Toxicological Research

  • 제26권1호
  • /
  • Pages.15-19
  • /
  • 2010
  • /
  • 1976-8257(pISSN)
  • /
  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지
DOI QR코드

DOI QR Code

Identification of Differentially Expressed Genes in Human Mesenchymal Stem Cell-Derived Neurons

  • Heo, Ji-Hye (Department of Biomedical Science, College of Health Sciences, Korea University) ;
  • Cho, Kyung-Jin (Department of Biomedical Science, College of Health Sciences, Korea University) ;
  • Choi, Dal-Woong (Department of Environmental Health, College of Health Sciences, Korea University) ;
  • Kim, Suhng-Wook (Department of Biomedical Science, College of Health Sciences, Korea University)
  • 발행 : 2010.03.04
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Mesenchymal stem cells (MSCs) have greater potential for immediate clinical and toxicological applications, due to their ability to self-renew, proliferate, and differentiate into a variety of cell types. To identify novel candidate genes that were specifically expressed during transdifferentiation of human MSCs to neuronal cells, we performed a differential expression analysis with random priming approach using annealing control primer-based differential display reverse transcription-polymerase chain reaction approach. We identified genes for acyl-CoA thioesterase, tissue inhibitor of metalloproteinases-1, brain glycogen phosphorylase, ubiquitin C-terminal hydrolase and aldehyde reductase were up-regualted, whereas genes for transgelin and heparan sulfate proteoglycan were down-regulated in MSC-derived neurons. These differentially expressed genes may have potential role in regulation of neurogenesis. This study could be applied to environmental toxicology in the field of testing the toxicity of a chemical or a physical agent.

