Inhibitory Effects of Ethanol Extract of Modified Yukgunga-tang on Obesity and Hyperlipidemia in Rats Induced by High Fat Diet

육군자탕가감방 에탄올 추출물의 비만 및 고지혈증 유도 흰쥐에 대한 억제효능

  • Park, Jung-Hyun (Department of Pathology, College of Oriental Medicinen & Institute of Oriental Medicine, Kyunghee University) ;
  • Kang, Hee (Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Ahn, Kwang-Seok (Department of Pathology, College of Oriental Medicinen & Institute of Oriental Medicine, Kyunghee University) ;
  • Shim, Bum-Sang (Department of Pathology, College of Oriental Medicinen & Institute of Oriental Medicine, Kyunghee University) ;
  • Kim, Sung-Hoon (Cancer Preventive Material Development Research Center) ;
  • Choi, Seung-Hoon (Department of Pathology, College of Oriental Medicinen & Institute of Oriental Medicine, Kyunghee University) ;
  • Ahn, Kyoo-Seok (Department of Pathology, College of Oriental Medicinen & Institute of Oriental Medicine, Kyunghee University)
  • 박정현 (경희대학교 한의과대학 병리학교실 & 경희대학교 한의학 연구소) ;
  • 강희 (경희대학교 동서의학대학원 동서의과학) ;
  • 안광석 (경희대학교 한의과대학 병리학교실 & 경희대학교 한의학 연구소) ;
  • 심범상 (경희대학교 한의과대학 병리학교실 & 경희대학교 한의학 연구소) ;
  • 김성훈 (암예방소재개발연구센터) ;
  • 최승훈 (경희대학교 한의과대학 병리학교실 & 경희대학교 한의학 연구소) ;
  • 안규석 (경희대학교 한의과대학 병리학교실 & 경희대학교 한의학 연구소)
  • Published : 2009.06.25

Abstract

This experimental study was designed to investigate the inhibitory effects of ethanol extract of modified Yukgunja-tang(mYGJT) on high-fat diet-induced obesity and hyperlipidemia in Sprague-Dawley rats, Animals were divided into normal, control, mYGJT(100 mg/kg and 200 mg/kg) treated groups. Obesity with hyperlipidemia was induced by high fat diet treatment for 6 weeks. mYGJT was given to the amimals by oral gavage for 4 weeks, starting at the high-fat diet regimen, The effect of mYGJT on the differentiation of 3T3 L1 adipocytes in vitro and serological paramamters for obesity and hyperlipidemia in vivo were evaluated, mYGJT significnatly inhibited the differentiation of 3T3 L1 adipocytes in a concentration dependent manner. mYGJT treatment siginficantly reduced body weight, abdominal and epididymal fat weight, and FER(Food Efficiency Ratio) compared with control group in a dose dependent manner. It also signficantly inhibited the levels of serum total lipid, triglyceride, phospholipid, total cholesterol, LDL, AI(Atherosclerosis Index) and returned the serum HDL to normal. Total lipids, triglycerides and cholesterols in the liver, as well as malondialdehyde(MDA) and hydroxy radical in the serum were significantly reduced. However, superoxide dismutase(SOD) activity was significantly increased in mYGJT treated group compared with control group. Finally, mYGJT treatment signficantly decreased the MDA and protein carbonyl concentrations of the hepatic homogenate but signficantly increased the activities of SOD, GSH-Px and Catalase. Taken together, these results suggest that mYGJT can be clinically useful in inhibiting high-fat diet-induced obesity and hyperlipidemia.

Keywords

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