Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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The Efficacy of β-cyclodextrin/polyethyleneimine/silk Fibroin Hydrogel in Healing Burnt Wound

사이클로덱스트린/폴리에틸렌이민/실크 피브로인 수화겔의 화상치유효능

  • Seo, Seung Ree (Division of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Mi Sun (Division of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Jin-Chul (Division of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University)
  • 서승리 (강원대학교 바이오산업공학부 생물소재공학전공) ;
  • 이미선 (강원대학교 바이오산업공학부 생물소재공학전공) ;
  • 김진철 (강원대학교 바이오산업공학부 생물소재공학전공)
  • Received : 2011.05.26
  • Accepted : 2011.08.22
  • Published : 2011.12.10
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Abstract

In this study, we investigated the efficacy of ${\beta}$-cyclodextrin (${\beta}CD$) hydrorogel containing silk fibroin (SF) on healing burnt wound. Tosyl ${\beta}CD$ was conjugated to polyethyleneimine (PEI) using epichlorohydrin (EPI) as a cross-linker. The ${\beta}CD/PEI/SF$ hydrogel was applied on the back of mouse and then the efficacy of hydrogel was compared with both positive control group and negative control group. There was no wound healing efficacy showed neither in the drug loaded ${\beta}CD/PEI/SF$ hydrogel group nor in the drug unloaded ${\beta}CD/PEI/SF$ hydrogel group. On the other hand, in the positive control group, a significant reduction of the wound size after the usage of OTC hydrorogel was obtained. The burn-healing histological result showed a similar phenomenon. After hematoxylin-eosin staining the skin induced by burning, and the epithelial growth observed in the dermis, the efficacy of ${\beta}CD/PEI/SF$ hydrogel in healing burnt wound could not be clearly identified.

이 연구는 화상이 유발된 mouse에 사이클로덱스트린/폴리에틸렌이민/실크 피브로인이 함유된 수화겔을 처치하여 화상 치유효능을 평가한 실험이다. 상처치유 효능을 갖는 것으로 알려진 silk fibroin (SF)을 함유한 polyethyleneimine (PEI)/${\beta}$-cyclodextrin (${\beta}CD$) conjugate를 epichlorohydrin (EPI)로 가교시켜 ${\beta}CD/PEI/SF$ 수화겔을 제조하였고, 이를 화상이 유발된 mouse에 처치하여 일반 거즈만 처치한 음성대조군과 상용화되어 있는 상처치유 제품(OTC)을 처치한 양성대조군과 비교해 시간에 따른 화상치유 경과를 지켜보았다. 약물이 로딩되지 않은 ${\beta}CD/PEI/SF$ 수화겔과 약물이 로딩된 ${\beta}CD/PEI/SF$ 수화겔을 처치한 동물 실험군의 화상치유경과는 육안으로 관찰 시, 음성대조군과 비교했을 때에 큰 차이가 없었고 오히려 상처가 악화되는 경향을 보였다. 반면 OTC 연고를 처치한 양성대조군에서는 상처의 크기가 눈에 띄게 줄어들었다. H&E staining을 통하여 진피 층에서의 상피재생을 확인한 결과 또한 ${\beta}CD/PEI/SF$ 수화겔의 화상치유에 대한 효능이 뚜렷하게 나타나지 않았다.

Keywords

Acknowledgement

Supported by : 강원대학교

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