동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제24권6호
  • /
  • Pages.950-955
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  • 2010
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

천정기보단(天精氣保丹)의 자외선에 의한 세포 손상 억제 효과

Protective Effect of Cheonjeongkibo-Dan UV-Induced Cellular Damage in Human Dermal Fibroblast

  • 이강태 (코리아나화장품 송파기술연구소) ;
  • 박시준 (코리아나화장품 송파기술연구소) ;
  • 이정로 (코리아나화장품 송파기술연구소) ;
  • 이광식 (코리아나화장품 송파기술연구소) ;
  • 김대성 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문연자 (원광대학교 한의과대학 해부학교실) ;
  • 이건국 (코리아나화장품 송파기술연구소) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Lee, Ghang-Tai (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Park, Si-Jun (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Lee, Jung-No (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Lee, Kwang-Sik (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Kim, Dae-Sung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of Anatomy, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Kun-Kuk (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 투고 : 2010.11.06
  • 심사 : 2010.12.09
  • 발행 : 2010.12.25
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초록

In this study, we prepared CheonJeongKiBo-Dan(7 oriental medicinal plants, 7OMP: Astragalus Membranaceus root, Panax Ginseng root, Glycyrrhiza Glabra (licorice) root, Schizandra Chinensis fruit, Polygonatum Odoratum, Rehmannia Glutinosa root, Paeonia Albiflora root) by extracting them in one reactor and studied its efficacies on skin. UV irradiation has been suggested as a major cause of photoaging in skin. In order to investigate protective effects against UV-B induced cellular damage, 7OMP was extracted with 70% ethanol and dissolved in DMSO. The protective effect was detected by MTT assay, reactive oxygen species (ROS) generation, phosphorylation of ATR and p53 in human dermal fibroblast cell system after UV-B irradiation. 7OMP reduced UV-B-induced cellular damage in HDFs cells, and inhibited ROS generation. UV-B-induced toxicity accompanying ROS production and the resultant DNA damage are responsible for activation of ATR, p53 and Bad. In this study, 7OMP hampered phosphorylations of ATR and p53 in human dermal fibroblasts. Therefore, 7OMP may be protective against UV-induced skin photoaging.

키워드

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