Asian-Australasian Journal of Animal Sciences

  • 제22권4호
  • /
  • Pages.492-499
  • /
  • 2009
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Establishment and Characterization of the Fibroblast Line from Silkie Bantam

  • Li, L.F. (Institute of Animal Science, Chinese Academy of Agricultural Science) ;
  • Guan, W.J. (Institute of Animal Science, Chinese Academy of Agricultural Science) ;
  • Li, H. (Institute of Animal Science, Chinese Academy of Agricultural Science) ;
  • Bai, X.J. (College of Animal Science and Technology, Northeast Agricultural University) ;
  • Ma, Y.H. (Institute of Animal Science, Chinese Academy of Agricultural Science)
  • 투고 : 2008.08.20
  • 심사 : 2009.01.05
  • 발행 : 2009.04.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A Silkie Bantam embryo fibroblast line (named SBF59 line) was successfully established by using direct explant culture and cryopreservation techniques. Cell morphology, viability, dynamic growth and contamination were tested and the karyotype and levels of isoenzymes of lactic dehydrogenase and malic dehydrogenase were analyzed. Four kinds of fluorescent protein extrogenes, including $pEGFP-N_3$, $pECFP-N_1$, $pEYFP-N_1$ and $pDsRed1-N_1$ were transfected into the cells. The results showed that the cells were healthy and possessed a fibrous structure without a change in morphology. The average viability of the cells was 96% before freezing and 90.5% after thawing. The growth curve appeared as typical "S" shape and the cell growth passed through a detention phase, a logarithmic phase and a platform phase; the estimated population doubling time (PDT) was 38.5 h; assays for the presence of bacteria, fungi, viruses and mycoplasmas were negative; the cell line showed no cross contamination when assessed by isoenzyme analysis; the chromosome number was 2n = 78 on more than 88% of occasions; four kinds of fluorescent protein extro-genes appeared to be expressed effectively with a high transfection efficiency between 18.3% and 42.3%. The cell line met the required quality control standard. It not only preserves the genetic resources of the important Silkie Bantam at the cellular level but also provides valuable materials for genomic, post-genomic, somatic cell cloning research and other applications.

