Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Combined Treatment with Coptidis Rhizoma Extract and Arsenic Trioxide Enhanced Apoptosis through Diverse Pathways in H157 Cells

  • Youn, Myung-Ja (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University) ;
  • Kim, Yun-Ha (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University) ;
  • Kim, Hyung-Jin (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University) ;
  • Song, Je-Ho (Department of Beauty Design, Wonkwang University) ;
  • Jeon, Ho-Sung (School of Oriental Medicine, Wonkwang University) ;
  • Yu, Dong-Hee (School of Oriental Medicine, Wonkwang University) ;
  • Sul, Jeong-Dug (College of Sport Science, Chung-Ang University) ;
  • So, Hong-Seob (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University) ;
  • Park, Rae-Kil (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University)
  • Published : 2009.12.25
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Abstract

Coptidis rhizoma (huanglian) is an herb that is widely used in traditional Chinese medicine that has recently been shown to possess anticancer activity. However, the molecular mechanism underlying the anticancer effects of this herb is poorly understood. In this study, we investigated the anticancer activity of a combination of CR extract and arsenic trioxide, as well as the apoptotic pathway associated with its mechanism of action in human lung cancer H157 cells. Combined treatment of H157 cells with CR extract and arsenic trioxide resulted in significant apoptotic death. In addition, combined treatment with CR extract and arsenic trioxide acted in concert to induce a loss of mitochondrial membrane potential (${\Delta}{\Psi}$), the release of cytochrome c from mitochondria, and an increase in the expression of pro-apoptotic p53 and Bax protein, which resulted in activation of caspases and apoptosis. CR extract combined with arsenic trioxide also increased the lipid peroxidation, mRNA expression of DR4 and DR5 and caspase-8 activity. These data indicate that combined treatment with CR extract and arsenic trioxide enhanced apoptotic cell death in H157 cells through diverse pathways, including mitochondrial dysfunction and death receptors, particularly DR4 and DR5. Thus, this treatment may be an effective from of chemotherapy.

