Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Atractylodis Rhizoma Alba on Osteoclast Formation

백출의 파골세포 분화에 미치는 영향

  • Park, Sung-Tae (Department of Internal Medicine, Division of Rheumatology, Wonkwang University) ;
  • Lee, Myeung-Su (Department of Internal Medicine, Division of Rheumatology, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, Collage of Oriental Medicine, Wonkwang University) ;
  • Park, Kie-In (Division of Biological Science, School of Natural Science, Chonbuk National University) ;
  • Oh, Jae-Min (Department of Anatomy, Collage of Medicine, Wonkwang University)
  • 박성태 (원광대학교 의과대학 내과학교실) ;
  • 이명수 (원광대학교 의과대학 내과학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 박기인 (전북대학교 자연과학대학) ;
  • 오재민 (원광대학교 의과대학 해부학교실)
  • Received : 2011.01.04
  • Accepted : 2011.02.14
  • Published : 2011.02.25
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Abstract

Atractylodis Rhizoma Alba is commonly used herbal medicine and it has been known that has immuno-regualtory effects and anti-cancer effects. The inhibition of osteoclastogenesis is essential for the prevention and treatment of osteoporosis. The aim of this study was to evaluate the effects of Atractylodis Rhizoma Alba on osteoclast differentiation in vitro and on resorbing activity of osteoclast. Osteoclast formation was evaluated in bone marrow cells (BMC) in the presence or absence of Atractylodis Rhizoma Alba. The expression of c-fos, tartrate-resistant acid phosphatase (TRAP), OSCAR, DC-STAMP, cathepsin K, MafB and NFATc1 mRNA in osteoclast precursor were assessed by RT-PCR. The levels of TNF receptor-associated factor-6 (TRAF-6), c-fos and NFATc1 protein were assessed by Western blot analysis. Also the correlation with MAPKs and NF-${\kappa}B$ pathways were measured by using Western blot analysis. With bone resorption study, I tried to evaluate the inhibitory effects of Atractylodis Rhizoma Alba on mature osteoclast function. Atractylodis Rhizoma Alba inhibited the RANKL induced osteoclastic differentiation from bone marrow macrophage in a dose dependant manner without cellular toxicity. Gene expression of c-fos and NFATc1 was significantly down regulated with Atractylodis Rhizoma Alba treatment. Atractylodis Rhizoma Alba markedly inhibited the RANKL-induced osteoclastogenesis through suppression of nuclear factor kappa b (NF-${\kappa}B$) pathway, down stream pathway of p38, ERK and JNK pathway. Taken together, I concluded that Atractylodis Rhizoma Alba have beneficial effect on osteoporosis by inhibition of osteoclast differentiation and by inhibition of functioning osteoclast. Thus I expect that Atractylodis Rhizoma Alba could be a treatment option for osteoporosis.

Keywords

References

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