Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Codonopsis Lanceolata Inhibits Inflammation through Regulation of MAPK in LPS-stimulated RAW264.7 cells

LPS로 유도된 RAW264.7 염증모델에서 MAPK 조절에 의한 양유(羊乳)의 항염증효과

  • Kim, Bum-Hoi (Department of Anatomy, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University) ;
  • Lee, Yong-Tae (Department of Oriental Physiology, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University) ;
  • Kang, Kyung-Hwa (Department of Oriental Physiology, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University)
  • 김범회 (동의대학교 한의과대학 해부학교실 & 한의학 연구소) ;
  • 이용태 (동의대학교 한의과대학 생리학교실 & 한의학 연구소) ;
  • 강경화 (동의대학교 한의과대학 생리학교실 & 한의학 연구소)
  • Received : 2010.01.12
  • Accepted : 2010.01.28
  • Published : 2010.02.25
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Abstract

Codonopsis Lanceolata (CL) has been widely used in Oriental medicine for treatment of chronic inflammatory diseases, such as bronchitis, cough, and spasm; however, the mechanism of its anti-inflammatory activity has not been clarified. In this study, therefore, we investigated the inhibitory effect of CL on LPS-induced inflammation. The effect of CL was analyzed by ELISA, RT-PCR and Western blotting in LPS-stimulated RAW264.7 cells. We found that CL suppressed not only the mRNA expression of pre-inflammatory cytokines, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2, but also the phosphorylation of ERK1/2, JNK1/2 and p38 MAPK. These results suggest that CL exerts an anti-inflammatory effect through the regulation of the mitogen-activated protein kinases (MAPK) pathway, thereby decreasing production of pre-inflammatory cytokines, NO, and PGE2.

Keywords

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