The Korean Journal of Physiology and Pharmacology

  • 제13권3호
  • /
  • Pages.251-256
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  • 2009
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  • 1226-4512(pISSN)
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  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
권호 표지

Bis Is Involved in Glial Differentiation of PI9 Cells Induced by Retinoic Acid

  • Yoon, Jung-Sook (Departments of Biomedical Science, The Catholic University of Korea) ;
  • Lee, Mun-Yong (Departments of Anatomy, Graduate School, College of Medicine, The Catholic University of Korea) ;
  • Lee, Jae-Seon (Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences) ;
  • Park, Chan-Sun (Bioindustry Research Center, Korea Research Institute Bioscience and Biotechnology) ;
  • Youn, Ho-Joong (Internal Medicine, The Catholic University of Korea) ;
  • Lee, Jeong-Hwa (Biochemistry, College of Medicine, The Catholic University of Korea)
  • 발행 : 2009.06.30
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초록

Previous observations suggest that Bis, a Bcl-2-binding protein, may playa role the neuronal and glial differentiation in vivo. To examine this further, we investigated Bis expression during the in vitro differentiation of P19 embryonic carcinoma cells induced by retinoic acid (RA). Western blotting and RT-PCR assays showed that Bis expression was temporarily decreased during the free floating stage and then began to increase on day 6 after the induction of differentiation. Double immunostaining indicated that Bis-expressing cells do not express several markers of differentiation, including NeuN, MAP-2 and Tuj-1. However, some of the Bis-expressing cells also were stained with GFAP-antibodies, indicating that Bis is involved glial differentiation. Using an shRNA strategy, we developed bis-knock down P19 cells and compared them with control P19 cells for the expression of NeuroD, Mash-1 and GFAP during RA-induced differentiation. Among these, only GFAP induction was significantly attenuated in Pl9-dnbis cells and the population showing GFAP immunoreactivity was also decreased. It is noteworthy that distribution of mature neurons and migrating neurons was disorganized, and the close association of migrating neuroblasts with astrocytes was not observed in P19-dnbis cells. These results suggest that Bis is involved in the migration-inducing activity of glial cells.

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