Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Shigyungbanha-Tang on the Lipopolysaccharide-Induced Acute Lung Injury in Mice

시경반하탕(柴梗半夏湯)이 LPS로 유발된 급성 폐손상에 대한 영향

  • Kim, Ki-Tae (Department of Internal Medicine, College of Oriental Medicine, Semyung University) ;
  • Ko, Heung (Department of Internal Medicine, College of Oriental Medicine, Semyung University)
  • 김기태 (세명대학교 한의과대학 내과학교실) ;
  • 고흥 (세명대학교 한의과대학 내과학교실)
  • Published : 2009.12.25
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the effects of Shigyungbanha-tang(SGT) on the lipopolysaccharide(LPS) induced acute lung injury(ALI) in mice. 1 and 24 h before LPS intratracheal instillation, control group was taken distilled water orally. Treated groups was taken each concentrate SGT(2.5 g/kg, 6.7 g/kg) by orally as same times. Normal group was not instilled with LPS and was taken distilled water. 24 h after LPS intratracheal instillation, lung histology was performed in inflated-fixed lungs in 3 mice of each groups. The other mice of each groups, bronchoalveolar lavege fluids(BALF) was obtained to measure proinflammatory cytokines(TNF-$\alpha$, IL-$1{\beta}$, IL-6) and blood sample was obtained to measure white blood cell(WBC). In vitro, the effect of SGT($100\;ug/m{\ell}$, $500\;ug/m{\ell}$, $1000\;ug/m{\ell}$) on the release of RANTES, TARC induced by TNF-$\alpha$ and IL-4 in human alveolar epithelial cell(A549) was examined. Histopathologically, SGT prevented LPS-induced lung injury. SGT decreased protein, TNF-$\alpha$, IL-$1{\beta}$ and IL-6 according to concentrations. In vitro, $500\;ug/m{\ell}$, $1000\;ug/m{\ell}$ concentrate SGT suppressed the expression of RANTES and TARC on A549 cells. On the basis of these results, SGT had a markedly anti-inflammatory effect in a clinically relevant model of ALI. Nevertheless, further investigations are required to determine the potential clinical usefulness of SGT in the adjunctive therapy of ALI.

