Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Continentalic from Aralia Continentalis on Growth Inhibition and Apoptosis Induction in Human Leukemia HL-60 Cells

독활 유래 Continentalic Acid가 인간 백혈병 HL-60 세포의 성장억제와 아포토시스 유도에 미치는 영향

  • Kim, Sun-Young (Department of Physiology, Center for Healthcare Technology Development, Chonbuk National University Medical School) ;
  • Jeong, Seung-Il (Jeonju Biomaterials Institute) ;
  • Kim, Sung-Zoo (Department of Physiology, Center for Healthcare Technology Development, Chonbuk National University Medical School) ;
  • Shim, Jae-Suk (Imsilsangyak Farming Association) ;
  • Jang, Seon-Il (Jeonju University Atopy & Health Research Institute)
  • 김선영 (전북대학교 의학전문대학 헬스케어사업단 생리학교실) ;
  • 정승일 (전주생물소재연구소) ;
  • 김성주 (전북대학교 의학전문대학 헬스케어사업단 생리학교실) ;
  • 심재석 (임실생약영농조합) ;
  • 장선일 (전주대학교 대체의학대학 대체건강관리학부 건강관리)
  • Published : 2009.12.25
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Abstract

In previous study, we have shown that continentalic acid (CA) isolated from Aralia continentalis induced the growth inhibition and apoptosis in HepG2 cells. In this study, we examine the effects of CA from A. continentalis on growth inhibition and apoptosis induction in human leukemia HL-60 and mouse fibroblast NIH 3T3 cell lines. The results demonstrated that CA decreased cell growth of leukemia HL-60 cells but not human HaCaT keratinocytes, assessed with the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and LDH (lactate dehydrogenase) assay. Flow cytometric analysis of mouse fibroblast cell lines exposed to CA showed that apoptotic cells increased in a time- and dose-dependent manner. Treatment with CA decreased the number of normal cells and increased the number of early apoptotic and late apoptotic cells in a dose-dependent manner. The induction of apoptosis in mouse cell lines by CA was mediated through the activation of caspase-3, Bak, and Bax and the down-regulation of Bcl-2. Our results suggest that CA efficiently induces apoptosis in human leukemia cells.

Keywords

References

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