The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Chronic Opium Treatment Can Differentially Induce Brain and Liver Cells Apoptosis in Diabetic and Non-diabetic Male and Female Rats

  • Asiabanha, Majid (Department of Biochemistry, Faculty of Medicine, Qazvin University of Medical Sciences) ;
  • Asadikaram, Gholamreza (Department of Biochemistry and Physiology Research Center, Kerman University of Medical Sciences) ;
  • Rahnema, Amir (Department of Pathology, Faculty of Medicine, Rafsanjan University of Medical Sciences) ;
  • Mahmoodi, Mehdi (Department of Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences) ;
  • Hasanshahi, Gholamhosein (Department of Hematology, Faculty of Medicine, Rafsanjan University of Medical Sciences) ;
  • Hashemi, Mohammad (Department of Biochemistry, Faculty of Medicine, Zahedan University of Medical Sciences) ;
  • Khaksari, Mohammad (Department of Physiology and Physiology Research Center, Kerman University of Medical Sciences)
  • Received : 2011.06.07
  • Accepted : 2011.11.10
  • Published : 2011.12.30
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Abstract

It has been shown that some opium derivatives promote cell death via apoptosis. This study was designed to examine the influence of opium addiction on brain and liver cells apoptosis in male and female diabetic and non-diabetic Wistar rats. This experimental study was performed on normal, opium-addicted, diabetic and diabetic opium-addicted male and female rats. Apoptosis was evaluated by TUNEL and DNA fragmentation assays. Results of this study showed that apoptosis in opium-addicted and diabetic opium-addicted brain and liver cells were significantly higher than the both normal and diabetic rats. In addition, we found that apoptosis in brain cells of opium-addicted and diabetic opium-addicted male rats were significantly higher than opium-addicted and diabetic opium-addicted female, whereas apoptosis in liver cells of opium-addicted and diabetic opium-addicted female rats were significantly higher than opium-addicted and diabetic opium-addicted male. Overall, these results indicate that opium probably plays an important role in brain and liver cells apoptosis, therefore, leading neurotoxicity and hepatotoxicity. These findings also in away possibly means that male brain cells are more susceptible than female and interestingly liver of females are more sensitive than males in induction of apoptosis by opium.

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References

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