Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Some Factors Affecting Freezing of Boar Semen in 5 ml Maxi-straws

  • Dai, J.J. (Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences) ;
  • Wu, C.F. (Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences) ;
  • Zhang, Defu (Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences) ;
  • Yin, F.Z. (Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences) ;
  • Zhang, T.Y. (Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences) ;
  • Liu, D. (Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences) ;
  • Wu, H.L. (Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences) ;
  • Li, L.L. (Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences) ;
  • Yang, S.T. (Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences) ;
  • Wang, L. (Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences)
  • Received : 2008.09.24
  • Accepted : 2008.12.26
  • Published : 2009.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

A series of experiments were conducted to determine the suitable freezing and thawing temperatures for the freezing of boar semen in 5 ml maxi-straws. The ultrastructure, in vitro fertilization (IVF) and artificial insemination (AI) of frozen-thawed semen were also be evaluated. The 5 cm freezing height gave the best results not only in post-thaw motility rate (54.00%), but also in normal acrosome morphology rate (NAR) (80.23%). There was no significant difference in the post-thaw motility between different thawing temperatures and corresponding thawing times (p>0.05); the group of $52^{\circ}C$ and 25 s gave the highest motility rate (45.00%). As a whole, not only from the motility but also the NAR, thawing at $42^{\circ}C$ was better than the other two treatments. In the freezing packages, 5 ml maxi-straw gave a little lower mobility (40%), viability rate (49.58%), plasma membrane integrity rate (53.91%) and NAR (52.65%) than the 0.25 ml straw, but there was no significant difference between the two straw volumes (p>0.05). The IVF capacity of frozen-thawed semen in this experiment was similar to fresh semen. From ultrastructure observation, the main damage to boar spermatozoa after freezing was seen in the acrosome, such as swelling and formation of vesicles. After AI in recipient Shanghai White sows, frozen-thawed semen from 5 ml maxi-straws and pellets produced 72.2% and 80% conception rate and 7.8 and 8 litter sizes, respectively, and there was no significant difference between the 5 ml maxi-straw and the pellet (p>0.05).

