Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Nardostachys Chinensis Induces G0/G1 Phase Cell Cycle Arrest in U937 Cells

감송향(甘松香) 물추출물의 세포주기 정지를 통한 U937세포의 성장억제 효과

  • Kang, Min-Soo (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Ju, Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Jeon, Byung-Jae (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Yang, Hyun-Mo (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kim3, Won-Sin (Division of Natural Science, College of Natural Sciences, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
  • 강민수 (원광대학교 한의과대학 병리학교실) ;
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 전병제 (원광대학교 한의과대학 병리학교실) ;
  • 양현모 (원광대학교 한의과대학 병리학교실) ;
  • 김원신 (원광대학교 자연과학대학 생명과학부) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실)
  • Received : 2011.02.01
  • Accepted : 2011.04.11
  • Published : 2011.04.25
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Abstract

Nardostachys chinensis (N. Chinensis) belonging to the family Valerianaceae have been used in traditional medicine to elicit stomachic and sedative effects. The present study investigated the effects of water extract of N. Chinensis in human lymphoma U937 cells. The proliferation of U937 cells was decreased by N. Chinensis. Anti-proliferative effect of N. Chinensis on U937 cells was associated with G0/G1 phase arrest, which was mediated by regulating the expression of p21 and p27 protein. In addition, the levels of CDK2, CDK4, CDK6, Cyclin D3, and Cyclin A were decreased, but Cyclin D1, Cyclin D2 and Cyclin E were essentially undetectable. N. Chinensis induced the differentiation of U937 as shown by increased expression of differentiation surface antigen CD11b, but not CD14. Taken together, these results demonstrated that N. Chinensis potently inhibits the proliferation of U937 cells via the G0/G1 phase cell cycle arrest in association with p21 and p27, and induces granulocytic differentiation.

Keywords

References

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