The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Electrophysiological Characterization of AMPA and NMDA Receptors in Rat Dorsal Striatum

  • Jeun, Seung-Hyun (Department of Pharmacology, Medical Research Center for Cell Death Disease Research Center, The Catholic University of Korea) ;
  • Cho, Hyeong-Seok (Department of Pharmacology, Medical Research Center for Cell Death Disease Research Center, The Catholic University of Korea) ;
  • Kim, Ki-Jung (Department of Pharmacology, Medical Research Center for Cell Death Disease Research Center, The Catholic University of Korea) ;
  • Li, Qing-Zhong (Department of Pharmacology, Medical Research Center for Cell Death Disease Research Center, The Catholic University of Korea) ;
  • Sung, Ki-Wug (Department of Pharmacology, Medical Research Center for Cell Death Disease Research Center, The Catholic University of Korea)
  • Published : 2009.06.30
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Abstract

The striatum receives glutamatergic afferents from the cortex and thalamus, and these synaptic transmissions are mediated by ${\alpha}$-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) and N-methyl D-aspartate (NMDA) receptors. The purpose of this study was to characterize glutamate receptors by analyzing NMDA/AMPA ratio and rectification of AMPA and NMDA excitatory postsynaptic currents (EPSCs) using a whole-cell voltage-clamp method in the dorsal striatum. Receptor antagonists were used to isolate receptor or subunit specific EPSC, such as (DL)-2-amino-5-phosphonovaleric acid (APV), an NMDA receptor antagonist, ifenprodil, an NR2B antagonist, CNQX, an AMPA receptor antagonist and IEM-1460, a GluR2-lacking AMPA receptor blocker. AMPA and NMDA EPSCs were recorded at - 70 and + 40 mV, respectively. Rectification index was calculated by current ratio of EPSCs between + 50 and - 50 mV. NMDA/AMPA ratio was 0.20${\pm}$0.05, AMPA receptor ratio of GluR2-lacking/GluR2-containing subunit was 0.26${\pm}$0.05 and NMDA receptor ratio of NR2B/NR2A subunit was 0.32${\pm}$0.03. The rectification index (control 2.39${\pm}$0.27) was decreased in the presence of both APV and combination of APV and IEM-1460 (1.02${\pm}$0.11 and 0.93${\pm}$0.09, respectively). These results suggest that the major components of the striatal glutamate receptors are GluR2-containing AMPA receptors and NR2A-containing NMDA receptors. Our results may provide useful information for corticostriatal synaptic transmission and plasticity studies.

Keywords

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