Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Water Extract of Saussureae Radix in RANKL-induced Osteoclast Differentiation

파골세포 분화에 목향 물 추출물의 효과

  • Lee, Myeung-Su (Department of Internal Medicine, Division of Rheumatology and Institute of Wonkwang Medical Science) ;
  • Kim, Jeong-Joong (Department of Anatomy, University of Wonkwang College of Medicine) ;
  • Oh, Jae-Min (Department of Anatomy, University of Wonkwang College of Medicine) ;
  • Choi, Min-Kyu (Department of Anatomy, University of Wonkwang College of Medicine) ;
  • Song, Mi-Jin (Department of Anatomy, University of Wonkwang College of Medicine) ;
  • Ahn, Yong-Hwan (Department of Anatomy, University of Wonkwang College of Medicine) ;
  • Lee, Jeong-Hugh (Department of Anatomy, University of Wonkwang College of Medicine) ;
  • Jeon, Byung-Hoon (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Park, Kie-In (Division of Biological Science, School of Natural Science, Chonbuk National University) ;
  • Jang, Sung-Jo (Department of Neurosurgery, University of Wonkwang College of Medicine)
  • 이명수 (원광대학교 의과대학 류마티스내과학교실 및 원광의과학 연구소) ;
  • 김정중 (원광대학교 의과대학 해부학교실 및 환경과학) ;
  • 오재민 (원광대학교 의과대학 해부학교실 및 환경과학) ;
  • 최민규 (원광대학교 의과대학 해부학교실 및 환경과학) ;
  • 송미진 (원광대학교 의과대학 해부학교실 및 환경과학) ;
  • 안용환 (원광대학교 의과대학 해부학교실 및 환경과학) ;
  • 이정휴 (원광대학교 의과대학 해부학교실 및 환경과학) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 박기인 (전북대학교 자연과학대학 생물학과) ;
  • 장성조 (원광대학교 의과대학 신경외과학교실)
  • Received : 2011.05.06
  • Accepted : 2011.06.09
  • Published : 2011.06.25
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Abstract

Osteoporosis is social problem around the world, because fracture of old age may lead to critical medical condition. Osteoclast is a main target for prevention and treatment of osteoporosis due to their responsibility for bone resorption. Saussureae Radix has been known that has gastro-protective, bronchodilatory effect and has a anti-biotic effect. Saussureae Radix has been widely used in Oriental medicine. However, the effect of extract of Saussureae Radix in osteoclast differentiation remains unknown. Thus, we examined the effect of Saussureae Radix in receptor activator of nuclear factor-${\kappa}$B ligand (RANKL)-induced osteoclast differentiation. From the results of our study, Here we found that Saussureae Radix significantly inhibited osteoclast differentiation induced by RANKL. Saussureae Radix suppressed the activation of NF${\kappa}$B in bone marrow macrophages (BMMs) treated with RANKL. Also, Saussureae Radix significantly inhibited the mRNA expression of c-Fos, tartrate-resistant acid phosphatase (TRAP), osteoclast-associated receptor (OSCAR), nuclear factor of activated T cells (NFAT)c1 and cathepsin K in BMMs treated with RANKL. Particularly, Saussureae Radix greatly inhibited the protein expression of c-fos and NFATc1. especially in the case of NFATc1 expression, a master transcription factor of the differentiation of osteoclasts is very important step for osteoclastogenesis. These results demonstrate that Saussureae Radix may be useful treatment option of bone-related disease such as osteoporosis and rheumatoid arthritis.

Keywords

References

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