The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
권호 표지

Olibanum Extract Inhibits Vascular Smooth Muscle Cell Migration and Proliferation in Response to Platelet-Derived Growth Factor

  • Choi, Ok-Byung (Department of Cosmetic Science, College of Natural Science, Hoseo University) ;
  • Park, Joo-Hoon (Department of Cosmetic Science, College of Natural Science, Hoseo University) ;
  • Lee, Ye-Jin (Department of Cosmetic Science, College of Natural Science, Hoseo University) ;
  • Lee, Chang-Kwon (Department of Physiology, School of Medicine, Konkuk University) ;
  • Won, Kyung-Jong (Department of Physiology, School of Medicine, Konkuk University) ;
  • Kim, Jung-Hwan (Department of Physical Therapy, College of Health & Welfare, Yongin University) ;
  • Lee, Hwan-Myung (Department of Cosmetic Science, College of Natural Science, Hoseo University) ;
  • Kim, Bo-Kyung (Department of Physiology, School of Medicine, Konkuk University)
  • Published : 2009.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Olibanum (Boswellia serrata) has been shown to have anti-inflammatory, anti-arthritic and anticancer effects. This study determined the role of a water extract of olibanum in platelet-derived growth factor (PDGF)-stimulated proliferation and migration of rat aortic smooth muscle cells (RASMCs). PDGF-BB induced the migration and proliferation of RASMCs that were inhibited by olibanum extract in a dose-dependent manner. The PDGF-BB-increased phosphorylation of p38 mitogen-activated protein kinase (MAPK); the heat shock protein (Hsp) 27 was significantly inhibited by the olibanum extract. The effects of PDGF-BB-induced extracellular signal-regulated kinase1/2 was not altered by the olibanum extract. Treatment with olibanum extract inhibited PDGF-BB-stimulated sprout out growth of aortic rings. These results suggest that the water extract of olibanum inhibits PDGF-BB-stimulated migration and proliferation in RASMCs as well as sprout out growth, which may be mediated by the inhibition of the p38 MAPK and Hsp27 pathways.