Keywords

References

  1. Abeydeera, L. R and B. N. Day. 1997. Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified Tris-buffered medium with frozen-thawed ejaculated spermatozoa. Biol. Reprod. 57:729-734 https://doi.org/10.1095/biolreprod57.4.729
  2. Almlid, T., S. E. Stavne and L. A. Johnson. 1987. Fertility evaluation of the straw freezing technique for boar semen under practical I.A. conditions. Zuchthygiene (Beri). 22:193-202 https://doi.org/10.1111/j.1439-0531.1987.tb00537.x
  3. Almlid, T. and P. O. Hofmo. 1996. A brief review of frozen semen application under Norwegian AI service conditions. Reprod. Dom. Anim. 31:169-173 https://doi.org/10.1111/j.1439-0531.1995.tb00021.x
  4. Amann, R. P. 2005. Weaknesses in reports of "fertility" for horses and other species. Theriogenol. 63:698-715 https://doi.org/10.1016/j.theriogenology.2004.04.010
  5. Berger, T., K. O. Turner, S. Meizel and J. L. Hedrick. 1985. Zona pellucida induced acrosome reaction in boar sperm. Biol. Reprod. 40:525-530 https://doi.org/10.1095/biolreprod40.3.525
  6. Bwanga, C. O., M. M. de Braganca, S. Einarsson and H. Rodriguez-Martinez. 1990. Cryopreservation of boar semen in Mini- and Maxi-straws. J. Vet. Med. A, 37:651-658 https://doi.org/10.1111/j.1439-0442.1990.tb00958.x
  7. Bwanga, C. O. 1991. Cryopreservation of boar semen. Acta. Vet. Scand. 32:431-453
  8. Cabrita, E., V. Robles, R. Alvarez and M. P. Herráez. 2001. Cryopreservation of rainbow trout sperm in large volume straws: application to large scale fertilization. Aquaculture. 201(3-4):301-314 https://doi.org/10.1016/S0044-8486(01)00636-6
  9. Cheon, Y. M., H. K. Kim, C. B. Yang, Y. J. Yi and C. S. Park. 2002. Effect of season influencing semen characteristics, frozenthawed sperm viability and testosterone concentration in Duroc boars. Asian-Aust. J. Anim. Sci. 15(4):500-503
  10. Corcuera, B. D. 1996. Efecto de la presi´on osm´otica de los diluyentes de congelaci´on sobre la viabilidad del espermatozoide de verraco. Thesis UCM, School of Biological Sciences, Madrid
  11. Cordova-Izquierdo, A., J. H. Oliva, B. Lleo, C. Garcia-Artiga, B. D. Corcuera and J. F. Perez-Gutierrez. 2006. Effect of different thawing temperatures on the viability, in vitro fertilizing capacity and chromatin condensation of frozen boar semen packaged in 5 ml straws. Anim. Reprod. Sci. 92:145-154 https://doi.org/10.1016/j.anireprosci.2005.05.011
  12. Cordova, A., J. F. Perez, B. Lleo, C. Garcia Artiga and S. Martin Rillo. 2001. In vitro fertilizing capacity of deep frozen boarsemen packaged in 0.5 and 5 ml straws. Reprod. Domest. Anim. 36:199-202
  13. Courtens, J. L. and M. Paquignon. 1985. Ultrastructure of fresh, frozen and frozen-thawed spermatozoa of the boar. In: Deep freezing of boar semen (Ed. L. A. Johnson and K. Larsson). Swedish University of Agricultural Sciences. Uppsala, pp. 61-87
  14. Eriksson, B. M. and H. Rodriguez-Martinez. 2000. Effect of freezing and thawing rates on the post-thaw viability of boar spermatozoa. Anim. Reprod. Sci. 63:205-220 https://doi.org/10.1016/S0378-4320(00)00171-8
  15. Fiser, P. S. 1990. Combined effect of glycerol concentration and cooling velocity on mobility and acrosomal integrity of boar spermatozoa frozen in 0.5 ml straws. Mol. Reprod. Dev. 25:123-129 https://doi.org/10.1002/mrd.1080250204
  16. Fiser, P. S., R. W. Fairfull, C. Hansen, P. L. Panich, J. N. B. Shrestha and L. Underhill. 1993. The effect of warming velocity on motility and acrosome integrity of boar sperm as influenced by the rate of freezing and glycerol level. Mol. Reprod. Dev. 34:190-195 https://doi.org/10.1002/mrd.1080340211
  17. Gil, M. A., L. R. Abeydeera, B. N. Day, J. M. Vazquez, J. Roca and E. Martinez. 2003. Effects of the volume of medium and number of oocytes during in vitro fertilizagtion on embryo development in pigs. Theriogenol. 60:767-776 https://doi.org/10.1016/S0093-691X(03)00051-7
  18. Hammitt, D. G., P. A. Martin and T. Callanan. 1989. Correlations between heterospermic fertility and assays of porcine seminal quality before and after cryopreservation. Theriogenol. 32:385-399 https://doi.org/10.1016/0093-691X(89)90005-8
  19. Jiang, Z. L., Q. W. Li, W. Y. Li, J. H. Hu, H. W. Zhao and S. S. Zhang. 2007. Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay. Anim. Reprod. Sci. 99:401-407 https://doi.org/10.1016/j.anireprosci.2006.08.022
  20. Johnson, L. A., J. G. Aalbers, C. M. T. Willems and W. Sybema. 1981. Use of boar spermatozoa for artificial insemination. I. Fertilizing capacity of fresh and frozen spermatozoa in sows in 36 farms. J Anim Sci. 52:1130-1136 https://doi.org/10.1016/0093-691X(82)90049-8