키워드

참고문헌

  1. Chen, Y., J. Zhao, H. Q. Zhang and H. M. Zhou. 2006. Establishment of fibroblast line of adult sheep skin. J. Inner Mongolia Agric. Univ. (Natural Science Edition) 2:13-16
  2. Doyle, A., C. Morris and J. M. Mowles. 1990. Quality control. In: Animal cells, living resources for biotechnology (Ed. A. Doyle, R. Hay and B. E. Kirsop). Cambridge University Press, Cambridge, UK.
  3. Freshney, R. I. 1992. Animal cell culture: a practical approach. Oxford University Press. pp. 119-122
  4. Gu, Y., H. Li, J. Miki, K. H. Kim, B. Furusato, I. A. Sesterhenn, W. S. Chu, D. G. McLeod, S. Srivastava, C. M. Ewing, W. B. Isaacs and J. S. Rhim. 2006. Phenotypic characterization of telomerase-immortalized primary non-malignant and malignant tumor-derived human prostate epithelial cell lines. Exp. Cell Res. 312(6):831-843 https://doi.org/10.1016/j.yexcr.2005.11.029
  5. Guan, W. J., Y. H. Ma, H. Ding, T. Y. Yu, H. Y. Zhang and H. Q. Liang. 2005a. The establishment of fibroblast cell line and its biological characteristic research in Small Tail Han sheep. Chin. J. Anim. Vet. Sci. 5:511-516
  6. Guan, W. J., Y. H. Ma, X. Y. Zhou, G. L. Liu and X. D. Liu. 2005b. The establishment of fibroblast cell line and its biological characteristic research in Taihang Black Goat. Rev. China Agric. Sci. Technol. 5:25-33
  7. Ho, S. K., E. E. Lister and S. P. Touchbum. 1997. An overview of Canadian farm animal genetic resources conservation and its associated biotechnological approaches. Proceedings of International Conference of Animal Biotechnology, Beijing, China. pp. 155-160
  8. Hong, S. B., S. J. Uhm, H. Y. Lee, C. Y. Park, M. K. Gupta, B. H. Chung, K. S. Chung and H. T. Lee. 2005. Developmental ability of bovine embryos nuclear transferred with frozenthawed or cooled donor cells. Asian-Aust. J. Anim. Sci. 18(9):1242-1248
  9. Kawarai, S., K. Hashizaki, S. Kitao, S. Nagano, H. Madarame, S. Neo, T. Ishikawa, M. Furuichi, M. Hisasue, R. Tsuchiya, H. Tsujimoto and T. Yamada. 2006. Establishment and characterization of primary canine hepatocellular carcinoma cell lines producing alpha-fetoprotein. Vet. Immunol. Immunopathol. 113(1-2):30-36 https://doi.org/10.1016/j.vetimm.2006.03.006
  10. Kim, D. Y. and S. L. McElroy. 2008. Optimization of procedure for efficient gene transfer into porcine somatic cells with lipofection. Asian-Aust. J. Anim. Sci. 21(5):648-656
  11. Lee, Y. S., S. A. Ock, S. K. Cho, B. G. Jeon, T. Y. Kang, S. Balasubramanian, S. Y. Choe and G. J. Rho. 2007. Effect of donor cell types and passages on preimplantation development and apoptosis in porcine cloned embryos. Asian-Aust. J. Anim. Sci. 20(5):711-717
  12. Liu, C. Q., Y. Guo, W. J. Guan, Y. H. Ma, H. H. Zhang and X. Tang. 2008. Establishment and biological characteristic of Luxi Cattle fibroblast bank. Tissue Cell. 40(6):417-424 https://doi.org/10.1016/j.tice.2008.04.005
  13. Lu, H. N., C. S. Liu and Z. G. Wang. 2007. The establishment of fibroblast cell line and its biological characteristic research in guide black fur sheep. China Herbivores. 2:3-6
  14. Ma, Y. H., X. M. Zhou, W. J. Guan and D. M. Zhao. 2004. Establishment and identification of Debao pony ear marginal tissue fibroblast cell line. Asian-Aust. J. Anim. Sci. 17(10):1338-1343
  15. MacLeod, R. A., W. G. Dirks, Y. Matsuo, M. Kaufmann, H. Milch and H. G. Drexler. 1999. Widespread intraspecies crosscontamination of human tumor cell lines arising at source. Int. J. Cancer. 83(4):555-563 https://doi.org/10.1002/(SICI)1097-0215(19991112)83:4<555::AID-IJC19>3.0.CO;2-2
  16. Nims, R. W., A. P. Shoemaker, M. A. Bauternschub, L. J. Rec and J. W. Harbell. 1998. Sensitivity of isoenzyme analysis for the detection of interspecies cell line cross-contamination. In vitro Cell Dev. Biol. Anim. 34:35-39 https://doi.org/10.1007/s11626-998-0050-9
  17. Oishi, T. 1997. Conservation and evaluation of animal genetic resources. Farming Jpn. 6:18-25
