Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Artemisiae Argi Folium Fermented with Lactobacillus Pentosus and Saccharomyces Cerevisiae on TNF-${\alpha}$ Production in RAW 264.7 and HepG2 Cells

유산균 발효 애엽과 효모균발효 애엽 물추출물의 종양괴사인자-알파 생성촉진효과

  • Kim, Youn-Sub (College of Oriental Medicine, Kyungwon University) ;
  • Park, Wan-Su (College of Oriental Medicine, Kyungwon University)
  • Received : 2010.08.23
  • Accepted : 2010.10.19
  • Published : 2010.12.25
Download PDF
⟨ Previous Next ⟩

Abstract

Tumor necrosis factor-alpha (TNF-${\alpha}$) is a major mediator of immuno-inflammatory activity. The purpose of this study is to investigate whether TNF-${\alpha}$ productions of mouse macrophage RAW 264.7 and human hepatocyte HepG2 are modulated by Artemisiae argi Folium water extract (AW), Lactobacillus pentosus-fermented Artemisiae argi Folium water extract (AFL), and Saccharomyces cerevisiae-fermented Artemisiae argi Folium water extract (AFS) for 3 h of incubation. Effect of AW on cell viability of HepG2 was also investigated. TNF-${\alpha}$ productions were measured by Enzyme-Linked Immnunosorbent Assay method and cell viability was measured by MTT assay. Both AFL and AFS significantly increased TNF-${\alpha}$ productions of RAW 264.7 at the concentration of 50, 100, and 200 ${\mu}g$/mL (p<0.05). Also, AFL and AFS significantly increased TNF-${\alpha}$ productions of HepG2 at the concentration of 50, 100, and 200 ${\mu}g$/mL (p<0.05). AW significantly increased TNF-${\alpha}$ production of HepG2 at the concentration of 100 and 200 ${\mu}g$/mL (p<0.05). AW did not show any cytotoxicity on HepG2 cells for 3 h. These results suggest that AFL, AFS, and AW have the immune-enhancing property related with its increasing effect on TNF-${\alpha}$ production of macrophage and hepatocyte.

Keywords

References

  1. 전국한의과대학 공동교재편찬위원회. 본초학. 서울, 영림사, pp 405-407, 1991.
  2. 허 준. 대역동의보감. 하동, 동의보감출판사, p 2180, 2005.
  3. 박완수. Gallic acid 등으로 유발된 대식세포 내 hydrogen peroxide 생성억제에 대한 유산균발효애엽 추출물의 영향. 동의생리병리학회지 23(2):438-442, 2009.
  4. 박완수, 김도훈. 고삼과 애엽의 발효 혼합물이 에탄올과 니코틴으로 유발된 마우스 대식세포 내 hydrogen peroxide 생성 감소에 미치는 영향. 동의생리병리학회지 22(5):1293-1298, 2008.
  5. 박완수. EtOH 등의 독성물질에 대한 효모균발효애엽 추출물의 간세포보호효과. 동의생리병리학회지 24(2):284-289, 2010.
  6. 박완수. EtOH 등의 독성물질에 대한 유산균발효애엽 추출물의 간세포보호효과. 동의생리병리학회지 24(3):457-462, 2010.
  7. 박완수. 마우스 대식세포(RAW 264.7)에 대한 艾葉물추출물의 생리활성 연구. 동의생리병리학회지 22(4):815-820, 2008.
  8. 한효상. 애엽 발효 추출물의 세포독성과 면역활성에 관한 연구. 경원대학교 대학원 박사학위논문, 2008.
  9. 조수인, 김형우, 이근지. 동백 발효 추출물 단기 투여의 활성에 대한 연구. 대한본초학회지 21(2):55-62, 2006.
  10. Locksley, R.M., Killeen, N., Lenardo, M.J. The TNF and TNF receptor superfamilies: integrating mammalian biology. Cell 104(4):487-501, 2001. https://doi.org/10.1016/S0092-8674(01)00237-9
  11. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 65(1-2):55-63, 1983. https://doi.org/10.1016/0022-1759(83)90303-4
  12. Yoon, S.B., Lee, Y.J., Park, S.K., Kim, H.C., Bae, H., Kim, H.M. , Ko, S.G. , Choi , H.Y. , Oh, M.S. , Park, W. Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages. J Ethnopharmacol. 125(2):286-290, 2009. https://doi.org/10.1016/j.jep.2009.06.027
  13. Kim, M.J., Kim, D.H., Lee, K.W., Yoon, D.Y., Surh, Y.J. Jaceosidin induces apoptosis in ras-transformed human breast epithelial cells through generation of reactive oxygen species. Ann NY Acad Sci. 1095: 483-495, 2007. https://doi.org/10.1196/annals.1397.052
  14. Jeong, M.A., Lee, K.W., Yoon, D.Y., Lee, H.J. Jaceosidin, a pharmacologically active flavone derived from Artemisia argyi, inhibits phorbol-ester-induced upregulation of COX-2 and MMP-9 by blocking phosphorylation of ERK-1 and -2 in cultured human mammary epithelial cells. Ann NY Acad Sci. 1095: 458-466, 2007. https://doi.org/10.1196/annals.1397.049
  15. Lee, H.G., Yu, K.A., Oh, W.K., Baeg, T.W., Oh, H.C., Ahn, J.S., Jang, W.C., Kim, J.W., Lim, J.S., Choe, Y.K., Yoon, D.Y. Inhibitory effect of jaceosidin isolated from Artemisiaargyi on the function of E6 and E7 oncoproteins of HPV 16. J Ethnopharmacol. 98(3):339-343, 2005. https://doi.org/10.1016/j.jep.2005.01.054
  16. Adams, M., Efferth, T., Bauer, R. Activity-guided isolation of scopoletin and isoscopoletin, the inhibitory active principles towards CCRF-CEM leukaemia cells and multi-drug resistant CEM/ADR5000 cells, from Artemisia argyi. Planta Med. 72(9):862-864, 2006. https://doi.org/10.1055/s-2006-947165
  17. Ryan, J.G., Kastner, D.L. Fevers, genes, and innate immunity. Curr Top Microbiol Immunol. 321: 169-184, 2008. https://doi.org/10.1007/978-3-540-75203-5_8
  18. Bennett, M.K., Kirk, C.J. Development of proteasome inhibitors in oncology and autoimmune diseases. Curr Opin Drug Discov Devel. 11: 616-625, 2008.