Asian-Australasian Journal of Animal Sciences

  • 제23권5호
  • /
  • Pages.640-648
  • /
  • 2010
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Molecular Cloning and mRNA Expression of the Porcine Insulin-responsive Glucose Transporter (GLUT4)

  • Zuo, Jianjun (College of Animal Science, South China Agricultural University) ;
  • Dai, Fawen (College of Animal Science, South China Agricultural University) ;
  • Feng, Dingyuan (College of Animal Science, South China Agricultural University) ;
  • Cao, Qingyun (College of Animal Science, South China Agricultural University) ;
  • Ye, Hui (College of Animal Science, South China Agricultural University) ;
  • Dong, Zemin (College of Animal Science, South China Agricultural University) ;
  • Xia, Weiguang (College of Animal Science, South China Agricultural University)
  • 투고 : 2009.06.03
  • 심사 : 2009.11.13
  • 발행 : 2010.05.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Insulin-responsive glucose transporter 4 (GLUT4) is a member of the glucose transporter family and mainly presents in skeletal muscle and adipose tissue. To clarify the molecular structure of porcine GLUT4, RACE was used to clone its cDNA. Several cDNA clones corresponding to different regions of GLUT4 were obtained by amplifying reverse-transcriptase products of total RNA extracted from Landrace porcine skeletal muscles. Nucleotide sequence analysis of the cDNA clones revealed that porcine GLUT4 cDNA was composed of 2,491 base pairs with a coding region of 509 amino acids. The deduced amino acid sequence was over 90% identical to human, rabbit and cattle GLUT4. The tissue distribution of GLUT4 was also examined by Real-time RT-PCR. The mRNA expression abundance of GLUT4 was heart>liver, skeletal muscle and brain>lung, kidney and intestine. The developmental expression of GLUT4 and insulin receptor (IR) was also examined by Real-time RT-PCR using total RNA extracted from longissimus dorsi (LM), semimembranosus (SM), and semitendinosus (SD) muscle of Landrace at the age of 1, 7, 30, 60 and 90 d. It was shown that there was significant difference in the mRNA expression level of GLUT4 in skeletal muscles of Landrace at different ages (p<0.05). The mRNA expression level of IR also showed significant difference at different ages (p<0.05). The developmental change in the mRNA expression abundance of GLUT4 was similar to that in IR, and both showed a higher level at birth and 30 d than at other ages. However, there was no significant tissue difference in the mRNA expression of GLUT4 or IR (p>0.05). These results showed that the nucleotide sequence of the cDNA clones was highly identical with human, rabbit and cattle GLUT4 and the developmental change of GLUT4 mRNA in skeletal muscles was similar to that of IR, suggesting that porcine GLUT4 might be an insulin-responsive glucose transporter. Moreover, the tissue distribution of GLUT4 mRNA showed that GLUT4 might be an important nutritional transporter in porcine skeletal muscles.

