Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Genotoxicity Studies on Carrageenan: Short-term In Vitro Assays

  • Chung, Young-Shin (Medvill Co., Ltd.) ;
  • Eum, Ki-Hwan (Department of Biology, College of Natural Sciences, University of Incheon) ;
  • Choi, Seon-A (Medvill Co., Ltd.) ;
  • Oh, Se-Wook (Korea Food Research Institute) ;
  • Park, Sue-Nie (Division of Genetic Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Yum, Young-Na (Division of Genetic Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Joo-Hwan (Division of Genetic Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Seo, Young-Rok (Department of Pharmacology, Institute for Basic Medical Science, Kyung Hee University) ;
  • Lee, Michael (Department of Biology, College of Natural Sciences, University of Incheon)
  • Published : 2009.03.01
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Abstract

Carrageenan is a naturally-occurring sulfated polygalactan which has been widely used in the dairy industry and a gelling agent in non-dairy products. In this study, four short-term in vitro genotoxicity assays were investigated to evaluate the potential genotoxic effects of carrageenan. The mutagenicity of carrageenan was evaluated up to a maximum dose of 5 mg/plate in Ames test. There was no increase in the number of revertant colonies compared to its negative control at any dose in all of strains tested. To assess clastogenic effect, the in vitro chromosomal aberration assay was performed using Chinese hamster lung cells. Carrageenan was not considered to be clastogenic in this assay at up to the highest feasible concentration which could be evaluated. The in vitro comet assay and micronucleus test results obtained on L5178Y cells also revealed that carrageenan has no genotoxicity potential, although there was a marginal increase in micronuclei frequencies and DNA damage in the respective micronucleus and comet assays. Taken together, our results indicate that carrageenan was not genotoxic based on four in vitro genotoxicity results.

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References

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