Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Red Ginseng-Ejung-tang on Nitric Oxide and Hydrogen Peroxide Production in LPS-induced Mouse Macrophages RAW 264.7

홍삼이중탕(紅蔘理中湯)이 LPS로 유발된 마우스 대식세포 RAW 264.7의 nitric oxide 및 hydrogen peroxide 생성에 미치는 영향

  • Lee, Ji-Young (Department of Pathology, College of Oriental Medicine, Kyungwon University) ;
  • Park, Wan-Su (Department of Pathology, College of Oriental Medicine, Kyungwon University)
  • 이지영 (경원대학교 한의과대학 병리학교실) ;
  • 박완수 (경원대학교 한의과대학 병리학교실)
  • Received : 2011.03.02
  • Accepted : 2011.04.14
  • Published : 2011.04.25
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Abstract

The purpose of this study is to investigate effects of Red Ginseng-Ejung-tang (RE) on nitric oxide (NO) and hydrogen peroxide production in RAW 264.7 mouse macrophages induced by lipopolysaccharide (LPS). Cell viability was measured by modified MTT assay. NO production was measured by Griess reagent assay. Hydrogen peroxide production was measured by dihydrorhodamine 123 (DHR) assay. RE did not show cell toxicity against RAW 264.7 for 24 hr incubation at the concentrations of 10, 25, 50, 100, and $200{\mu}g/mL$ in RAW 264.7. RE significantly inhibited NO production for 24 hr incubation at the concentrations of 10, 25, 50, and $100{\mu}g/mL$ in RAW 264.7 (P < 0.05). RE significantly inhibited the LPS-induced production of NO for 24 hr incubation at the concentrations of 10, 25, 50, and $100{\mu}g/mL$ in RAW 264.7 (P < 0.05). RE significantly inhibited the LPS-induced production of hydrogen peroxide for 16, 24, 40, 48, 64, and 72 hr incubation at the concentrations of 50, 100, and $200{\mu}g/mL$ in RAW 264.7 (P < 0.05). These results suggest that RE has anti-inflammatory property related with its inhibition of NO and hydrogen peroxide production in LPS-induced macrophages.

Keywords

References

  1. 顧武軍, 張民慶主 編, 김동희 외 15인 공편역. 현대상한론. 서울, 한의문화사, pp 647-649, 2000.
  2. 廣州中醫學院 主編, 이상인, 김동찬, 이영종, 노승현, 주영승 공편역. 方劑學. 서울, 영림사, pp 144-146, 1990.
  3. Jiang, J.P., Fu, Y., Hong, Y.G. Bovine adrenal medulla 22 attenuates hyperalgesia in the early phase of complete Freund's adjuvant-induced inflammation in rats. Sheng Li Xue Bao. 63(1):9-19, 2010.
  4. Tufekci, K.U., Genc, S., Genc, K. The endotoxin-induced neuroinflammation model of Parkinson's disease. Parkinsons Dis. 2011: 487450, 2011.
  5. Gupta, Y.K., Chauhan, A. Potential of immunosuppressive agents in cerebral ischaemia. Indian J Med Res. 133(1):15-26, 2011.
  6. Hines, I.N., Grisham, M.B. Divergent roles of superoxide and nitric oxide in liver ischemia and reperfusion injury. J Clin Biochem Nutr. 48(1):50-56, 2011.
  7. Sun, B., Lu, C., Zhou, G.P., Xing, C.Y. Suppression of Par-4 Protects Human Renal Proximal Tubule Cells from Apoptosis Induced by Oxidative Stress. Nephron Exp Nephrol. 117(3):e53-e61, 2010.
  8. Graber, D.J., Park, P.J., Hickey, W.F., Harris, B.T. Synthetic triterpenoid CDDO derivatives modulate cytoprotective or immunological properties in astrocytes, neurons, and microglia. J Neuroimmune Pharmacol. 6(1):107-120, 2011. https://doi.org/10.1007/s11481-010-9240-9
  9. Melillo, A.A., Bakshi, C.S., Melendez, J.A. Francisella tularensis antioxidants harness reactive oxygen species to restrict macrophage signaling and cytokine production. J Biol Chem. 285(36):27553-27560, 2010. https://doi.org/10.1074/jbc.M110.144394
  10. Fila, L., Musil, J. Examination of exhaled breath condensate in cystic fibrosis. Cas Lek Cesk. 149(4):173-177, 2010.
  11. 박완수. 白蔘과 紅蔘이 포함된 理中湯의 마우스 대식세포 내 hydrogen peroxide 생성에 미치는 영향. 동의생리병리학회지 25(1):78-83, 2011.
  12. 박완수. 마우스 대식세포(RAW 264.7)에 대한 艾葉물추출물의 생리활성 연구. 동의생리병리학회지 22(4):815-820, 2008.
  13. Jirapongsananuruk, O., Malech, H.L., Kuhns, D.B., Niemela, J.E., Brown, M.R., Anderson-Cohen, M., Fleisher, T.A. Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay. J Allergy Clin Immunol. 111(2):374-379, 2003. https://doi.org/10.1067/mai.2003.58
  14. Richardson, M.P., Ayliffe, M.J., Helbert, M., Davies, E.G. A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test. J Immunol Methods. 219(1-2):187-193, 1998. https://doi.org/10.1016/S0022-1759(98)00136-7
  15. McLean, S., Bowman, L.A., Poole, R.K. KatG from Salmonella typhimurium is a peroxynitritase. FEBS Lett. 584(8):1628-1632, 2010. https://doi.org/10.1016/j.febslet.2010.03.029
  16. Childs, E.W., Tharakan, B., Hunter, F.A., Smythe, W.R. 17beta-estradiol mediated protection against vascular leak after hemorrhagic shock: role of estrogen receptors and apoptotic signaling. Shock. 34(3):229-235, 2010. https://doi.org/10.1097/SHK.0b013e3181d75b50
  17. Farrell, H., Hayes, J., Laffey, J., Rowan, N. Studies on the relationship between pulsed UV light irradiation and the simultaneous occurrence of molecular and cellular damage in clinically-relevant Candida albicans. J Microbiol Methods. 84(2):317-326, 2011. https://doi.org/10.1016/j.mimet.2010.12.021
  18. Freitas M, Porto G, Lima JL, Fernandes E. Optimization of experimental settings for the analysis of human neutrophils oxidative burst in vitro. Talanta. 78(4-5):1476-1483, 2009. https://doi.org/10.1016/j.talanta.2009.02.050