Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Screening of Herbal Extracts to Reduce PAR-2 and Cytokine Expression Related to Atopic Dermatitis in Keratocytes

피부세포에서 아토피 피부염의 유발과 관련된 PAR-2 및 사이토카인의 발현을 감소시키는 한약재 탐색

  • Park, Sun-Min (Department of Food and Nutrition, College of Natural Science, Basic Science Institutes, Hoseo University) ;
  • Lee, Jung-Bok (Sonimedi) ;
  • Kim, Da-Sol (Department of Food and Nutrition, College of Natural Science, Basic Science Institutes, Hoseo University)
  • 박선민 (호서대학교 자연과학대학 기초과학연구소 식품영양학과) ;
  • 이정복 (소니메디(주)) ;
  • 김다솔 (호서대학교 자연과학대학 기초과학연구소 식품영양학과)
  • Received : 2011.02.08
  • Accepted : 2011.04.11
  • Published : 2011.04.25
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Abstract

The prevalence of atopic dermatitis has markedly increased in recent years but the mechanism has not been clearly revealed. Recent study exhibited that atopic dermatitis was exacerbated by the increase of proteinase-activated receptor (PAR)-2 expression, which activated $I{\kappa}B$ kinase --> nuclear factor kappa B. Therefore, we determined whether the allergens of dust mites induced the expression of PAR-2, intercellular adhesion molecule-1 (ICAM-1, adehision molecule), interleukins (IL)-6 in HaCaT keratocytes and which herbal 1,3-butylene glycol extracts (Mori Cortex Radicis, Sanguisorba officinalis L., Arctium lappa Linne, Torilis japonica DC, Melia azedarach Linne var. japoinca Makino) suppressed their expression. Dust mite allergen increased PAR-2, ICAM-1 and IL-6 expression in HaCaT cells in a dose-dependent manner up to $3{\mu}g/mL$ but their expression reached the plateau over the dosages. The allergen ($3{\mu}g/mL$) also secreted more cytokines such as tumor necrosis factor (TNF)-${\alpha}$ and IL-6 into the media. Among five different herbal extracts ($50{\mu}g/mL$), Mori Cortex Radicis and Sanguisorba officinalis L. suppressed the PAR-2, ICAM-1 and IL-6 expression in HaCaT cells, which was activated by dust mite allergen ($3{\mu}g/mL$) and they also reduced the secretion of TNF-${\alpha}$ and IL-6 into the media. In conclusion, Mori Cortex Radicis and Sanguisorba officinalis L. can effectively reduce the prevalence and progression of atopic dermatitis by dute mite allergen.

