Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Garlic Extracts with Extraction Conditions on Antioxidant and Anticancer Activity

추출조건에 따른 마늘 추출물의 항산화 및 항암활성 효과

  • Kim, Hae-Ja (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Han, Choong-Ho (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim, Nan-Young (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Eun-Kyoung (College of Oriental Medicine, Wonkwang University) ;
  • Lee, Ki-Nam (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Cho, Hwa-Eun (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi, Yun-Hee (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Chong, Myong-Soo (College of Oriental Medicine, Wonkwang University)
  • 김혜자 (원광대학교 한의학전문대학원) ;
  • 한중호 (원광대학교 한의학전문대학원) ;
  • 김난영 (원광대학교 한의학전문대학원) ;
  • 이은경 (원광대학교 한의과대학) ;
  • 이기남 (원광대학교 한의학전문대학원) ;
  • 조화은 (원광대학교 한의학전문대학원) ;
  • 최윤희 (원광대학교 한의학전문대학원) ;
  • 정명수 (원광대학교 한의과대학)
  • Received : 2009.11.13
  • Accepted : 2009.12.30
  • Published : 2010.02.25
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Abstract

The purpose of this study was to investigate the effect of garlic(Allium sativum L.) extracts with extraction conditions on antioxidant and anticancer activity. The extracts prepared for garlic by hot temperature extraction (HG), low temperature extraction (LG), UMPM extraction (UG), fermentation (FG) and black garlic hot temperature (BG) method. Content of total polyphenol compound was the BG higher than other extracts. The EDA (electron donating ability) and SOD-like activity was increased in dose-dependent manners, and the activity of BG and UM was significantly higher than LG and FG. We examined cytotoxicity, nitric oxide production of Raw 264.7 cell and inhibition of HT 1080 cell by MTT assay. All extracts does not have any toxic effects in macrophages(Raw 264.7). And UG inhibited the production of nitrite in Raw 264.7 cells activated with LPS. The antitumor effects of LG and UG on HT 1080 cell was indicated a significantly inhibition activity. These results suggested that UG (UMPM extraction of garlic) have activities of antioxidant, anticancer effects.

Keywords

References

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