Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Dohongsamul-Tang on the Gene Expression of Photothrombotic Ischemia Mouse Model

도홍사물탕(桃紅四物湯)이 광화학적 뇌경색 마우스의 유전자 발현에 미치는 영향

  • Cho, Kwon-Il (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim, Hye-Yoon (Department of Internal Medicine, School of Oriental Medicine, Wonkwang University) ;
  • Ko, Seok-Jae (Department of Internal Medicine, School of Oriental Medicine, Wonkwang University) ;
  • Lee, Seong-Geun (Department of Internal Medicine, School of Oriental Medicine, Wonkwang University) ;
  • Shin, Sun-Ho (Department of Internal Medicine, School of Oriental Medicine, Wonkwang University) ;
  • Moon, Byung-Soon (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 조권일 (원광대학교 한의학전문대학원 제3의학과) ;
  • 김혜윤 (원광대학교 한의과대학 내과학교실) ;
  • 고석재 (원광대학교 한의과대학 내과학교실) ;
  • 이성근 (원광대학교 한의과대학 내과학교실) ;
  • 신선호 (원광대학교 한의과대학 내과학교실) ;
  • 문병순 (원광대학교 한의학전문대학원 제3의학과)
  • Published : 2009.06.25
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Abstract

The water extract of Dohongsamul-Tang(DHSMT) has been traditionally used to stroke and brain injuries in Oriental Medicine. The present study was designed to investigate the effects of DHSMT on the gene expression profile of cerebral infarction by cDNA microarray in photothrombotic ischemia mouse model. Photothrombotic ischemia was induced in stereotactically held male BALB/c mice using rose bengal and cold light. MRI was performed 24 hours after inducing photothrombosis using 1.5 T MRI and 47 mm surface coil to obtain T2-weighted, and contrast-enhanced images. After MRI test, animal was sacrificed and the brain sections were stained for hematoxylin and eosin and immunohistochemistry. MRI and histological analysis revealed that lesion of thrombotic ischemia was well induced in the cortex with the evidence of biological courses of infarction. The target area of thrombotic infarction was 1 mm anterior to bregma and 3 mm lateral to midline with 2 mm in diameter, which were decreased by administration of DHSMT. To assess gene expression pattern of cerebral infarction, mRNA was isolated and reacted with microarray chip(Agilant's DNA Microarray 44K). Scatter and MA plot analysis were performed to clustering of each functional genes. M value [M=log2(R/G), A={log2(R ${\times}$ G)}/2] was between -0.5 and +0.5 with 40% difference. After pretreatment with DHSMT, the expression levels of mRNA of many genes involved in various signaling pathway such as apoptosis, cell cycle, cell proliferation, response to oxidative stress, immune response, angiogenesis, and inflammatory cytokine were markedly inhibited in photothrombotic ischemia lesion compared to the control group. These results suggest that DHSMT prevent ischemic death of brain on photothrombotic ischemia model of mice through modulation of gene expression at the transcriptional level.

Keywords

References

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