Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Inhibitory Effects of Piperine on the Production of Nitric Oxide, Interleukin-10 and Interleukine-12 in Murine Peritoneal Macrophages

복강 대식세포에서 피페린의 일산화질소, 인테루킨-10과 인테루킨-12의 억제 효과

  • Bae, Gi-Sang (Department of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Ju-Sung (Department of Circulatory Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Sung, Kang-Keyng (Department of Circulatory Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Park, Sung-Joo (Department of Herbology, College of Oriental Medicine, Wonkwang University)
  • 배기상 (원광대학교 한의과대학 본초학교실) ;
  • 이주성 (원광대학교 한의과대학 심계내과학교실) ;
  • 성강경 (원광대학교 한의과대학 심계내과학교실) ;
  • 박성주 (원광대학교 한의과대학 본초학교실)
  • Published : 2009.04.25
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Abstract

The purpose of this study was to investigate the anti-inflammatory effects and cellular mechanism of piperine on murine peritoneal macrophages. To evaluate the effects of piperine, we examined the production of nitric oxide (NO), interleukin (IL)-10 and IL-12. To investigate inhibitory mechanism of piperine, we examined the MAPKs and Ik-Ba in murine peritoneal macrophages, Piperine itself does not have any cytotoxic effect and reduced lipopolysaccharid (LPS), Poly(I:C), CpG-ODN -induced production of NO, IL-10 and IL-12 in peritoneal macrophages. Piperine inhibited the activation of extracelluar signal-regulated kinase (ERK 1/2) and c-Jun NH2-terminal kinase (JNK 1/2) not the activation of p38 and the degradation of inhibitory kappa B a (Ik-Ba) in the LPS-stimulated murine peritoneal macrophages.ln conclusion, Piperine down-regulated LPS-induced production of NO, IL-10 and IL-12, which could provide a clinical basis for anti-inflammatory properties of piperine.

Keywords

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