Keywords

References

  1. 전국한의과대학 폐계내과학교실. 동의폐계내과학. 서울, 나도, pp 72-74, 90, 192-199, 2007
  2. 대한내과학회 해리슨내과학 편집위원회. Harrison's Principles of Internal medicine 16th edition. 서울, MIP, pp 1737-1740, 2006
  3. 서울대학교의과대학 내과학교실. 임상 내과학(I). 서울, 고려의학, pp 1076-1089, 2004
  4. 이상민, 유철규. 급성호흡곤란증후군. 대한내과학회지 68(5): 476-486, 2005
  5. Kahdi, F., Udobi, E.D., Childs Karim Touijer. Acute Respiratory Distress Syndrome. AMERICAN FAMILY PHYSICIAN 67(20):315-322, 2003
  6. 김성은, 이영만, 박원학. 내독소로 유도된 급성폐손상에서 PLA2의 억제제인 Rutin의 효과. Korean J. Electron Microscopy 34(1):31-42, 2004
  7. Vadas, P., Browning, J., Edelson, J., Pruzanski, W. Extracellular phospholipase A2 expression and inflammation. Journal of Lipid Mediators 8: 1-8, 1993
  8. 김선영, 김창환, 김태규, 김희제, 서영훈, 성정석, 엄현석, 이기현 외. 면역학 제2판. 서울, 라이프사이언스, pp 223-273, 2006
  9. 윤호주. 급성 폐손상에서 cytokine의 역할. 대한내과학회지 54(4):544-549, 1999
  10. 권 현, 정승기, 이형구. 시경청폐탕과 시경반하탕이 Xylene으로 인한 흰쥐의 폐부종에 미치는 영향. 경희의학, 5(3):337-345, 1989
  11. 이승언, 신조영, 이시형. LPS로 유도한 복강대식세포에서 IκBa 분해억제에 의한 柴梗半夏湯의 항염증효과. 대한한방내과학회지 28(3):442-452, 2007
  12. 김민희, 조승제, 송준섭, 노창수, 정헌종. 내독소 투여로 유발된 폐손상에서 표면활성제 투여 방법이 IL-1$\beta$와 IL-6치에 미치는 효과. 대한주산의학회지 15(2):154-163, 2004
  13. Lewis, J.F., McCaig, L. Aerosolized versus instilled exogenous surfactant in nonuniform pattern of lung injury. Am Rev Respir Dis 148: 1187-1193, 1993 https://doi.org/10.1164/ajrccm/148.5.1187
  14. Articas, A., Bernard, G.R., Carlet, J., Dreyfuss, D., Gattinoni, L., Hudson, L., Lamy, M., Marinni, J.O., Matthay, M.A., Pinsky, M.R., Spracc, R., Suter, P.M. and the Consensus Committee. The American-European Consensus Conference on ARDS, Part 2. Am J Respir Crit Care Med 157: 1337-1347, 1998
  15. Katsumi Shimoda, Hiroaki Nakazawa, Maret, G., Traber, Daniel, L. Traber, Motohiro Nozaki. Plasma and tissue vitamin E depletion in sheep with burn and smoke inhalation injury. burns 34: 1137-1141, 2008 https://doi.org/10.1016/j.burns.2008.01.015
  16. Mandana, M., Mojtaba, M., Ali, M., Mohammad, S.S., Atabak, N., Mohammad, R.K., Molook, H., Mohammad, H.G. The role of glutathione-S-transferase polymorphisms on clinical outcome of ALI/ARDS patient treated with N-acetylcysteine. Respiratory Medicine 1: 1-8, 2008 https://doi.org/10.1016/j.rmedc.2008.04.010
  17. Chia Chou Yeh, Shang Jyh Kao, Chih Che Lin, Shulhn Der Wang, Ching Ju Liu, Shung Te Kao. The immunomodulation of endotoxin-induced acute lung injury by hesperidin In vivo and In vitro. Life Sciences 80(20):1821-1831, 2007 https://doi.org/10.1016/j.lfs.2007.01.052
  18. M. Bachofen, E.R. Weibel. Structural alterations of lung parenchyma in the adult respiratory distress syndrome. Clinics in Chest Medicine 31: 35-56, 1982
  19. Hyungug Cho. Germanium increases alveolar macrophage engulfment of apoptotic neutrophils in acute lung injury induced by endotoxin. Korean J. Electron Microscopy 35(4):1-7, 2005
  20. 김성호, 김희택. 황금이 A549 세포주에서 TNF-a 및 IL-4로 유도된 chemokines에 미치는 영향. 한방안이비인후피부과학회지 20(2):108-115, 2007
  21. Alam, R., et al. RANTES is a chemotactic and activating factor for human eosinophils. Journal of Immunology 150: 3442-3448, 1993
  22. Imai, T., et al. The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. Journal of Biological Chemistry 272(23):15036-15042, 1997 https://doi.org/10.1074/jbc.272.23.15036
  23. 郭子光 外. 現代中醫治療學. 四川, 四川科學技術出版社, pp 92-94, 1995
  24. 李梴. 醫學入門. 서울, 大星文化社, p 208, 1984
  25. 윤용갑. 동의방제와 처방해설. 서울, 의성당, pp 639-692, 2002
  26. 신원용, 현민경, 정병무, 최은영, 윤철호, 정지천. 노인성 폐렴환자 1례에 대한 증례보고. 대한한방내과학회지 26(1):229-235, 2005
  27. 이경진, 김재홍, 문상관, 조기호, 배형섭, 이경섭. 뇌졸중의 폐렴합병증에 대한 시경반하탕의 치험1예. 경희의학 15(1)107-112, 1999
  28. 전국한의과대학 본초학교실. 본초학. 서울, 영림사, pp 136-541, 2000
  29. Chia Chou Yeh, Chih Che Lin, Shulhn Der Wang, Che Ming Hung, Ming Hsien Yeh, Ching Ju Liu, Shung Te Kao. Protective and immunomodulatory effect of Gingyo-san in a murine model of acute lung inflammation. Journal of Ethnopharmacology 111(2):418-426, 2007 https://doi.org/10.1016/j.jep.2006.12.014
  30. Behnia, R., Molteni, A., Waters, C.M., Panos, R.J., Ward, W.F., Schnaper, H.W. Early markers of ventilator-induced lung injury in rats. Ann Clin Lab Sci 26: 437-450, 1996
  31. Xiao Su, Yuanlin Song, Jinjun Jiang, Chunxue Bai. The role of aquaporin-1(AQP1) expression in a murine model of lipopolysaccharide-induced acute lung injury. Respiratory Physiology & Neurobiology 142(1):1-11, 2004 https://doi.org/10.1016/j.resp.2004.05.001
  32. 정진홍, 신경철. Interleukin-1으로 유도된 흰쥐 급성폐손상에서 Aerosolized Vitamin-E의 전처치 효과. 영남의대학술지 24(2):365-372, 2007
  33. Hierholzer, C., Kalff, J.C., Omert, L., Tsukada, K., Loeffert, J.E., Watkins, S.C., et al. Interleukin-6 prodection in hemorrhagic shock is accompanied by neutrophil recruitment and lung injury. Am J Physiol 275: 611-621, 1998
  34. Van der Poll, T., Keogh, C.V., Guirao, X., Buurman, W.A., Kopf, M., Lowry, S.F. Interleukin-6 gene-deficient mice show impaired defense against pneumococcal pneumonia. J Infect Dis 176(2):439-444, 1997 https://doi.org/10.1086/514062