Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Analgesic Effects of Sokyungwhalhyul-tang on Constriction Nerve Injury-Induced Neuropathic Pain in Rats

말초 신경병증성 통증 모델에서 소경활혈탕의 진통 효과

  • Received : 2011.02.21
  • Accepted : 2011.04.11
  • Published : 2011.04.25
Download PDF
⟨ Previous Next ⟩

Abstract

Nardostachys chinensis;Anti-proliferation;Cell cycle arrest;Differentiation;U937 cells; This study was conducted to determine the analgesic effect of Sokyungwhalhyul-tang(SKWHT) using the model of peripheral neuropathic pain model. A model of neuropathic pain was made by ligating left 5th lumbar spinal nerve of rats. After 1 days, the extract of SKWHT was orally administered daily. Rats were divided into four groups; (1) Control group(n=6), (2) Experimental group I(SKWHT-OA1, 100 mg/kg, n=6), (3) Experimental group II(SKWHT-OA2, 300 mg/kg, n=6), (4) Experimental group III(SKWHT-OA3, 500 mg/kg, n=6). After that, we examined the withdrawl response of neuropathic rats legs by von Frey filament and Hot plate at pre, $1^{th}$, $4^{th}$, $7^{th}$, $14^{th}$, $21^{th}$ days after the induction of neuropathic pain. And also we examined c-fos, GOT, GPT and histological study of Liver at 21th days. von Frey filament and Hot plate were increase in experimental group I, II, III than Con. especially group III was most significantly analgesic effect than the other groups at $14^{th}$, $21^{th}$ days. In c-fos protein expression on spinal cord, group III was most significantly reduction immunoreactivity at $21^{th}$ days and in blood serum GOT & GPT levels and histologic finding of Liver in all experimental groups were no significant difference with Con at $21^{th}$ days. According to the above results, SKWHT(500 mg/kg) may have a significant analgesic effect on the neuropathic pain.