키워드

참고문헌

  1. Aust, L., Devlin, B., Foster, S.J., Halvorsen, Y.D., Hicok, K., du Laney, T., Sen, A., Willingmyre, G.D. and Gimble, J.M. (2004). Yield of human adipose-derived adult stem cells from liposuction aspirates. Cytotherapy, 6, 7-14. https://doi.org/10.1080/14653240310004539
  2. Bianco, P., Riminucci, M., Gronthos, S. and Robey, P.G. (2001). Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells, 19, 180-192. https://doi.org/10.1634/stemcells.19-3-180
  3. Cheng, N., Janumyan, Y.M., Didion, L., Van Hofwegen, C., Yang, E. and Knudson, C.M. (2004). Bcl-2 inhibition of T-cell proliferation is related to prolonged T-cell survival. Oncogene, 23, 3770-3780. https://doi.org/10.1038/sj.onc.1207478
  4. Cho, K.J., Trzaska, K.A., Greco, S.J., McArdle, J., Wang, F.S., Ye, J.H. and Rameshwar, P. (2005). Neurons derived from human mesenchymal stem cells show synaptic transmission and can be induced to produce the neurotransmitter substance P by interleukin-1 alpha. Stem Cells, 23, 383-391. https://doi.org/10.1634/stemcells.2004-0251
  5. Davila, J.C., Cezar, G.G., Thiede, M., Strom, S., Miki, T. and Trosko, J. (2004). Use and application of stem cells in toxicology. Toxicol. Sci., 79, 214-223. https://doi.org/10.1093/toxsci/kfh100
  6. Deans, R.J. and Moseley, A.B. (2000). Mesenchymal stem cells: biology and potential clinical uses. Exp. Hematol., 28, 875-884. https://doi.org/10.1016/S0301-472X(00)00482-3
  7. Filip, S., English, D. and Mokry, J. (2004). Issues in stem cell plasticity. J. Cell. Mol. Med., 8, 572-577. https://doi.org/10.1111/j.1582-4934.2004.tb00483.x
  8. Greco, S.J., Zhou, C., Ye, J.H. and Rameshwar, P. (2007). An interdisciplinary approach and characterization of neuronal cells transdifferentiated from human mesenchymal stem cells. Stem Cells Dev., 16, 811-826. https://doi.org/10.1089/scd.2007.0011
  9. Hwang, I.T., Kim, Y.J., Kim, S.H., Kwak, C.I., Gu, Y.Y. and Chun, J.Y. (2003). Annealing control primer system for improving specificity of PCR amplification. BioTechniques, 35, 1180-1184.
  10. Jackson, P. and Thompson, R.J. (1981). The demonstration of new human brain-specific proteins by high-resolution two-dimensional polyacrylamide gel electrophoresis. J. Neurol. Sci., 49, 429-438. https://doi.org/10.1016/0022-510X(81)90032-0
  11. Jiang, Y., Jahagirdar, B.N., Reinhardt, R.L., Schwartz, R.E., Keene, C.D., Ortiz-Gonzalez, X.R., Reyes, M., Lenvik, T., Lund, T., Blackstad, M., Du, J., Aldrich, S., Lisberg, A., Low, W.C., Largaespada, D.A. and Verfaillie, C.M. (2002). Pluripotency of mesenchymal stem cells derived from adult marrow. Nature, 418, 41-49. https://doi.org/10.1038/nature00870
  12. Kim, Y.J., Kwak, C.I., Gu, Y.Y., Hwang, I.T. and Chun, J.Y. (2004). Annealing control primer system for identification of differentially expressed genes on agarose gels. BioTechniques, 36, 424-430.
  13. Kocher, A.A., Schlechta, B., Gasparovicova, A., Wolner, E., Bonaros, N. and Laufer, G. (2007). Stem cells and cardiac regeneration. Transpl. Int., 20, 731-746. https://doi.org/10.1111/j.1432-2277.2007.00493.x
  14. Korecka, J.A., Verhaagen, J. and Hol, E.M. (2007). Cell-replacement and gene-therapy strategies for Parkinson's and Alzheimer's disease. Regen. Med., 2, 425-446. https://doi.org/10.2217/17460751.2.4.425
  15. Lerou, P.H. and Daley, G.Q. (2005). Therapeutic potential of embryonic stem cells. Blood Rev., 19, 321-331. https://doi.org/10.1016/j.blre.2005.01.005
  16. Lock, L.T. and Tzanakakis, E.S. (2007). Stem/Progenitor cell sources of insulin-producing cells for the treatment of diabetes. Tissue Eng., 13, 1399-1412. https://doi.org/10.1089/ten.2007.0047
  17. Miura, M., Gronthos, S., Zhao, M., Lu, B., Fisher, L.W., Robey, P.G. and Shi, S. (2003). SHED: stem cells from human exfoliated deciduous teeth. Proc. Natl. Acad. Sci. U.S.A., 100, 5807- 5812. https://doi.org/10.1073/pnas.0937635100
  18. Nedivi, E., Hevroni, D., Naot, D., Israeli, D. and Citri, Y. (1993). Numerous candidate plasticity-related genes revealed by differential cDNA cloning. Nature, 363, 718-722. https://doi.org/10.1038/363718a0
  19. Ould-yahoui, A., Tremblay, E., Sbai, O., Ferhat, L., Bernard, A., Charrat, E., Gueye, Y., Lim, N.H., Brew, K., Risso, J.J., Dive, V., Khrestchatisky, M. and Rivera, S. (2009). A new role for TIMP-1 in modulating neurite outgrowth and morphology of cortical neurons. PLoS One, 4, e8289. https://doi.org/10.1371/journal.pone.0008289
  20. Silani, V. and Corbo, M. (2004). Cell-replacement therapy with stem cells in neurodegenerative diseases. Curr. Neurovasc Res., 1, 283-289. https://doi.org/10.2174/1567202043362243
  21. Toma, J.G., Akhavan, M., Fernandes, K.J., Barnabe-Heider, F., Sadikot, A., Kaplan, D.R. and Miller, F.D. (2001). Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat. Cell Biol., 3, 778-784. https://doi.org/10.1038/ncb0901-778
  22. Yamada, J., Kuramochi, Y., Takagi, M. and Suga, T. (2004). Expression of acyl-CoA hydrolase in the developing mouse brain. Neurosci. Lett., 355, 89-92. https://doi.org/10.1016/j.neulet.2003.10.049