동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제24권3호
  • /
  • Pages.463-469
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  • 2010
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

지질다당체유도 BV2세포손상에 대한 상백피 추출물의 항염증작용

Anti-inflammatory Action of Extract of Mori Cortex against Lipopolysaccharide-induced BV2 Microglia

  • 박신형 (동의대학교 한의과대학 병리학교실) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 엄현섭 (동의대학교 한의과대학 병리학교실) ;
  • 지규용 (동의대학교 한의과대학 병리학교실)
  • Park, Shin-Hyung (Department of Pathology, College of Oriental Medicine, Dongeui University) ;
  • Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University) ;
  • Eom, Hyun-Sup (Department of Pathology, College of Oriental Medicine, Dongeui University) ;
  • Chi, Gyoo-Yong (Department of Pathology, College of Oriental Medicine, Dongeui University)
  • 투고 : 2010.05.17
  • 심사 : 2010.06.12
  • 발행 : 2010.06.25
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초록

This research is performed to obtain positive evidences of Mori cortex, a kind of oriental medicinal herbs, in the cellular levels. The extracts of M. cortex have shown anti-inflammatory effects against cutaneous inflammation and clinical effects on pulmonary asthma and congestion in oriental medicine. Thus BV2 cells were chosen because microglia are considered as the main immunocompetent cells in the central nervous system. Lipopolysaccharide (LPS)-induced microglial activation of cultured BV2 cells and subsequent release of nitric oxide (NO) and Prostaglandin E2 (PGE2) were effectively suppressed by methylene chloride extract of Morus alba L. (MEMA). From the inflammation-mediated mRNA and protein analyses, we showed that inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-$1{\beta}$ (IL-$1{\beta}$) and tumor necrosis alpha (TNF-${\alpha}$) induced by LPS were markedly decreased by MEMA treatment. From the observation of nuclear factor-kB (NF-${\kappa}B$) which is controlling and mediating inflammation through COX-2 and iNOS, there showed that p65, a subunit of NF-${\kappa}B$, was increased in nuclear and $I{\kappa}B$, a competitor of NF-${\kappa}B$, was recovered in cytosol after MEMA treatment. These are corresponding with results of iNOS, COX-2, IL-$1{\kappa}$ and TNF-${\alpha}$, and confirm some suppressive effect against transcriptional activation of NF-${\kappa}B$. In conclusion, the anti-inflammatory action of M. cortex against BV2 microglia cells is expected to protect nerve tissues through suppression of neuronal inflammation in various neurodegenerative diseases.

키워드

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