Keywords

References

  1. Bilato C, Pauly RR, Melillo G, Monticone R, Gorelick-Feldman D, Gluzband YA, Sollott SJ, Ziman B, Lakatta EG, Crow MT. Intracellular signaling pathways required for rat vascular smooth muscle cell migration. Interactions between basic fibroblast growth factor and platelet-derived growth factor. J Clin Invest 96: 1905−1915, 1995 https://doi.org/10.1172/JCI118236
  2. Champagne MJ, Dumas P, Orlov SN, Bennett MR, Hamet P, Tremblay J. Protection against necrosis but not apoptosis by heat-stress proteins in vascular smooth muscle cells: evidence for distinct modes of cell death. Hypertension 33: 906−913, 1999 https://doi.org/10.1161/01.HYP.33.3.906
  3. Chevrier MR, Ryan AE, Lee DY, Zhongze M, Wu-Yan Z, Via CS. Boswellia carterii extract inhibits TH1 cytokines and promotes TH2 cytokines in vitro. Clin Diagn Lab Immunol 12: 575−580, 2005
  4. Dardik A, Yamashita A, Aziz F, Asada H, Sumpio BE. Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin- 1alpha. J Vasc Surg 41: 321−331, 2005 https://doi.org/10.1016/j.jvs.2004.11.016
  5. Gayathri B, Manjula N, Vinaykumar KS, Lakshmi BS, Balakrishnan A. Pure compound from Boswellia serrata extract exhibits anti-inflammatory property in human PBMCs and mouse macrophages through inhibition of TNFalpha, IL-1beta, NO and MAP kinases. Int Immunopharmacol 7: 473−482, 2007 https://doi.org/10.1016/j.intimp.2006.12.003
  6. Geraldes P, Sirois MG, Bernatchez PN, Tanguay JF. Estrogen regulation of endothelial and smooth muscle cell migration and proliferation: role of p38 and p42/44 mitogen-activated protein kinase. Arterioscler Thromb Vasc Biol 22: 1585−1590, 2002 https://doi.org/10.1161/01.ATV.0000035393.11854.6A
  7. Grotendorst GR, Seppä HE, Kleinman HK, Martin GR. Attachment of smooth muscle cells to collagen and their migration toward platelet-derived growth factor. Proc Natl Acad Sci USA 78: 3669−3672, 1981 https://doi.org/10.1073/pnas.78.6.3669
  8. Guay J, Lambert H, Gingras-Breton G, Lavoie JN, Huot J, Landry J. Regulation of actin filament dynamics by p38 map kinase- mediated phosphorylation of heat shock protein 27. J Cell Sci 110: 357−368, 1997
  9. Gupta I, Gupta V, Parihar A, Gupta S, Lüdtke R, Safayhi H, Ammon HP. Effects of Boswellia serrata gum resin in patients with bronchial asthma: results of a double-blind, placebo-controlled, 6-week clinical study. Eur J Med Res 3: 511−514, 1998
  10. Hedges JC, Dechert MA, Yamboliev IA, Martin JL, Hickey E, Weber LA, Gerthoffer WT. A role for p38 MAPK/HSP27 pathway in smooth muscle cell migration. J Biol Chem 274: 24211−24219, 1999 https://doi.org/10.1074/jbc.274.34.24211
  11. Huang MT, Badmaev V, Ding Y, Liu Y, Xie JG, Ho CT. Anti-tumor and anti-carcinogenic activities of triterpenoid, beta-boswellic acid. Biofactors 13: 225−230, 2000 https://doi.org/10.1002/biof.5520130135
  12. Kim B, Kim J, Bae YM, Cho SI, Kwon SC, Jung JY, Park JC, Ahn HY. p38 mitogen-activated protein kinase contributes to the diminished aortic contraction by endothelin-1 in DOCA-salt hypertensive rats. Hypertension 43: 1086−1091, 2004 https://doi.org/10.1161/01.HYP.0000125995.85427.fd
  13. Kimmatkar N, Thawani V, Hingorani L, Khiyani R. Efficacy and tolerability of Boswellia serrata extract in treatment of osteoarthritis of knee-a randomized double blind placebo controlled trial. Phytomedicine 10: 3−7, 2003 https://doi.org/10.1078/094471103321648593
  14. Kozawa O, Tanabe K, Ito H, Matsuno H, Niwa M, Kato K, Uematsu T. Sphingosine 1-phosphate regulates heat shock protein 27 induction by a p38 MAP kinase-dependent mechanism in aortic smooth muscle cells. Exp Cell Res 250: 376−380, 1999 https://doi.org/10.1006/excr.1999.4536
  15. Lee CK, Lee HM, Kim HJ, Park HJ, Won KJ, Roh HY, Choi WS, Jeon BH, Park TK, Kim B. Syk contributes to PDGF-BB- mediated migration of rat aortic smooth muscle cells via MAPK pathways. Cardiovas Res 74: 159−168, 2007 https://doi.org/10.1016/j.cardiores.2007.01.012