Keywords

References

  1. Mitchell, S.W. Injuries of nerves and their consequences, Philadelphia: Lippincott, p 252, 1872.
  2. Bonica, J.J. Causalgia and other reflex sympathetic dystrophies, In: Bonica, J.J. editor. The Management of Pain, Philadelphia, Lea and Febiger, pp 220-243, 1990.
  3. Verdugo, R.J., Ochoa, J.L. Use and misuse of conventional electrodiagnosis, quantitative sensory testing, thermography, and nerve blocks in the evaluation of painful neuropathic syndromes. Muscle Nerve, 16: 1056-1082, 1993. https://doi.org/10.1002/mus.880161009
  4. Bowsher, D. Pain syndromes and their treatment. Curr Opin Neurol Neurosurg, 6: 257-263, 1993.
  5. 郭靄春編著. 黃帝內經素問校注譯釋. 서울, 醫聖堂, pp346-369, 1993.
  6. 장경악. 類經(上). 서울, 대성출판사, pp 372-375, 1982.
  7. 대한침구학회 교재편찬위원회. 침구학(중,하). 서울, 집문당, p 176, 230, 2008.
  8. 오창록, 나창수, 유충열, 조명래. 위중, 후계, 위중배후계 전침 및 침자가 백서의 신경병리성 동통 억제에 미치는 영향. 대한침구학회지 22(1):77-90, 2005.
  9. 정정희, 윤대환, 나창수, 유충열, 윤여충, 조명래. 위중, 후계, 위중배후계 침자가 백서의 신경병리성 동통억제 및 c-Fos 단백 발현에 미치는 영향. 대한침구학회지 21(1):240-251, 2004.
  10. 박상민, 이윤호, 강성길. 전침이 신경병리성 냉이질통 발생억제에 미치는 영향. 대한침구학회지 22(6):27-36, 2005.
  11. 김성남, 김성철, 최회강, 소기숙, 임정아, 황우준, 문형철, 최성용, 이상관, 나창수. 環跳穴蜈蚣藥鍼刺戟이 白鼠의 神經病理性 疼痛 抑制에 미치는 影響. 대한침구학회지 21(3):145-167, 2004.
  12. 이동현, 윤대환, 나창수, 윤여충. 환도혈(GB30) 봉독 약침 자극이 백서의 신경병리성 동통 억제에 미치는 영향. 대한침구학회지 22(5):67-77, 2006.
  13. 김선종, 정문재, 박성역, 김정환, 염승룡, 권영달, 송용선. 白屈菜藥鍼이 白鼠의 末梢 神經病症性 疼痛 抑制에 미치는 影響. 한방재활의학과학회지 17(2):1-16, 2007.
  14. 공정현. 萬病回春(下). 서울, 杏林書院, pp 55, 1972.
  15. 이상인. 本草學, 서울, 修書院, p 58, 101, 103, 108, 203, 221, 222, 226, 229, 244, 281, 319, 248, 407, 434, 466, 509, 1981.
  16. 주명신. 醫門寶鑑. 서울, 三協出版社, pp 224-227, 1971.
  17. 박남착. 漢方治療大全. 서울, 南山堂, p 130, 248, 345, 1972.
  18. 박성수, 염태환. 現代漢方講座. 서울, 杏林書院, p 241, 250, 1973.
  19. 염태환. 東醫處方大全. 서울, 東醫溫知堂, p 330, 1970.
  20. 김지혁. 疎經活血湯의 消炎, 鎭痛에 관한 實驗的硏究. 慶熙大學校大學院, 1983.
  21. 이중휘. Type II Collagen으로 誘發된 關節炎에 대한 疎經活血湯加味의 免疫抑制效果. 대전대학교 대학원 박사학위논문, 2004.
  22. Kim, S.H., Chung, J.M. An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat. Pain, 50: 355-363, 1992. https://doi.org/10.1016/0304-3959(92)90041-9
  23. Chaplan, S.R., Malmberg, A.B., Yaksh, T.L. Efficacy of spinal NMDA receptor antagonism in formalin hyperalgesia and nerve injury evoked allodynia in the rat. J Pharmacol Exp Ther, 280: 829-838, 1997.
  24. Merskey H. Prepared by the IASP subcommittee on taxonomy. Classification of chronic pain : descriptions of chronic pain syndromes and definitions of pain terms. Pain Suppl, 3: S1-S226, 1986.
  25. Bonica J.J. In: Merskey H., Bogduk N. eds. Classification of chronic pain. 2nd ED. Seattle, IASP Press. pp 209-314, 1994.
  26. 河北醫學院 編著. 靈樞經校釋(下冊). 北京, 人民衛生出版社, p352, 1982.
  27. 변부형, 임사비나, 이승우. 좌골신경통의 침구치료. 제한동의학술원 논문집, 4(1):558-565, 1999.
  28. 丁光迪主編. 諸病源候論校注(下). 北京, 人民衛生出版社, p 854, 1992.
  29. 南京中醫學院編著. 黃帝內經靈樞譯釋. 上海, 上海科學技術出版社, pp 96-113, 1997.
  30. 황도연. 醫宗損益. 서울, 醫藥社, pp 356-358, 1976.
  31. 이 고. 東垣十書(格致餘論). 서울, 慶熙醫大漢醫學部, pp 5-6, 1973.
  32. 허 준. 東醫寶鑑. 서울, 南山堂, p 372, 1972.
  33. 장개빈. 景岳全書(卷十三). 上海, 衛生出版社, pp 211-212, 1996.
  34. Greenberg, M.E., Greene, L.A., Ziff, E.B. Nerve growth factor and epidermal growth factor induce rapid transient changes in proto-on-cogene transcription in PC12 cells. J Bio Chem, 260: 14101-10110, 1985.
  35. Hunt, S.P., Pini, A., Evan, G. Induction of c-fos like protein in spinal cord neurons following sensory stimulation. Nature, 328: 632-634, 1987. https://doi.org/10.1038/328632a0
  36. Abbadie, C, Besson, J.M. C-fos expression in rat lumbar spinal cord following peripheral stimulation in adjuvant-indeuced arthritic and normal rats. Brain Res, 607: 195-204, 1993. https://doi.org/10.1016/0006-8993(93)91507-O
  37. Laudanna, A., Nogueira, M.I., Mariano, M. Expression of Fos protein in the rat central nerve system in response to noxious stimulation: Effects of chronic inflammation of the superior cervical ganglion. Braz J Med Biol Res, 31: 847-850, 1998. https://doi.org/10.1590/S0100-879X1998000600019