The Korean Journal of Physiology and Pharmacology

  • 제15권3호
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  • Pages.129-135
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  • 2011
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  • 1226-4512(pISSN)
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  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
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5-Hydroxytryptamine Generates Tonic Inward Currents on Pacemaker Activity of Interstitial Cells of Cajal from Mouse Small Intestine

  • Shahi, Pawan Kumar (Department of Physiology, College of Medicine, Chosun University) ;
  • Choi, Seok (Department of Physiology, College of Medicine, Chosun University) ;
  • Zuo, Dong Chuan (Department of Physiology, College of Medicine, Chosun University) ;
  • Yeum, Cheol-Ho (Department of Physiology, College of Medicine, Chosun University) ;
  • Yoon, Pyung-Jin (Department of Physiology, College of Medicine, Chosun University) ;
  • Lee, Jun (Department of Internal Medicine, College of Medicine, Chosun University) ;
  • Kim, Young-Dae (Department of Internal Medicine, College of Medicine, Chosun University) ;
  • Park, Chan-Guk (Department of Internal Medicine, College of Medicine, Chosun University) ;
  • Kim, Man-Yoo (Department of Internal Medicine, College of Medicine, Chosun University) ;
  • Shin, Hye-Rang (Department of Psychiatry, College of Medicine, Chosun University) ;
  • Oh, Hyun-Jung (Department of Psychiatry, College of Medicine, Chosun University) ;
  • Jun, Jae-Yeoul (Department of Physiology, College of Medicine, Chosun University)
  • 투고 : 2011.04.08
  • 심사 : 2011.06.14
  • 발행 : 2011.06.30
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초록

In this study we determined whether or not 5-hydroxytryptamine (5-HT) has an effect on the pacemaker activities of interstitial cells of Cajal (ICC) from the mouse small intestine. The actions of 5-HT on pacemaker activities were investigated using a whole-cell patch-clamp technique, intracellular $Ca^{2+}$ ($[Ca^{2+}]_i$) analysis, and RT-PCR in ICC. Exogenously-treated 5-HT showed tonic inward currents on pacemaker currents in ICC under the voltage-clamp mode in a dose-dependent manner. Based on RT-PCR results, we found the existence of 5-$HT_{2B,\;3,\;4,\;and\;7}$ receptors in ICC. However, SDZ 205557 (a 5-$HT_4$ receptor antagonist), SB 269970 (a 5-$HT_7$ receptor antagonist), 3-tropanylindole - 3 - carboxylate methiodide (3-TCM; a 5-$HT_3$ antagonist) blocked the 5-HT-induced action on pacemaker activity, but not SB 204741 (a 5-$HT_{2B}$ receptor antagonist). Based on $[Ca^{2+}]_i$ analysis, we found that 5-HT increased the intensity of $[Ca^{2+}]_i$. The treatment of PD 98059 or JNK II inhibitor blocked the 5-HT-induced action on pacemaker activity of ICC, but not SB 203580. In summary, these results suggest that 5-HT can modulate pacemaker activity through 5-$HT_{3,\;4,\;and\;7}$ receptors via $[Ca^{2+}]_i$ mobilization and regulation of mitogen-activated protein kinases.

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