Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-Inflammatory Effects and Cytoprotective Effects of Smilacis Chinae Radix

토복령의 항염증 및 세포보호 효과에 미치는 영향

  • Lee, Seon-Goo (Department of Oriental Pathology, College of Oriental Medicine, Sangji University)
  • 이선구 (상지대학교 한의과대학 병리학교실)
  • Published : 2009.02.25
Download PDF
⟨ Previous Next ⟩

Abstract

Smilacis Chinae Radix has been used as an anti-inflammatory agent. This study was performed to anti-inflammatory and MAP kinase signaling pathway in vitro. Experimental studies were obtained by measuring the Cytotoxicity, production of NO, PGE2, TNF-$\alpha$ and protein level of catalase, SOD, MAP kinase, The results were summarized as follows: Smilacis Chinae Radix was not cytotoxic effects against Raw264.7 and HEK293 cells. Concentration of $100{\mu}g/m{\ell}$ Smilacis Chinae Radix inhibited the production of NO in the Raw264.7 cell stimulated with LPS. All concentrations of Smilacis Chinae Radix not significantly inhibited the production of PGE2 in the Raw264.7 cell stimulated with LPS. All concentrations of Smilacis Chinae Radix did not inhibit the production of TNF-$\alpha$ in the Raw264.7 cell stimulated with LPS. Smilacis Chinae Radix has not effect of blocking NF-${\kappa}B$ into nucleus in LPS-induced macrophage Raw264.7 cell. Smilacis Chinae Radix has the effect of Cytoprotection through activation of ERK and inhibition of p38 and JNK. Accordingly the results show Smilacis Chinae Radix could induce anti-inflammation and Cytoprotection effects against In vitro, but it needs more research on the precise mechanism of such effects.

본 연구는 토복령의 항염증 및 세포보호 효과에 미치는 영향에 관한 것으로서 주요 내용은 다음과 같다. 본 실험에서는 세포독성, NO의 생성, PGE2, TNF-$\alpha$와 카탈라아제 농도, SOD, MAP kinase 등을 측정하였다. 본 실험의 결과 토복령 추출물은 세포 독성이 없었고 NO의 생성을 억제하며, 항염증과 세포보호 효과가 있었다. 그러나 이러한 효과에 대한 명확한 메커니즘에 대해서는 좀 더 연구가 필요하다는 내용이다.

Keywords

References

  1. 이시진. 본초강목 상책 1294. 인민위생출판사, 1959
  2. 난파항웅. 원색화한약도감 12, 보육사. 1980
  3. Son, K.H., Seo, J.H., Lee, J.M., Kwon, S.J., Chang, S.Y., Lee, K.S. lsolation and quantitative determination of dioscin from Smilacis Chinae Radix. Kor J Pharmacogn 32: 153-156, 2001
  4. Song, J.H., Kwon, H.D., Lee, W.K., Park, I.H. Antimicrobial activity of crude extracts from Smilax China Root. Kor J Biotechnol Bioeng 12: 560-564, 1997
  5. Kim, S.W., Chung, K.C., Son, K.H., Kang, S.S. Steroidal saponins from the Rhizomes of Smilax china. Kor J Pharmacogn 20: 76-82, 1989
  6. 대한병리학회. 병리학. 고문사, pp 71-73, 1994
  7. T. Mosmann. Rapid colorimetric assay for cellular growth and survival application of proliferation and cytotoxicity. J Immunol Methods 65: 55, 1983 https://doi.org/10.1016/0022-1759(83)90303-4
  8. S.M. Han, K.G. Lee, J.H. Yeao, H.Y. Kweon, K.K. Park. Anti-inflammatory effect of the venom from asian honeybees (Apis cerana L.) on inducible nitric oxide synthase and tumor necrosis factor-$\alpha$ in Raw 264.7 cell line. Korean J Apiculture 19: 89, 2004
  9. 전국한의과대학 본초학교수. 본초학. 영림사, pp 211-212, 1991
  10. 차배천, 이은희, 노미애. 토복령의 항산화 활성. 생약학회지 36(3):195-200, 2005
  11. 손건호, 서정학, 이주미, 권순진, 장승엽, 이경순. 토복령으로부터 Dioscin의 분리 및 함량분석. 생약학회지 32(2):153-156, 2001
  12. 박종희, 김정묘, 도원임. 토복령의 생약학적 연구. 생약학회지 33(3):169-172, 2002
  13. 정혜윤, 강성길, 김용철, 박영배, 고형균, 김창환. 토복령약침자극이 $HgCl_2$중독 흰쥐의 손상신에 미치는 영향. 대한침구학회지 15(2):331-348, 1998
  14. 오승규, 박종오, 남궁욱, 강탁림, 김동희. 토복령이 IL-$1{\beta}$와 TNF-$\alpha$로 유발된 Inflammatory Cytokines의 발현 및 생성에 미치는 영향. 대한본초학회지 18(1):49-64, 2003
  15. Zedler, S., Faist, E. The impact of endogenous trighers on trauma-associated inflammation. Current Opinim in Critical Care 12: 595-601, 2006 https://doi.org/10.1097/MCC.0b013e3280106806
  16. Vane, J.R., Mitchell, J.A., Appleton, A., Bishop-Bailey, D., Croxtall, J., Willoughby, D.A. Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation. Proceedings of the National Academy of Science of the United States of America 91: 2046-2050, 1994 https://doi.org/10.1073/pnas.91.6.2046
  17. Moncada, S., Palmer, R.M., Higgs, E.A. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev 43: 109-142, 1991
  18. McCann, S.M., Mastronardi, C., Laurentiis, A. de, Rettori, V. The nitric oxide theory og aging revisited. Annals of the New York Academy of Science 1057: 64-84, 2005 https://doi.org/10.1196/annals.1356.064
  19. 대한병리학회. 간추린병리학. 정문각, pp 63-79, 2002
  20. Murphy, S. Production of nitric oxide by glial cells : regulation and potential roles in the CNS. Glia 29: 1-13, 2000 https://doi.org/10.1002/(SICI)1098-1136(20000101)29:1<1::AID-GLIA1>3.0.CO;2-N
  21. Li, N., Karin, M. Is NF-${\kappa}B$ the sensor of oxidative stress?. FASEB J 13: 1137-1143, 1999 https://doi.org/10.1096/fasebj.13.10.1137
  22. Kyriakis, J.M., Avruch, J. Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation. Physiol Rev 81(2):807-869, 2001 https://doi.org/10.1152/physrev.2001.81.2.807
  23. Zhengui Xia, Martin Dickens, Joel raingeaud, Roger J. Davis, Michael E. Geenberg. Opposing Effects of ERK and JNK-p38 MAP Kinases on Apoptosis. Science 270(5240):1326-1331, 1995 https://doi.org/10.1126/science.270.5240.1326