Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibitory Effect of Extract of Euonymus alatus (Thunb.) Sieb. on the Production of Inflammatory Cytokines

귀전우(鬼箭羽) 추출물의 염증성세포활성물질 억제효과

  • Kim, Dae-Hyeon (Department. of Pharmacy, College of Oriental Pharmacy, Wonkwang University) ;
  • Kim, Byung-Jin (Department. of Pharmacy, College of Oriental Pharmacy, Wonkwang University) ;
  • Park, Kyung-Bae (Department. of Pharmacy, College of Oriental Pharmacy, Wonkwang University) ;
  • Lee, Ju-Sung (Department of Circulatory Internal Medicine, Wonkwang University) ;
  • Sung, Kang-Keyng (Department of Circulatory Internal Medicine, Wonkwang University) ;
  • Park, Sung-Joo (Department of Herbology, College of Oriental Medicine, Wonkwang University)
  • 김대현 (원광대학교 약학대학 한약학과) ;
  • 김병진 (원광대학교 약학대학 한약학과) ;
  • 박경배 (원광대학교 약학대학 한약학과) ;
  • 이주성 (원광대학교 한의과대학 심계내과학교실) ;
  • 성강경 (원광대학교 한의과대학 심계내과학교실) ;
  • 박성주 (원광대학교 한의과대학 본초학교실)
  • Published : 2009.04.25
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to investigate the anti-inflammatory effects of water extract from Euonymus alatus (Thunb.) Sieb. (EAS) on the RAW 264.7 cells. To evaluate of anti-inflammatory of EAS, we examined the cytokine productions on lipopolysaccharide (LPS)-induced RAW 264.7 cells and also inhibitory mechanisms using Western blot. EAS reduced LPS-induced production of nitric oxide (NO), interleukin (IL)-1b, IL-6, IL-10 and tumor necrosis factor-a (TNF-a) in RAW 264.7 cells. EAS inhibited the activation of mitogen-activated protein kinases (MAPKs) such as p38, extracelluar signal-regulated kinase (ERK 1/2) and c-Jun NH2-terminal kinase (JNK) but not of inhibitory kappa B a (Ik-Ba) degradation in the LPS-stimulated RAW 264.7 cells. In conclusion, EAS down-regulated LPS-induced NO and cytokines production, which could provide a clinical basis.