Keywords

References

  1. Oh, J.W., Kim, B.S., Lee, J.H., Lee, S.C., Kim, Y.K. The environment and lifestyles of atopic dermatitis patients. Kor J Dermatol. 37: 983-991, 1999.
  2. Sator, P.G., Schmidt, J.B., Honningsmann, H. Comparison of epidermal hydration and skin surface lipids in healthy individuals and in patients with atopic dermatitis. J Am Acad dermatol 48: 352-358, 2003. https://doi.org/10.1067/mjd.2003.105
  3. Matsukura, H., Watanabe, N., Geba, G.P., Sperl, J., Tsudzuki, M., Hiroi, J., Matsumoto, M., Ushio, H., Saito, S., Askenase, P.W., Ra, C. Development of atopic dermatitis-like skin lesion with IgE hyperproduction in NC/Nga mice. Int Immunol 3: 461-466, 1996.
  4. Park, S.H., Jin, M.R., Koo, Y.S., Kim, D.H. Effect of BHOSB on various immunological factors related to pathogenesis of atopic dermatitis in DNCB treated NC/Nga mice. J Kor Orien Physiol & Pathol. 21: 849-855, 2007.
  5. Kato, T., Takai, T., Fujimura, T., Matsuoka, H., Ogawa, T., Murayama, K., Ishii, A., Ikeda, S., Okumura, K., Ogawa, H. Mite serine protease activates protease-activated receptor-2 and induces cytokine release in human keratinocytes. Allergy. 64: 1366-1374, 2009. https://doi.org/10.1111/j.1398-9995.2009.02023.x
  6. Tsujii, K., Andoh, T., Ui, H., Lee, J.B., Kuraishi, Y. Involvement of tryptase and proteinase-activated receptor-2 in spontaneous itch-associated response in mice with atopy-like dermatitis. J Pharmacol Sci. 109: 388-395, 2009. https://doi.org/10.1254/jphs.08332FP
  7. Lee, Y.H., Kim, M.J., Kong, I.D., Ryu, J.S., Jang, M.Y., Lee, C.G., Choi, E.H. Clinical improvement of atopic dermatitis by a topical cream containing a protease-Activated Receptor-2 (PAR-2) inhibitor "Pal-KTTKS peptide". Kor J Dermatol. 48: 966-974, 2010.
  8. Guin, J.D. Complications of topical hydrocortisone. J Am Acid Dermatol. 4: 417-422, 1981. https://doi.org/10.1016/S0190-9622(81)70040-9
  9. Heo, J.C., Lee, K.Y., Lee, B.G., Choi, S.Y., Lee, S.H., Lee, S.H. Anti-allergic activities of ultra-fine powder from persimmon. Kor J Food Presev. 17: 145-150, 2010.
  10. Shin, Y.K., Heo, J.C., Lee, J.H., Lee, S.H. Analysis of the anti-allergic activities of active components produced by solid fermentation of Phellinus baumii and Ephedra sinica. Kor J Food Presev. 17: 297-300, 2010.
  11. Ju, J.H., Cho, H.H., Lee, Y.S. Progress on phytochemical and atopic dermatitis-related study of the root of Lithospermum erythrorhizon. Kor J Pharmacog. 41: 73-88, 2010.
  12. Kim, J.Y., Jeong, M.S., Choi, S.E., Kim, J.Y., Park, K.Y., Park, K.H., Lee, D.I., Joo, S.S., Lee, C.S., Bang, H.W., Lee, M.K. The effects of Acer ginnala leaves extraction on the atopic dermatitis-like skin lesions in NC/Nga mice. Kor J Dermatol. 48: 913-918, 2010.
  13. Joo, Y.H., Won, C.H., Kim, J.Y., Cho, K.H., Min, K.U., Kim, K.H. Developing an atopic dermatitis model and the effects of Actinidia extract on dermatitis in NC/Nga mice. Kor J Dermatol. 47: 1105-1112, 2009.
  14. Ahn, J.Y., Im, L.R., Kim, J.H., Park, J.H., Kim, D.K., Lee, Y.M. Effects of Rumecis Radix water extract on development of atopic dermatitis in BALB/c mice. Kor J Pharmacog. 40: 218-223, 2009.
  15. D'Andrea, M.R., Derian, C.K., Leturcq, D., Baker, S.M., Grunmark, A., Ling, P., Darrow, A.L., Santulli, R.J., Brass, L.F., Andrade-Gordon, P. Characterization of protease-activated receptor-2 immunoreactivity in normal human tissues. J Histochem Cytochem. 46: 157-164, 1998. https://doi.org/10.1177/002215549804600204
  16. Sharlow, E., Paine, C., Babiarz, L., Eisinger, M., Shapiro, S., Seiberg, M. The protease-activated receptor-2 upregulates keratinocyte phagocytosis. J Cell Sci. 113: 3093-3101, 2000.