키워드

참고문헌

  1. Diaz, M., E. Capilla and J. V. Planas. 2007. Physiological regulation of glucose transporter (GLUT4) protein content in brown trout (Salmo trutta) skeletal muscle. J. Exp. Biol. 210(13):2346-2351 https://doi.org/10.1242/jeb.002857
  2. Ferguson, D. M., B. L. Daly, G. E. Gardner and R. K. Tume. 2008. Effect of glycogen concentration and form on the response to electrical stimulation and rate of post-mortem glycolysis in ovine muscle. Meat Sci. 78(3):202-210 https://doi.org/10.1016/j.meatsci.2007.06.003
  3. Gaster, M., A. Handberg, H. Beck-Nielsen and H. D. Schroder. 2000. Glucose transporter expression in human skeletal muscle fibers. Am. J. Physiol. Endocrinol. Metab. 279(3):E529-538
  4. Gray, S., B. S. Stonestreet, S. Thamotharan, G. B. Sadowska, M. Daood, J. Watchko and S. U. Devaskar. 2006. Skeletal muscle glucose transporter protein responses to antenatal glucocorticoids in the ovine fetus. J. Endocrinol. 189(2):219-229 https://doi.org/10.1677/joe.1.06589
  5. Hall, J. R., C. E. Short and W. R. Driedzic. 2006. Sequence of Atlantic cod (Gadus morhua) GLUT4, GLUT2 and GPDH: developmental stage expression, tissue expression and relationship to starvation-induced changes in blood glucose. J. Exp. Biol. 209:4490-4502 https://doi.org/10.1242/jeb.02532
  6. He, A. B., X. J. Liu, L. Z. Liu, Y. S. Chang and F. Fang. 2007. How many signals impinge on GLUT4 activation by insulin. Cell. Signal. 19(1):1-7 https://doi.org/10.1016/j.cellsig.2006.05.018
  7. He, J., M. Thamotharan and S. U. Devaskar. 2003. Insulin-induced translocation of facilitative glucose transporters in fetal/neonatal rat skeletal muscle. Am. J. Physiol. Regul. Integr. Comp. Physiol. 284(4):R1138-1146 https://doi.org/10.1152/ajpregu.00560.2002
  8. Heled, Y., Y. Dror, D. S. Moran, T. Rosenzweig, S. R. Sampson, Y. Epstein and J. Meyerovitch. 2005. Physical exercise increases the expression of TNF[alpha] and GLUT 1 in muscle tissue of diabetes prone Psammomys obesus. Life Sci. 77(23):2977-2985 https://doi.org/10.1016/j.lfs.2005.05.033
  9. Hou, J. C. and J. E. Pessin. 2007. Ins (endocytosis) and outs (exocytosis) of GLUT4 trafficking. Cur. Opin. Cell Biol. 19(4):466-473 https://doi.org/10.1016/j.ceb.2007.04.018
  10. Humphrey, B. D., C. B. Stephensen, C. C. Calvert and K. C. Klasing. 2004. Glucose and cationic amino acid transporter expression in growing chickens (Gallus gallus domesticus). Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol. 138(4):515-525 https://doi.org/10.1016/j.cbpb.2004.06.016
  11. Joost, H. G., G. I. Bell, J. D. Best, M. J. Birnbaum, M. J. Charron, Y. T. Chen, H. Doege, D. E. James, H. F. Lodish, K. H. Moley, J. F. Moley, M. Mueckler, S. Rogers, A. Schurmann, S. Seino and B. Thorens. 2002. Nomenclature of the GLUT/SLC2A family of sugar/polyol transport facilitators. Am. J. Physiol. Endocrinol. Metab. 282(4):E974-976
  12. Kitamura, T., Y. Kitamura, J. Nakae, A. Giordano, S. Cinti, C. R. Kahn, A. Efstratiadis and D. Accili. 2004. Mosaic analysis of insulin receptor function. J. Clin. Invest. 113(2):209-219
  13. Knott, R. M., G. Grant, S. Bardocz, A. Pusztai, de Carvalho AFFU and J. E. Hesketh. 1992. Alterations in the level of insulin receptor and GLUT-4 mRNA in skeletal muscle from rats fed a kidney bean (Phaseolus vulgaris) diet. Int. J. Biochem. 24(6):897-902 https://doi.org/10.1016/0020-711X(92)90094-H
  14. Le, K. A., D. Faeh, R. Stettler, C. Debard, E. Loizon, H. Vidal, C. Boesch, E. Ravussin and L. Tappy. 2008. Effects of four-week high-fructose diet on gene expression in skeletal muscle of healthy men. Diabetes Metab. 34(1):82-85 https://doi.org/10.1016/j.diabet.2007.08.004
  15. Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods 25(4):402-408 https://doi.org/10.1006/meth.2001.1262
  16. Manso Filho, H. C., K. H. McKeever, M. E. Gordon, H. E. C. Costa and M. Watford. 2007. Novel findings regarding Glut-4 expression in adipose tissue and muscle in horses - A preliminary report. The Veterinary Journal 174(3):565 https://doi.org/10.1016/j.tvjl.2006.10.022
  17. Saltiel, A. R. and J. E. Pessin. 2002. Insulin signaling pathways in time and space. Trends Cell Biol. 12(2):65-71 https://doi.org/10.1016/S0962-8924(01)02207-3
  18. Scheffler, T. L. and D. E. Gerrard. 2007. Mechanisms controlling pork quality development: The biochemistry controlling postmortem energy metabolism. Meat Sci. 77(1):7-16 https://doi.org/10.1016/j.meatsci.2007.04.024
  19. Tonack, S., N. Ramin, S. Garimella, R. Rao, P. B. Seshagiri, B. Fischer and A. Navarrete Santos. 2009. Expression of glucose transporter isoforms and the insulin receptor during hamster preimplantation embryo development. Ann. Anat. - Anatomischer Anzeiger. 191(5):485-495 https://doi.org/10.1016/j.aanat.2009.06.002
  20. Walker, P. S., T. Ramlal, V. Sarabia, U. M. Koivisto, P. J. Bilan, J. E. Pessin and A. Klip. 1990. Glucose transport activity in L6 muscle cells is regulated by the coordinate control of subcellular glucose transporter distribution, biosynthesis, and mRNA transcription. J. Biol. Chem. 265(3):1516-1523
  21. Watson, R. T. and J. E .Pessin. 2007. GLUT4 translocation: The last 200 nanometers. Cell. Signal. 19:2209-2217 https://doi.org/10.1016/j.cellsig.2007.06.003
  22. Zhao, F. Q. and A. F. Keating. 2007. Functional properties and genomics of glucose transporters. Curr. Genomics 8(2):113-128 https://doi.org/10.2174/138920207780368187