  21. Mazur, P. 1984. Freezing of live cells: mechanisms and implications. Am. J. Physiol. 247:125-142
  22. Nagai, T., K. Niwa and A. Iritani. 1984. Effect of sperm concentration during preincubation in a defined medium on fertilization in vitro of pig follicular oocytes. J. Reprod. Fertil. 70:271-275 https://doi.org/10.1016/0093-691X(94)00044-U
  23. Nagai, T., T. Takahashi, H. Masuda, Y. Shioya, M. Kuwayama, M. Fukushima, S. Iwasaki and A. Hamada. 1988. In vitro fertilization of frozen boar spermatozoa. J. Reprod. Fertil. 84:585-591 https://doi.org/10.1530/jrf.0.0840585
  24. Pursel, V. G and L. A. Johnson. 1975. Freezing of boar spermatozoa: fertilizing capacity with concentrated semen and a new thawing procedure. J. Anim. Sci. 40:99-102
  25. Pursel, V. G and C. S. Park. 1985. Freezing and thawing procedures for boar spermatozoa (Ed. L. A. Johnson and K. Larsson). Deep freezing of boar semen. Uppsala, Sweden:147-166
  26. Pursel, V. G. and C. S. Park. 1987. Duration of thawing on post thaw acrosome morphology and motility of boar spermatozoa frozen in 5 ml maxi-straws. Theriogenol. 28:683-690 https://doi.org/10.1016/0093-691X(87)90285-8
  27. Royere, D., S. Hamamah, J. C. Nicolle and J. Lansac. 1991. Chromatin alterations induced by freeze-thawing influence the fertilizing ability of human sperm. Int. J. Androl. 14:328-332 https://doi.org/10.1111/j.1365-2605.1991.tb01100.x
  28. Salamon, S., I. Wilmut and C. Polge. 1973. Deep freezing of boar semen. I. Effects of diluent composition, protective agents, and method of thawing on survival of spermatozoa. Aust. J. Biol. Sci. 26:219-230
  29. Sell$\acute{e}$s, J., J. Gadea, R. Romar, C. Matás and S. Ruiz. 2003. Analysis of in vitro fertilizing capacity to evaluate the freezing procedures of boar semen and to predict the subsequent fertility. Reprod. Dom. Anim. 38:66-72 https://doi.org/10.1046/j.1439-0531.2003.00406.x
  30. Soderquist, L., N. Madrid-Bury and H. Rodriguez-Martinez. 1997. Assessment of ram sperm membrane integrity following different thawing procedures. Theriogenol. 48:115-1125
  31. Thomas, C. A., D. L. Garner, M. J. DeJarnette and C. E. Marshall. 1998. Effect of cryopreservation on bovine sperm organelle function and viability as determined by flow cytometry. Biol. Reprod. 58:786-793 https://doi.org/10.1095/biolreprod58.3.786
  32. Wang, W. H., K. Niwa and K. Okuda. 1991. In vitro penetration of pig oocytes matured in culture by frozen-thawed ejaculated spermatozoa. J. Reprod. Fertil. 93:491-496 https://doi.org/10.1016/S0093-691X(97)00034-4
  33. Watson, P. F. 1995. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod. Fertil. Dev. 7:871-889 https://doi.org/10.1071/RD9950871
  34. Weitze, K. F., D. Rath and G. Baron. 1987. Deep freezing of boar semen in plastic straws. Dstch.Tierartzl. Wschr. 94:485-488 https://doi.org/10.1016/S0093-691X(02)00701-X
  35. Westendorf, P., L. Richter and H. Treu. 1975. Zur Tiefgefrierung von ebersperma labor-und besamungsergebnisse mit dem hulsenberger pailletten-verfahren. Dstch. Tierartzl. Wschrr. 82:261-267
  36. Woelders, H. and D. Besten. 1993. Cryopreservation of boar semen with small between- boar variation of post- thaw sperm survival. Cryobiol. 30:645 https://doi.org/10.1016/S0093-691X(02)00947-0
  37. Woelders, H. and A. P. Malva. 1998. How important is the cooling rate in cryopreservation of (bull) semen, and what is its relation to thawing rate and glycerol concentration? Reprod. Dom. Anim. 33:299-305 https://doi.org/10.1111/j.1439-0531.1998.tb01361.x
  38. Wu, C. H., R. Rui, J. J. Dai, C. Y. Zhang, S. Q. Ju, B. Xie, X. Lu and X. F. Zheng. 2006. Dffects of cryopreservation on the developmental compentence, ultrastructure and cytoskeletal structure of porcine oocytes. Mol. Reprod. Dev. 73:1454-1462 https://doi.org/10.1002/mrd.20579
  39. Yi, Y. J., H. J. Ko, S. H. Lee, C. B. Yang, D. S. Son, H. K. Kim and C. S. Park. 2004. In vitro fertilization and development of pig oocytes inseminated with boar sperm by different sperm washing media after thawing of the frozen straws. Asian-Aust. J. Anim. Sci. 17(2):164-167
  40. Zhou, J. B., K. Z. Yue, M. J. Luo, Z. L. Chang, H. Liang, Z. Y. Wang and J. H. Tan. 2004. Effect of extenders and temperatures on sperm viability and fertilizing capacity of Harbin white boar smen during long-term liqudi storage. Asian-Aust. J. Anim. Sci. 17(11):1501-1508

Cited by

  1. Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing vol.21, pp.6, 2009, https://doi.org/10.1631/jzus.b1900520