Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Single Dose Toxicity Study of Socheongryong-tang in Sprague-Dawley Rats

Sprague-Dawley 랫드를 이용한 소청룡탕의 단회투여독성시험

  • Lee, Chul-Won (Institute of Marine BioTechnology, School of Korean Medicine, Pusan National University) ;
  • An, Won-Gun (Division of Pharmacology, School of Korean Medicine, Pusan National University)
  • 이철원 (부산대학교 해양생물기술연구소) ;
  • 안원근 (부산대학교 한의학전문대학원 약물의학부)
  • Received : 2011.01.31
  • Accepted : 2011.03.15
  • Published : 2011.04.25
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Abstract

Socheongryong-tang has been used for the treatment of inflammatory allergic diseases such as allergic rhinitis and bronchial asthma in Asian countries. This study was conducted to investigate the safety of Socheongryong-tang in rats. The safety of this tang on acute toxicity was evaluated by single dose toxicity study. Rats were orally administrated in a single dose of 0 and 2000 mg/kg (limited dose) Socheongryong-tang. There were 7 rats in each groups. All animals were sacrificed after 14 days of treatment. After single administration, mortality, clinlcal signs, body weight changes and gross pathological findings were observed for 14 days. Three parameters were tested: organ weight measurement, clinical chemistry, and hematology. In this study with rats, Socheongryong-tang treatment did not show any acute toxicity. No mortality was noted for 14 days of treatment. There were no adverse effects on clinical signs, body weight, organ eight and gross pathological findings at all treatment groups. The clinical chemistry parameters attesting to liver and kidney functions as well as the hematological parameters were within the normal ranges. From single dose toxicity study with rats, it is considered that $LD_{50}$ of Socheongryong-tang is over 2000 mg/kg in oral administration. This finding of the safety on single dose toxicity study of Socheongryong-tang are expected to strengthen the position of Socheongryong-tang as nontoxic medicine.

Keywords

References

  1. Ko, E.J., Rho, S.W., Cho, C.W., Choi, H., Ko, S.G., Lee, Y.W., Hong, M.C., Shin, M.K., Jung, S.G., Bae, H.S. So-Cheong-Ryong-Tang, traditional Korean medicine, suppresses Th2 lineage development. Biol Pharm Bull 27(5):739-743, 2004a. https://doi.org/10.1248/bpb.27.739
  2. Ko, E.J., Rho, S.W., Lee, E.J., Seo, Y.H., Cho, C.W., Lee, Y.W., Min, B.I., Shin, M.K., Hong, M.C., Bae, H.S. Traditional Korean medicine (SCRT) modulate Th1/Th2 specific cytokine production in mice CD4+ T cell. J Ethnopharmacol 92: 121-128, 2004b. https://doi.org/10.1016/j.jep.2004.02.008
  3. 동의학연구소. 새로보는 방약합편. 안양, 도서출판 단샘, pp 236-252, 2006.
  4. 허 준. 신대역 동의보감. 서울, 법인문화사, p 1091, 2009.
  5. Nakao, M., Muramoto, Y., Hisadome, M., Yamano, N., Shoji, M., Fukushima, Y., Saruwatari, J., Nakagawa, K. The effect of Shoseiryuto, a traditional Japanese medicine, on cytochrome P450s, N-acetyltransferase 2 and xanthine oxidase, in extensive or intermediate metabolizers of CYP2D6. Eur J Clin Pharmacol 63: 345-353, 2007. https://doi.org/10.1007/s00228-006-0253-5
  6. Kao, S.T., Wang, S.D., Wang, J.Y., Yu, C.K., Lei, H.Y. The Effect of herbal medicine, xiao-qing-long-tang (XQLT), on allergen-induced bronchial inflammation in mite-sensitized mice. Allergy 55: 1127-1130, 2000. https://doi.org/10.1034/j.1398-9995.2000.00728.x
  7. Kao, S.T., Lin, C.S., Hsieh, C.C., Hsieh, W.T., Lin, J.G. Effects of xiao-qing-long-tang (XQLT) on bronchoconstriction and airway eosinophil infiltration in ovalbumin-sensitized guinea pigs: in vivo and in vitro studies. Allergy 56:1164-1171, 2001. https://doi.org/10.1034/j.1398-9995.2001.00982.x
  8. Bruchajzer, E., Szymanska, J.A., Piotrowski, J.K. Acute and subacute nephrotoxicity of 2-bromophenol in rats. Toxicol Lett 134: 245-252, 2002. https://doi.org/10.1016/S0378-4274(02)00172-8
  9. Fujii, H., Sun, B., Nishioka, H., Hirose, A., Aruoma, O.I. Evaluation of the safety and toxicity of the oligomerized polyphenol oligonol. Food Chem Toxicol 45: 378-387, 2007. https://doi.org/10.1016/j.fct.2006.08.026
  10. Harizal, S.N., Mansor, S.M., Hasnan, J., Tharakan, J.K.J., Abdullah, J. Acute toxicity study of the standardized methanolic extract of Mitragyna speciosa Korth in Rodent. J Ethnopharmacol 131: 404-409, 2010. https://doi.org/10.1016/j.jep.2010.07.013
  11. Mukinda, J.T., Syce, J.A. Acute and cronic toxicity of the aqueous extract of Artemisia afra in Rodent. J Ethnopharmacol 111: 138-144, 2007.
  12. Sheng, Y., Bryngelsson, C., Pero, R.W. Enhanced DNA repair, immune function and reduced toxicity of C-MED-100TM, a novel aqueous extract from Uncaria tomentosa. J Ethnopharmacol 69: 115-126, 2000. https://doi.org/10.1016/S0378-8741(99)00070-7
  13. Topping, D.C., Bernard, L.G., O'Donoghue, J.L., English, J.C. Hydroquinone: Acute and subchronic toxicity studies with emphasis on neurobehavioral and nephrotoxic effects. Food Chem Toxicol 45: 70-78, 2007. https://doi.org/10.1016/j.fct.2006.07.019
  14. 식품의약품안전청. 식품의약품안전청고시 제 2009-116호(2009년 08월 24일) '의약품등의 독성시험기준'. 2009.
  15. 식품의약품안전청. 식품의약품안전청고시 제 2009-102호(2009년 08월 24일) '비임상시험관리기준'. 2009.
  16. OECD. OECD guidelines for testing of chemical no. 423. Acute oral toxicity-Acute class method, line 23. 2001.
  17. 김은순, 장보윤, 이안숙, 강대길, 이호섭, 김병두, 김성연. 랫트에서 한약재 복합물 BDR-29의 단회 경구투여 독성에 관한연구. 생약학회지 38(1):71-75, 2007.
  18. 신인식, 김정훈, 하혜경, 황대선, 허정임, 신현규. Sprague-Dawley 랫드를 이용한 평위산의 안전성 연구. 동의생리병리학회지 24(3):426-429, 2010.
  19. 한혜경, 최성숙, 신진철, 정하숙. 생리활성이 우수한 고품질 유색미의 단회 경구투여 안전성 연구. 한국식품영양과학회지 36(5):527-533, 2007.
  20. 김성민, 석귀덕. 섬백리향 정유의 진통, 항염증, 정신적인 작용. 약학회지 51(6):508-516, 2007.