Keywords

References

  1. Chen, Y.J., Shiao, M.S., Lee, S.S., Wang, S.Y. Effect of Cordyceps sinensis on the proliferation and differentiation of human leukemic U937 cells. Life Science 60: 2349-2359, 1997 https://doi.org/10.1016/S0024-3205(97)00291-9
  2. Ikezoe, T., Chen, S.S., D., Heber, H., Taguchi, H., Koeffler, P. Baicalin is a major component of PCSPES which inhibits the proliferation of human cancer cells via apoptosis and cell cycle arrest. Prostate 49: 285-292, 2001 https://doi.org/10.1002/pros.10024
  3. Yang, S., Chen, J., Guo, Z., Xu, X.M., Wang, L., Pei, X.F., Yang, J., Underhill, C.B., Zhang, L. Triptolide inhibits the growth and metastasis of solid tumors. Mol Cancer Ther. 2(1):65-72, 2003
  4. Xu, J.T., Wang, L.Q., Xu, B. Research development of Coptidis Chinese. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 26(6):704-707, 2004
  5. Franzblau, S.G. and Cross, C. Comparative in vitro antimicrobial activity of Chinese medicinal herbs. Journal of Ethnopharmacology 15: 279-288, 1986 https://doi.org/10.1016/0378-8741(86)90166-2
  6. Iizuka, N., Miyamoto, K., Okita, K., Tangoku, A., Hayashi, H., Yosino, S., Abe, T., Morioka, T., Hazama, S., Oka, M. Inhibitory effect of Coptidis Rhizoma and berberine on the proliferation of human esophageal cancer cell lines. Cancer Letters 148: 19-25, 2000a https://doi.org/10.1016/S0304-3835(99)00264-5
  7. Iizuka, N., Miyamoto, K., Hazama, S., Yoshino, S., Yoshimura, K., Okita, K., Fukumoto, T., Yamamoto, S., Tangoku, A., Oka, M. Anticachectic effects of Coptidis rhizoma, an anti-inflammatory herb, on esophageal cancer cells that produce interleukin-6. Cancer Letters 158: 35-41, 2000b https://doi.org/10.1016/S0304-3835(00)00496-1
  8. Tang, J., Feng, Y., Tsao, S., Wang, N., Curtain, R., Wang, Y. Berberine and Coptidis Rhizoma as novel antineoplastic agents: A review of traditional use and biomedical investigations. Journal of Ethnopharmacology 126: 5-17, 2009 https://doi.org/10.1016/j.jep.2009.08.009
  9. Avvisati, G., Lo Coco, F., Mandelli, F. Acute promyelocytic leukemia: clinical and morphologic features and prognositic factors. Semin Hematology 38: 4-12, 2001 https://doi.org/10.1016/S0037-1963(01)90001-0
  10. Fenaux, P., Chomienne, C., Degos, L. All-trans retinoic acid and chemotherapy in the treatment of acute promyelocytic leukemia. Semin Hematology 38: 13-25, 2001
  11. Chen, Z., Chen, G.Q., Shen, Z.X., Chen, S.J., Wang, Z.Y. Treatment of acute promyelocytic leukemia with arsenic compounds: in vitro and in vivo studies. Semin Hematology 38: 26-36, 2001 https://doi.org/10.1053/shem.2001.20863
  12. Zhang, T., Wang, S.S., Hong, L., Wang, X.L., Qi, Q.H. Arsenic trioxide induces apoptosis of rat hepatocellular carcinoma cells in vivo. Journal of Experimental Clincal Cancer Research 22: 61-68, 2003
  13. Woo, S.Y., Lee, M.Y., Jung, Y.J. Arsenic trioxide inhibits cell growth in SH-SY5Y and SK-N-As nuroblastoma cell line by a different mechanism. Pediatric Hematology & Oncology 23: 231-243, 2006 https://doi.org/10.1080/08880010500506818
  14. Ling, Y.H., Jiang, J.D., Holland, J.F., Perez-Soler, R. Arsenic trioxide produces polymerization of microtubules and mitotic arrest before apoptosis in human tumor cell lines. Molecular Pharmacology 62: 529-538, 2002 https://doi.org/10.1124/mol.62.3.529
  15. Ivanovska, N. and S. Philipov. Study on the anti-inflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids. International Journal of Immunopharmacology 18: 553-561, 1996 https://doi.org/10.1016/S0192-0561(96)00047-1
  16. Youn, M.J., So, H.S., Cho, H.J., Kim, H.J., Kim, Y., Lee, J.H., Sohn, J.S., Kim, Y.K., Chung, S.Y., Park, B. Berberine, a natural product, combined with cisplatin enhanced apoptosis through a mitochondria/caspase-mediated pathway in HeLa cells. Biol. Pharma Bull. 31: 789-795, 2008 https://doi.org/10.1248/bpb.31.789
  17. Ohkawa, H., Ohishi, N., Yagi, K. Reaction of linoleic acid hydro-peroxide with thiobarbituric acid. Journal of Lipid Research 19: 1053-1057, 1978
