo, R. Antioxidant activity of Calendula officinalis extract: inhibitory effects on chemiluminescence of human neutrophil bursts and electron paramagnetic resonance spectroscopy. Pharmacology. 83(6):348-355, 2009 https://doi.org/10.1159/000217583
  25. Yu, M.H., Im, H.G., Lee, H.J., Ji, Y.J., Lee, I.S. Components and their Antioxidantive Activities of Methanol Extracts from Sarcocarp and Seed of Zizyphus jujuba var. inermis Rehder. Food Sci. Technol., 38: 128-134, 2006
  26. Lee, S.J., Park, D.W., Jang, H.G., Kim, C.Y., Park, Y.S., Kim, T.C., Heo, B.G. Total Phenol Content, Electron Donating Ability, and Tyrosinase Inhibition Activity of Pear Cut Branch Extract. Kor. J. Hort. Sci. Technol. 24(3):338-341, 2006
  27. Hwang, J.H., Lee, B.M. Inhibitory Effects of Plant Extracts on Tyrosinase, l-DOPA Oxidation, and Melanin Synthesis. J. Toxicol. Environ. Health. A. 70(5):393-407, 2007 https://doi.org/10.1080/10937400600882871
  28. Korner, A., Pawelek J. Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin. Science, 217: 1163-1165, 1982 https://doi.org/10.1126/science.6810464
  29. An, B.J., Cho, Y.J., Son, J.H., Pack, J.M. Lee, J.Y., Pack, T.S.Antioxidant effects and Tyrosinase Inhibition Activity of Extract of Prunus sargentii Rehder. J. Kor. Soc. Appl, Biol. Chem., 49(2):145-148, 2006
  30. Jeong, J.H., Wee, J.J., Shin, J.Y., Cho, J.H., Jung, D.H. Antioxidative effect of crude saponin fraction prepared from culture product of basidiomycota cultured with fresh ginseng as substrate, Korean J. Food Sci. Technol. 37: 67-72, 2005
  31. Kim, S.H., Kim, I.C. Antioxidative properties and whitening Effects of the Eucommiae cortex, Salviae miltiorrlhizae radix, Aurantii nobilis pericarpium and Cnidii rhizoma, J. East. Asian. Soc. Dietary Life, 18(4):618-623, 2008
  32. Miceli, M.C., Parnes, J.R. The role of CD4 and CD8 in T cell activation and differentiation. Advances in Immunology, 53: 59-63, 1993 https://doi.org/10.1016/S0065-2776(08)60498-8
  33. Breiheim, G., Stendahl, O. and Dahlgren, C. Intra- and extracellular events in luminol-dependent chemilulinescence of polymorphonuclear leukocytes. Infect. Immun., 45: 1, 1984