  17. Rattenholl, A., Steinhoff, M. Role of proteinase-activated receptors in cutaneous biology and disease. Drug Dev Res. 59: 408-416, 2003. https://doi.org/10.1002/ddr.10311
  18. Nystedt, S., Ramakrishnan, V., Sundelin, J. The proteinase-activated receptor 2 is induced by inflammatory mediators in human endothelial cells: comparison with the thrombin receptor. J Biol Chem. 271: 14910-14915, 1996. https://doi.org/10.1074/jbc.271.25.14910
  19. Holzhausen, M., Spolidorio, L., Vergnolle, N. Roles of protease-activated receptor-2 in inflammation, and its possible implications as a putative mediator of periodontitis. Mem Inst Oswaldo Cruz. 100: 177-180, 2005.
  20. Darsow, U., Laifaoui, J., Kerschenlohr, K., Wollenberg, A., Przybilla, B., Wuthrich, B., Brönnimann, M., Braathen, L.R., Didierlaurent, A., André, C., Ring, J. The prevalence of positive reactions in the atopy patch test with aeroallergens and food allergens in subjects with atopic eczema: a European multicenter study. Allergy 59: 1318-1325, 2004. https://doi.org/10.1111/j.1398-9995.2004.00556.x
  21. Lee, S.E., Jeong, S.K., Lee, S.H. Protease and protease-activated receptor-2 signaling in the pathogenesis of atopic dermatitis. Yonsei Med J. 51: 808-822, 2010. https://doi.org/10.3349/ymj.2010.51.6.808
  22. Jeong, S.K., Kim, H.J., Youm, J.K., Ahn, S.K., Choi, E.H., Sohn, M.H., Kim, K.E., Hong, J.H., Shin, D.M., Lee, S.H. Mite and cockroach allergens activate protease-activated receptor 2 and delay epidermal permeability barrier recovery. J Invest Dermatol. 128: 1930-1939, 2008. https://doi.org/10.1038/jid.2008.13
  23. Park, S.Y., Kim, J.S., Lee, B.H., Lim, S.C., Lee, S.N., Leem, K.H., Lee, K.M. Whitening effects of Mori ramulus, Mori cortex radicis and Mori folium herbal-acupuncture solution after fermentation and heating. J Korean Acumoxa Soc. 26: 91-98, 2009.
  24. Yu, J.Y., Park, S.H., Jo, S.J., Heo, C.H., Yun, S.U., Park, G.C. A Clinical study on the effect of a cream containing Ramulus mori extract and tea tree oil on acne vulgaris and aerobic skin flora. Kor J Dermatol. 41: 1136-1141, 2003.
  25. Yoon, C.Y., Shin, D.H., Hong, C.M., Lee, W.K., Jang, D.D., Cho, J.C., Ahn, J.K., Ahn, D.K., Lee, M.S. The Suppressive effects of cortex Mori on NO , TNF-${\alpha}$ and IL-1 production by macrophage. Kor J Vet Public Health. 22: 281-292, 1998.
  26. An, B.J., Lee, S.A., Son, J.H., Gwag, J.H., Park, J.M., Lee, J.Y. Cytotoxic and antibacterial activities of Sanguisorbae officinalis L. J Appl Biol Chem. 47: 141-145, 2004.
  27. An, B.J., Lee, J.T., Lee, S.A., Kwak, J.H., Park, J.M., Lee, J.Y., Son, J.H. Antioxidant effects and application as natural ingredients of Korean Sanguisorbae officinalis L. J Appl Biol Chem. 47: 244-250, 2004.
  28. Lee, E.B., Cho, S.I., Kang, S.S., Kim, K.R., Kim, T.H. Isolation of Torilin from Torilis japonica fruit and its analgesic and anti-inflammatory activities. Kor J Pharmacog. 30: 137-144, 1999.
  29. Koo, B.S., Hwang, E.I., So, S.H., Lee, S,K., Han, G.H., Kim, N.M. Effect of Torilis fructus on procollagen biosynthesis and activity of matrix metalloproteinase-I(MMP-1) in human dermal fibroblast. Kor J Pharmacog. 38: 349-353, 2007.
  30. Yoo, H.C., Kim, S.H., Kim, D.H. An experimental study of Arctii Fructus on the antiallergic effect. Kor J Herbology. 16: 111-128, 2001.
  31. Shin, D.W., Kim, M.S., Han, J.S. Antimicrobial effect of ethanol extracts from some medicinal herbs and their fractionates against food -born bacteria. Kor J Food Sci. 29: 808-816, 1997.
  32. Yi, H.S., Heo, S.K., Yun, H.J., Kim, B.W., Park, S.D. Anti-oxidative and anti-inflammatory effect of Melia toosendan in mouse macrophage cells. Kor J Hebalogy. 23: 121-134, 2008.