The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Intestinal Absorption of Fibrinolytic and Proteolytic Lumbrokinase Extracted from Earthworm, Eisenia andrei

  • Yan, Xiang Mei (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Kim, Chung-Hyo (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Lee, Chul-Kyu (Central Research Institute, Shin Poong Pharm. Co. Ltd.) ;
  • Shin, Jang-Sik (Central Research Institute, Shin Poong Pharm. Co. Ltd.) ;
  • Cho, Il-Hwan (Central Research Institute, Shin Poong Pharm. Co. Ltd.) ;
  • Sohn, Uy-Dong (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
  • Received : 2010.03.04
  • Accepted : 2010.04.12
  • Published : 2010.04.30
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Abstract

To investigate the intestinal absorption of a fibrinolytic and proteolytic lumbrokinase extracted from Eisenia andrei, we used rat everted gut sacs and an in situ closed-loop recirculation method. We extracted lumbrokinase from Eisenia andrei, and then raised polyclonal antibody against lumbrokinase. Fibrinolytic activity and proteolytic activity in the serosal side of rat everted gut sacs incubated with lumbrokinase showed dose- and time-dependent patterns. Immunological results obtained by western blotting serosal side solution using rat everted gut sacs method showed that lumbrokinase proteins between 33.6 and 54.7 kDa are absorbed mostly by the intestinal epithelium. Furthermore, MALDI- TOF mass spectrometric analysis of plasma fractions obtained by in situ recirculation method confirmed that lumbrokinase F1 is absorbed into blood. These results support the notion that lumbrokinase can be absorbed from mucosal lumen into blood by oral administration.

Keywords

References

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