  18. Pan, Q. Z., L. Tian, S. G. Xu, H. B. Han, J. Li, H. Li, S. F. Sun, Z. X. Lian, N. Yang, N. Li and J. H. Tan. 2006. A method established for goat fibroblast in vitro. J. China Agric. Univ. 1:29-34
  19. Park, H. S., S. Y. Jung, T. S. Kim, J. K. Park, T. S. Moon, S. P. Hong, J. I. Jin, J. S. Lee, J. H. Lee, S. H. Sohn, C. Y. Lee and Y. S. Moon. 2007. Production of cloned Korean Native Goat (Capra hircus) by somatic cell nuclear transfer. Asian-Aust. J. Anim. Sci. 20(4):487-495
  20. Park, M. C., J. Y. Kim, S. B. Kim, Y. S. Park, H. D. Park, J. H. Lee, D. S. Oh and J. M. Kim. 2009. The effect of cryopreservation on the mouse embryos at various-pronuclear Stages. Asian-Aust. J. Anim. Sci. 22(2):174-180
  21. Qi, Y., Y. Tu, D. Yang, Q. Chen, J. Xiao, Y. Chen, J. Fu, X. Xiao and Z. Zhou. 2007. Cyclin A but not cyclin D1 is essential in cmyc-modulated cell cycle progression. J. Cell Physiol. 210:63-71 https://doi.org/10.1002/jcp.20816
  22. Shi, L. M. 1989. Freezing zoo: 1. Establishment and application of wild animal cell banks. Biol. Bull. 6:1-3
  23. Simon, D. L. 1999. Better decisions in conservation of farm animal genetic resources by use of international sources of information. Information in Decision Making in Agricultural Research and Practice/IAALD QB XLIV, 1/2., Freising Press, Germany
  24. Suemori, H., K. Yasuchika, K. Hasegawa, T. Fujioka, N. Tsuneyoshi and N. Nakatsuji. 2006. Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage. Biochem. Biophys. Res. Commun. 345(3):926-932 https://doi.org/10.1016/j.bbrc.2006.04.135
  25. Tsuchiya, R., F. Yoshiki, Y. Kudo and M. Morita. 2002. Cell typeselective expression of green fluorescent protein and the calcium indicating protein, yellow cameleon, in rat cortical primary cultures. Brain Res. 956(2):221-229 https://doi.org/10.1016/S0006-8993(02)03518-7
  26. Wang, X. G., G. H. Chen, X. Y. Zhang, Z. Y. Fu, Q. Xu and B. C. Li. 2003. Study on Luyuan Chicken's karyotype and the correlation with the productive performance. China Poult. 6:10-12
  27. Werners, A. H., S. Bull, J. Fink-Gremmels and C. E. Bryant. 2004. Generation and characterisation of anequine macrophage cell line (e-CAS cells) derived from equine bone marrow cells. Vet. Immunol. Immunopathol. 97(1-2):65-76 https://doi.org/10.1016/j.vetimm.2003.08.012
  28. Wu, C. X. 1999. Theory and technique of animal genetic resources conservation. Idioplasmic basis for persist development of animal agriculture at 21 century. J. Yun Nan Univ. 21:7-10
  29. Wu, H. M., W. J. Guan, H. Li and Y. H. Ma. 2008. Establishment and characteristics of White Ear Lobe Chicken embryo fibroblast line and expression of six fluorescent proteins in the cells. Cell Biol. Int. 32(12):1478-1485 https://doi.org/10.1016/j.cellbi.2008.08.006
  30. Xu, Q., G. H. Chen, X. Y. Zhang, B. C. Li, X. S. Wu, L. Liu and B. H. Ji. 2004. Comparison on karyotype of three chinese native chickens and their cluster analysis. China J. Anim. Vet. Sci. 4:362-366
  31. Xue, Q. S. 2001. The principle and technique of in vitro culture. Science Press, Beijing, China. pp. 432-444
  32. Yun, J. I., B. S. Koo, S. W. Yun and C. K. Lee. 2008. In vitro development of interspecies somatic cell nuclear transfer embryos derived from murine embryonic fibroblasts and bovine oocytes. Asian-Aust. J. Anim. Sci. 21(11):1665-1672
  33. Zeng, K. W. and F. Y. Chen. 1997. LDH isoenzyme of vertebrate animals. Haerbin Industrial Press, Haerbin, China
  34. Zhang, H. R., H. C. Yu, Z. Q. Wei and R. Z. Chen. 2000. The relationship of double lambs and serum lactate dehydrogenase isoenzyme of Tan Sheep. Gansu Anim. Vet. Sci. 4:13-14
  35. Zhang, L. J., H. F. Liu and X. W. Li. 2008. The establishment of fibroblast cell line and its biological characteristic research in Taihu Pig. J. Sichuan Agric. Univ. 1:85-88
  36. Zhou, X. M., Y. H. Ma, W. J. Guan, J. Wen and H. Li. 2005. Establishment and characteristics of a Beijing fatty chicken embryo fibroblast cell line. China J. Anim. Vet. Sci. 3:209-215 https://doi.org/10.1016/j.yexcr.2005.11.029