  18. Ford, M.S., Maggirwar, S.B., Rybak, L.P., Whitworth, C., Ramkumar, V. Expression and function of adenosine receptors in the chinchilla cochlea. Hearing Research 105: 130-140, 1997 https://doi.org/10.1016/S0378-5955(96)00204-3
  19. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry pp 193, 265-283, 1951
  20. Kim, H.R., Kim, E.J., Yang, S.H., Jeong, E.T., Park, C., Lee, J.H., Youn, M.J., So, H.S., Park, R. Trichostatin A induces apoptosis in lung cancer cells via simultaneous activation of the death receptor-mediated and mitochondrial pathway Experimental Molecular Medicine 31: 616-624, 2006
  21. Sheridan, J.P., Marsters, S., Pitti, R.M., Gurney, A., Skubatch, M., Baldwin, D., Ramakrishnan, L., Gray, C.L., Baker, K., Wood, W.I. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 277: 818-821, 1997 https://doi.org/10.1126/science.277.5327.818
  22. Wiley, S.R., K. Schooley, P.J., Smolak, W.S., Din, C.P., Huang, J.K., Nicholl, G.R., Sutherland, T.D., Smith, C.R., Smith, C.A. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 3: 673-683, 1995 https://doi.org/10.1016/1074-7613(95)90057-8
  23. Pitti, R.M., Marsters, S.A., Ruppert, S., Donahue, C.J., Moore, A., Ashkenazi, A. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. Journal of Biological Chemistry 271: 12687-12690, 1996 https://doi.org/10.1074/jbc.271.22.12687
  24. Pan, G., Ni, J., Wei, Y., Yu, G., Gentz, R., Dixit, V.M. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 277: 815-818, 1997 https://doi.org/10.1126/science.277.5327.815
  25. Iizuka, N., Oka, M., Yamamoto, S., Tangoku, A., Miyamoto, K., Miyamoto, T., Uchimura, S., Hamamoto, Y., Okita, K. Identification of common or distinct genes related to antitumor activities of a medicinal herb and its major component by oligonucleotide microarray. International Journal of Cancer 107: 666-672, 2003 https://doi.org/10.1002/ijc.11452
  26. Eom, K.S., Hong, J.M., So, H.S., Park, R., Kim, J.M., Kim, T.Y. Berberine induces G1 arrest and apoptosis in human glioblastoma T98G cells through mitochondrial/caspases pathway. Biol. Pharma Bull 31: 558-562, 2008 https://doi.org/10.1248/bpb.31.558
  27. Meeran, S.M., Katiyae, S.K., Katiyar, S.K. Berberine-induced apoptosis in human prostate cancer cells is initiated by reactive oxygen species generation. Toxicology and Applied Pharmacology 229: 33-43, 2008 https://doi.org/10.1016/j.taap.2007.12.027
  28. Vernhet, L., Allain, N.Le., Vee, M., Morel, F., Guillouzo, A., Fardel, O. Blockage of multidrug resistance-associated proteins potentiates the inhibitory effects of arsenic trioxide on CYP1A1 induction by polycyclic aromatic hydrocarbons. Journal of Pharmacology and Experimental Therapeutics 304: 145-155, 2003 https://doi.org/10.1124/jpet.102.042176
  29. Wang, X., Kong, L., Zhao, J., Yang, P. Arsenic trioxide in the mechanism of drug resistance reversal in MCF-7/ADM cell line of human breast cancer. Zhonghua Zhongliu Zazhi 24: 339-343, 2002
  30. Bennett, M.R. Mechanisms of p53-induced apoptosis. Biochemical & Pharmacology 1: 1089-1095, 1999
  31. Li, P.F., Dietz, R., von Harsdorf, R. p53 regulates mitochondrial membrane potential through reactive oxygen species and induces cytochrome c-independent apoptosis blocked by Bcl-2. EMBO Journal 18: 6027-6036, 1993 https://doi.org/10.1093/emboj/18.21.6027
  32. Oltvai, Z.N., C.L. Milliman and S.J. Korsmeyer. Bcl-2 heterodimerizes in vivo with a conserved homolog, bax, that accelerates programmed cell death. Cell 74: 609-619, 1993 https://doi.org/10.1016/0092-8674(93)90509-O
  33. Israeli, D., Tessler, E., Haupt, Y., Elkeles, A., Wilder, S., Amson, R., Telerman, A., Oren, M. A novel p53-inducible gene, PAG608, encodes a nuclear zinc finger protein whose overexpression promotes apoptosis. EMBO Journal 16: 4384-4392, 1997 https://doi.org/10.1093/emboj/16.14.4384
  34. Sheikh, M.S., Burns, T.F., Huang, Y., Wu, G.S., Amundson, S., Brooks, K.S., Fornace, A.J., El-Deiry, W.S. p53-dependent and -independent regulation of the death receptor KILLER/DR5 gene expression in response to genotoxic stress and tumornecrosis factora. Cancer Research 58: 1593-1598, 1998