Keywords

References

  1. Park, W.H., Kim, S.H., Kim, C.H. A new matrix metalloproteinase-9 inhibitor 3,4-dihydroxycinnamic acid (caffeic acid) from methanol extract of Euonymus alatus: isolation and structure determination. Toxicology. 207: 383-390, 2005 https://doi.org/10.1016/j.tox.2004.10.008
  2. Chung, H.S., Jeong, H.J., Kim, J.S., Jeong, S.I., Kim, K.S., Kim, K.S. et al. Activation of inducible nitric oxide synthase by Euonymus alatus in mouse peritoneal macrophages. Clin Chim Acta. 318: 113-120, 2002. https://doi.org/10.1016/S0009-8981(01)00808-7
  3. Jin, U.H., Lee, J.Y., Kang, S.K., Kim, J.K., Park, W.H., Kim, J.G. et al. A phenolic compound, 5-caffeoylquinic acid (chlorogenic acid), is a new type and strong matrix metalloproteinase-9 inhibitor: isolation and identification from methanol extract of Euonymus alatus. Life Sci. 77: 276-279, 2005
  4. Kim, C.H., Kim, D.I., Kwon, C.N., Kang, S.K., Jin, U.H., Suh, S.J. et al. Euonymus alatus (Thunb.) Sieb. induces apoptosis via mitochondrial pathway as prooxidant in human uterine leiomyomal smooth muscle cells. Int J Gynecol Cancer. 16: 843-848, 2006 https://doi.org/10.1111/j.1525-1438.2006.00524.x
  5. Lee, T.K., Lee, J.Y., Kim, D.I., Lee, Y.C., Kim, C.H. Differential regulation of protein kinase C activity by modulating factors and Euonymus alatus (Thunb.) Sieb in human myometrial and uterine leiomyomal smooth muscle cells. Int J Gynecol Cancer. 15: 349-358, 2005 https://doi.org/10.1111/j.1525-1438.2005.15228.x
  6. Park, S.H., Ko, S.K., Chung, S.H. Euonymus alatus prevents the hyperglycemia and hyperlipidemia induced by high-fat diet in ICR mice. J Ethnopharmacol. 102: 326-335, 2005. https://doi.org/10.1016/j.jep.2005.06.041
  7. Seo, U.K., Lee, Y.J., Kim, J.K., Cha, B.Y., Kim, D.W., Nam, K.S. et al. Large-scale and effective screening of Korean medicinal plants for inhibitory activity on matrix metalloproteinase-9. J Ethnopharmacol. 97: 101-106, 2005 https://doi.org/10.1016/j.jep.2004.10.022
  8. Chung, T.W., Moon, S.K., Chang, Y.C., Ko, J.H., Lee, Y.C., Cho, G. et al. Novel and therapeutic effect of caffeic acid and caffeic acid phenyl ester on hepatocarcinoma cells: complete regression of hepatoma growth and metastasis by dual mechanism. FASEB J. 18: 670-681, 2004
  9. Lee, T.K., Kim, D.I., Han, J.Y., Kim, C.H. Inhibitory effects of Scutellaria barbata D. Don. and Euonymus alatus Sieb. on aromatase activity of human leiomyomal cells. Immunopharm Immunotoxicol. 26: 315-327, 2004 https://doi.org/10.1081/IPH-200026840
  10. Cha, B.Y., Park, C.J., Lee, D.G., Lee, Y.C., Kim, D.W., Kim, J.D. et al. Inhibitory effect of methanol extract of Euonymus alatus on matrix metalloproteinase-9. J Ethnopharmacol. 85: 163-167, 2002
  11. Abul, K. Abbas. Cellular and Molecular Immunology 6th edition, Saunders Elsevier. pp 75-97, 2006
  12. Nathan, C., Xie, Q.W. Nitric oxide synthases: roles, tolls and controls. Cell. 78: 915-918, 1994 https://doi.org/10.1016/0092-8674(94)90266-6
  13. Kwqamata, H., Ochiai, H., Mantani, N., terasawa, K. Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW264.7 cells, a murine macrophage cell line. Am J Chin Med. 28: 217-226, 2000 https://doi.org/10.1142/S0192415X0000026X
  14. Lee, B.G., Kim, S.H. Zee, O.P., Lee, K.R., Lee, HY., Han, J.W., Lee, H.W. Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach. Eur J Pharmacol. 406: 301-309, 2000 https://doi.org/10.1016/S0014-2999(00)00680-4
  15. Seo, W.G., Pae, H.O., Oh, G.S., Chai, K.Y., Yun, Y.G., Kwon, T.O., Chung, H.T. Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-and lipopolysaccharide. Gen Pharmacol. 35: 21-28, 2000 https://doi.org/10.1016/S0306-3623(01)00086-6
  16. Chiou, W.F., Chou, C.J., Chen, C.F. Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages. Life Sci. 69: 625-635, 2001 https://doi.org/10.1016/S0024-3205(01)01154-7
  17. Seo, W.G., Pae, H.O., Oh, G.S., Kim, N.Y., Kwon, T.O., Shin, M.K., Chai, K.Y., Chung, H.T. The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW264.7 macrophage. J Ethnophamacol. 76: 119-123, 2001 https://doi.org/10.1016/S0378-8741(01)00220-3
