The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Vasorelaxing Activity of Ulmus davidiana Ethanol Extracts in Rats: Activation of Endothelial Nitric Oxide Synthase

  • Cho, Eun-Jung (Infection Signaling Network Research Center, Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University) ;
  • Park, Myoung-Soo (Infection Signaling Network Research Center, Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University) ;
  • Kim, Sahng-Seop (Infection Signaling Network Research Center, Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University) ;
  • Kang, Gun (Infection Signaling Network Research Center, Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University) ;
  • Choi, Sung-A (Infection Signaling Network Research Center, Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University) ;
  • Lee, Yoo-Rhan (Infection Signaling Network Research Center, Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University) ;
  • Chang, Seok-Jong (Department of Physiology, College of Medicine, Seonam University) ;
  • Lee, Kwon-Ho (Department of Physical Therapy, Joongbu University) ;
  • Lee, Sang-Do (Infection Signaling Network Research Center, Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University) ;
  • Park, Jin-Bong (Infection Signaling Network Research Center, Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University) ;
  • Jeon, Byeong-Hwa (Infection Signaling Network Research Center, Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University)
  • Received : 2011.08.30
  • Accepted : 2011.11.13
  • Published : 2011.12.30
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Abstract

Ulmus davidiana var. japonica Rehder (Urticales: Ulmaceae) (UD) is a tree widespread in northeast Asia. It is traditionally used for anticancer and anti-inflammatory therapy. The present study investigated the effect of an ethanol extract of UD on vascular tension and its underlying mechanism in rats. The dried root bark of UD was ground and extracted with 80% ethanol. The prepared UD extract was used in further analysis. The effect of UD on the cell viability, vasoreactivity and hemodynamics were investigated using propidium iodide staining in cultured cells, isometric tension recording and blood pressure analysis, respectively. Low dose of UD ($10{\sim}100{\mu}g/ml)$ did not affect endothelial cell viability, but high dose of UD reduced cell viability. UD induced vasorelaxation in the range of $0.1{\sim}10{\mu}g/ml$ with an $ED_{50}$ value of $2{\mu}g/ml$. UD-induced vasorelaxation was completely abolished by removal of the endothelium or by pre-treatment with L-NAME, an inhibitor of nitric oxide synthase. UD inhibited calcium influx induced by phenylephrine and high $K^+$ and also completely abolished the effect of L-NAME. Intravenous injection of UD extracts (10~100 mg/kg) decreased arterial and ventricular pressure in a dose-dependent manner. Moreover, UD extracts reduced the ventricular contractility (+dP/dt) in anesthetized rats. However, UD-induced hypotensive actions were minimized in L-NAME-treated rats. Taken together, out results showed that UD induced vasorelaxation and has antihypertensive properties, which may be due the activation of nitric oxide synthase in endothelium.

Keywords

References

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