  16. Lee HM, Jeon BH, Won KJ, Lee CK, Park TK, Choi WS, Bae YM, Kim HS, Lee SK, Park SH, Irani K, Kim B. Gene transfer of redox factor-1 inhibits neointimal formation: involvement of platelet-derived growth factor-beta receptor signaling via the inhibition of the reactive oxygen species-mediated Syk pathway. Circ Res 104: 219−227, 2009 https://doi.org/10.1161/CIRCRESAHA.108.178699
  17. Lee HM, Kim HJ, Park HJ, Won KJ, Kim J, Shin HS, Park PJ, Kim HJ, Lee KY, Park SH, Lee CK, Kim B. Spleen tyrosine kinase participates in Src-mediated migration and proliferation by PDGF-BB in rat aortic smooth muscle cells. Arch Pharm Res 30: 761−769, 2007 https://doi.org/10.1007/BF02977640
  18. Liu B, Ryer EJ, Kundi R, Kamiya K, Itoh H, Faries PL, Sakakibara K, Kent KC. Protein kinase C-$\delta$ regulates migration and proliferation of vascular smooth muscle cells through the extracellular signal-regulated kinase 1/2. J Vascs Surg 45: 160−168, 2007 https://doi.org/10.1016/j.jvs.2006.09.053
  19. Millette E, Rauch BH, Kenagy RD, Daum G, Clowes AW. Platelet-derived growth factor-BB transactivates the fibroblast growth factor receptor to induce proliferation in human smooth muscle cells. Trends Cardiovasc Med 16: 25−28, 2006 https://doi.org/10.1016/j.tcm.2005.11.003
  20. Nicocia RF, Ottinetti A. Growth of microvessels in serum-free matrix culture of rat aorta. Lab Invest 63: 115−122, 1990
  21. Pearson G, Robinson F, Beers Gibson T, Xu BE, Karandikar M, Berman K, Cobb MH. Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr Rev 22: 153−183, 2001 https://doi.org/10.1210/er.22.2.153
  22. Pompili VJ, Gordon D, San H, Yang Z, Muller DW, Nabel GJ, Nabel EG. Expression and function of a recombinant PDGF B gene in porcine arteries. Arterioscler Thromb Vasc Biol 15: 2254−2264, 1995 https://doi.org/10.1161/01.ATV.15.12.2254
  23. Raines EW, Ross R. Smooth muscle cells and the pathogenesis of the lesions of atherosclerosis. Br Heart J 69: S30−37, 1993 https://doi.org/10.1136/hrt.69.1_Suppl.S30
  24. Robinson MJ, Cobb MH. Mitogen-activated protein kinase pathways. Curr Opin Cell Biol 9: 180−186, 1997 https://doi.org/10.1016/S0955-0674(97)80061-0
  25. Ross R. Atherosclerosis is an inflammatory disease. New Engl J Med 340: 115−126, 1999 https://doi.org/10.1056/NEJM199901143400207
  26. Salinthone S, Ba M, Hanson LJ, Martin JL, Halayko AJ, Gerthoffer WT. Overexpression of human Hsp27 inhibits serum induced proliferation in airway smooth muscle myocytes and confers resistance to hydrogen peroxide cytotoxicity. Am J Physiol Lung Cell Mol Physiol 293: L1194−L1207, 2007 https://doi.org/10.1152/ajplung.00453.2006
  27. Sharma ML, Khajuria A, Kau, A, Singh S, Singh GB, Atal CK. Effect of salai guggal ex-Boswellia serrata on cellular and humoral immune responses and leucocyte migration. Agents Actions 24: 161−164, 1988 https://doi.org/10.1007/BF01968095
  28. Wei CY, Chou YH, Ho FM, Hsieh SL, Lin WW. Signaling pathways of LIGHT induced macrophage migration and vascular smooth muscle cell proliferation. J Cell Physiol 209: 735−743, 2006 https://doi.org/10.1002/jcp.20742
  29. Winking M, Sarikaya S, Rahmanian A, Jödicke A, Böker DK. Boswellic acids inhibit glioma growth: a new treatment option? J Neurooncol 46: 97−103, 2000 https://doi.org/10.1023/A:1006387010528
  30. Y J, Kamath JV, Asad M. Effect of hexane extract of Boswellia serrata oleo-gum resin on chemically induced liver damage. Pak J Pharm Sci 19: 129−133, 2006
  31. Zhan Y, Kim S, Izumi Y, Izumiya Y, Nakao T, Miyazaki H, Iwao H. Role of JNK, p38, and ERK in platelet-derived growth factor-induced vascular proliferation, migration, and gene expression. Arterioscler Thromb Vasc Biol 23: 795−801, 2003 https://doi.org/10.1161/01.ATV.0000066132.32063.F2