Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-inflammatory Effects of Gelidium amansii in RAW 264.7 Macrophages

RAW 264.7 대식세포에서 Gelidium amansii의 항염증 효과

  • Choi, Won-Sik (Department of Biotechnology, Soonchunhyang University) ;
  • Kim, Young-Sun (Department of Biotechnology, Soonchunhyang University) ;
  • Lee, Sang-Hyun (Department of Oriental Medical Prescription, College of Oriental Medicine, Wonkwang University) ;
  • Chai, Kyu-Yun (Division of Nanobiochemistry, Wonkwang University) ;
  • Lee, Young-Haeng (Division of Nanobiochemistry, Wonkwang University)
  • 최원식 (순천향대학교 생명공학과) ;
  • 김영선 (순천향대학교 생명공학과) ;
  • 이상현 (원광대학교 한의과대학 방제학교실) ;
  • 채규윤 (원광대학교 생명나노화학부) ;
  • 이영행 (원광대학교 생명나노화학부)
  • Published : 2009.06.25
Download PDF
⟨ Previous Next ⟩

Abstract

In order to verify the anti-inflammatory effects of Gelidium amansii, RAW264.7 macrophages were incubated with the extract of 70% ethanol solution (Ex), and activated with the endotoxin lipopolysaccharide (LPS). Ex inhibited the expression of the pro-inflammatory enzymes, including inducible nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2), and the production of iNOS-mediated NO and COX-2-mediated prostglandin $E_2$ ($PGE_2$) production in a dose-dependent manner. Ex also reduced the release of the pro-inflammatory cytokines, including tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-1${\beta}$ (IL-1${\beta}$) and IL-6 in LPS-activated macrophages, The observed anti-inflammatory effects of Ex was associated with inactivation of the nuclear factor ${\kappa}B$ (NF-${\kappa}B$) that mediates the induction of iNOS, COX-2, TNF-${\alpha}$, IL-1${\beta}$, and IL-6. Further studies showed that Ex inactivated NF-${\kappa}B$ through inhibition of phosphorylation of the inhibitory ${\kappa}B$ ($l{\kappa}B$), Taken together, these results suggest that Gelidium amansii exerts anti-inflammatory effects by inhibiting the expression of pro-inflammatory enzymes and the secretion of pro-inflammatory cytokines via inactivation of NF-${\kappa}B$ and/or $l{\kappa}B$.

Keywords

References

  1. Singh, S.B,, Pelaez, F., Biodiversity, A. Chemical diversity and drug discovery. Prog. Drug Res. 65: 141, 143-174, 2008
  2. Awad, N.E. Biologically active steroid from the green alga Ulva lactuca, Phytother. Res. 14: 641-643, 2000 https://doi.org/10.1002/1099-1573(200012)14:8<641::AID-PTR668>3.0.CO;2-R
  3. Ganovski, K., Shipochliev, T., Bratova, K. Anti-inflammatory action of extracts from marine algae collected in the area of Burgas seacoast, Vet. Med. Nauki. 16: 54-61, 1979
  4. Guzman, S., Gato, A., Calleja, J.M. Antiinflammatory, analgesic and free radical scavenging activities of the marine microalgae Chlorella stigmatophora and Phaeodactylum tricornutum, Phytother. Res. 15: 224-230, 2001 https://doi.org/10.1002/ptr.715
  5. Li, X., Kwak, O.S., Jin, H. A survey of medicinal seaweed resources in Liaodong Peninsula, Chin. Marine Pharm. Sci. 51: 50-54, 1994
  6. Allen, L.A., Aderem, A. Mechanisms of phagocytosis. Curr. Opin. Immunol. 8: 36-40, 1996 https://doi.org/10.1016/S0952-7915(96)80102-6
  7. Murtaugh, M.P., Baarsch, M.J., Zhou, Y., Scamurra, R.W., Lin, G. Inflammatory cytokines in animal health and disease. Vet. Immunol. Immunopathol. 54: 45-55, 1996 https://doi.org/10.1016/S0165-2427(96)05698-X
  8. Murakami, A., Ohigashi, H. Targeting NOX, iNOS and COX-2 in inflammatory cells: chemoprevention using food phytochemicals. Int. J. Cancer. 121(11):2357-2363, 2007 https://doi.org/10.1002/ijc.23161
  9. van der Meide, P.H., Schellekens, H. Cytokines and the immune response. Biotherapy. 8: 243-249, 1996 https://doi.org/10.1007/BF01877210
  10. Pae, H.O., Lee, Y.C., Chung, H.T. Heme oxygenase-1 and carbon monoxide: emerging therapeutic targets in inflammation and allergy. Recent Pat. Inflamm. Allergy Drug Discov. 2: 159-165, 2008 https://doi.org/10.2174/187221308786241929
  11. Namazi, M.R. Cetirizine and allopurinol as novel weapons against cellular autoimmune disorders. Int. Immunopharmacol. 4: 349-353, 2004 https://doi.org/10.1016/j.intimp.2004.01.022
  12. Pande, V., Ramos, M.J. NF-kB in human disease: current inhibitors and prospects for de novo structure based design of inhibitors. Curr. Med. Chem. 12: 357-374, 2005 https://doi.org/10.2174/0929867053363180
  13. Karpuzoglu, E., Ahmed, S.A. Estrogen regulation of nitric oxide and inducible nitric oxide synthase (iNOS) in immune cells: implications for immunity, autoimmune diseases, and apoptosis. Nitric Oxide. 15: 177-186, 2006 https://doi.org/10.1016/j.niox.2006.03.009
  14. Tinker, A.C., Wallace, A.V. Selective inhibitors of inducible nitric oxide synthase: potential agents for the treatment of inflammatory diseases? Curr. Top. Med. Chem. 6: 77-92, 2006 https://doi.org/10.2174/156802606775270297
  15. Guzik, T.J., Korbut, R., Adamek-Guzik, T. Nitric oxide and superoxide in inflammation and immune regulation. J. Physio. Pharmacol. 54: 469-487, 2003
  16. Kleinert, H., Schwarz, P.M., Forstermann, U. Regulation of the expression of inducible nitric oxide synthase. Biol. Chem. 384: 1343-1364, 2003 https://doi.org/10.1515/BC.2003.152