  18. Horwood, N.J., Page, T.H., McDaid, J.P., Palmer, C.D., Campbell, J., Mahon, T., Brennan, F.M., Webster, D., Foxwell, B.M. Bruton's tyrosine kinase is required for TLR2 and TLR4-induced TNF, but not IL-6, production. J Immunol. 176(6): 635-641, 2006
  19. Hirohashi, N., Morrison, D.C. Low-dose lipopolysaccharide (LPS) pretreatment of mouse macrophage modulates LPS-dependent interleukin-6 production in vitro. Infect Immun. 64(3):1011, 1996
  20. Matsuda, H., Morikawa, T., Ando, S., Toguchida, I. and Yoshikawa. M. Structural requirements of flavonoids for nitric oxide production inhibitory activity and mechanism of action. Bioorganic Med. Chem. 11: 1995-2000, 2003 https://doi.org/10.1016/S0968-0896(03)00067-1
  21. Calixto, J.B., Campos, M.M., Otuki, M.F., Santos, A.R. Anti-inflammatory compounds of plant origin. Part II. modulation of pro-inflammatory cytokines, chemokines and adhesion molecules. Planta Med. 70(2):93-103, 2004 https://doi.org/10.1055/s-2004-815483
  22. Swearingen, J.K., Reshetiloff, B., Slattery and S. Zwicker. 'Winged Burning Bush'. Plant Invaders of Mid-Atlantic Natural Areas. National Park Service and U.S. Fish & Wildlife Service. 2002
  23. Huang, K.C. The pharmacology of Chinese Herbs. CRC Press, Boca Raton, FL. pp 343-346, 1993
  24. 전국한의과대학 본초학 공동교재편찬위원회. 본초학. 서울, 영림사, p 720, 2007
  25. Middleton, Jr. E., Kandaswami, C. Effects of flavonoids on immune and inflammatory cell functions. Biochem Pharmacol. 43: 1167-1179, 1992 https://doi.org/10.1016/0006-2952(92)90489-6
  26. Robak, J., Gryglewski, R.J. Flavonoids are scavengers of superoxide anions. Biochem Pharmacol. 37: 837-841, 1988 https://doi.org/10.1016/0006-2952(88)90169-4
  27. la Sala, A., Gadina, M., Kelsall, B.L. G(i)-protein-dependent inhibition of IL-12 production is mediated by activation of the phosphatidylinositol 3-kinase-protein 3 kinase B/Akt pathway and JNK. J. Immunol. 175: 2994-2999, 2005 https://doi.org/10.4049/jimmunol.175.5.2994
  28. Wu, G.H., Zhang, Y.W., Wu, Z.H. Modulation of postoperative immune and inflammatory response by immune-enhancing enteral diet in gastrointestinal cancer patients. World J Gastroenterol. 7: 357-362, 2001 https://doi.org/10.3748/wjg.v7.i3.357
  29. Bryan, N.S. Nitrite in nitric oxide biology: cause or consequence? A systems based review. Free Radic Biol Med. 41: 691-701, 2006 https://doi.org/10.1016/j.freeradbiomed.2006.05.019
  30. Bhattacharyya, A., Pathak, S., Datta, S., Chattopadhyay, S., Basu, J., Kundu, M. Mitogen-activated protein kinases and NF-kappaB regulate H. pylori-mediated IL-8 release from macrophages. Biochem J. 366: 376-382, 2002
  31. Binetruy, B., Smeal, T., Kariu, M. Ha-Ras augments c-Jun activity and stimulates phosphoylation of its activation domain. Nature. 351: 122-127, 1991 https://doi.org/10.1038/351122a0
  32. Garrington, T.P., Johnson, G.L. Organization and regulation of mitogen-activated protein kinase signaling pathways. Curr Opin Cell Biol. 11: 211-218, 1999 https://doi.org/10.1016/S0955-0674(99)80028-3
  33. Seo, J.H., Lim, J.W., Kim, H., Kim, K.H. Helicobacter pylori in a Korean isolate activates mitogen-activated protein kinases. AP-1, and NF-kappaB and induces chemokine expression in gastric epithelial AGS cells. lab Invest. 84: 49-62, 2004 https://doi.org/10.1038/labinvest.3700010
  34. Lee, A.K., Sung, S.H., Kim, Y.C., Kim, S.G. Inhibition of lipopolysaccharide-inducible nitiric oxide synthase.TNF-$\alpha$ and COX-2 expression by suchinone effects on I-${\kappa}B{\alpha}$ phosphorylation. C/EBP and AP-1 activation. British J.Phamacol. 139: 11-20, 2003 https://doi.org/10.1038/sj.bjp.0705231
  35. Meng, F., Lowell, CA. Lipopolysaccharide(LPS)-induced macrophage activation and signal transduction in the absence of Src-family kinase Hck, Fgr, and Lyn. J Exp Med. 185(9):1661, 1997 https://doi.org/10.1084/jem.185.9.1661